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Antibacterial Activity of Commiphora Africana Extracts Against Some Bacteria Associated with Deterioration of Infected Wound

Ja'afar S. Adam, Shamsu Adnan M and Muhammad Ali*

Abstract

The study was aimed to investigate the phytochemical constituents and antibacterial activity of Commiphora africana leaf extracts against some bacteria associated with infected wound. Aqueous and ethanol extracts from Commiphora africana stem bark were prepared, screened for phytochemicals analysis and tested for antibacterial activity against Staphylococcus aureus, Klebsiella, Pseudomonas aeruginosa and Escherichia coli. Phytochemical screening of the extracts showed the presence of Alkaloid, saponin, tannin, phenol, steroid, flavonoid, terpenoid and glycoside. The result of antibacterial activity showed that ethanol extract demonstrated highest antibacterial activity with average zone of inhibition of 14.5 mm among the isolates than aqueous extracts (13.2 mm). Based on the susceptibility of the organisms to the extracts, E. coli was found to be the highest susceptible organisms with average zone of inhibition of 14.9 mm, followed by Klebsiella (14.2 mm), Pseudomonas (13.9 mm), and while least average zone of inhibition is shown by S. aureus (12.4 mm). The MIC and MBC of the extracts ranges from 3.125 to 50 mg/ml There is no significant different on the susceptibility of the organisms against the extracts at p<0.05 The results of the present study have provided the justification for therapeutic potential of Commiphora africana stem bark extracts against wound isolates.

Keywords

Antibacterial activity Commiphora africana Bacteria Phytochemicals wound

References

Adebayo, A.H., Aliyu, R., Gatsing, D.and Garba, I.H. (2006). The Effects of Ethanolic Leaf Extract of Commiphora africana (Burseraceae) on Lipid Profile in Rats. International Journal of Pharmacology 2: 618-622. Ahmed, I. and Beg, A. Z. (2001). Antimicrobial and phytochemical studies on 45 Indian Medicinal plants against multi-drug resistance human pathogens. Journal of Ethnopharmacol. 2001;74, 113-123. Akor, J.S. and Anjorin, T.S. (2009). Phytochemical and antimicrobial studies of Commiphora africana root extracts. International Journal Agriculture and Biology 2009;11: 795-797. Ali, M., Yahaya, A., Zage, A.U. et al. (2017). In-vitro Antibacterial Activity and Phytochemical Screening of Psidium guajava on Some Enteric Bacterial Isolates of Public Health Importance. (2017) J Advances in Med Pharmaceutical Sci 2017;12(3): 1-7. Amin, M. M., Sawhney, S. S. and Jassal, M. S. (2013). Qualitative and quantitative analysis of phytochemicals of Taraxacum officinale. Wudpecker Journal of Pharmaceutical and Pharmacology. 2013;2(1), 001-005. Bakari, G., Max, R., Mdegela, H., et al. (2012). Effect of crude extracts from Commiphora swynnertonii (Burtt) against selected microbes of animal health importance. J Med Plants Res 2012;6:1795-9. Banso, A and Mann, A. (2006). Antimicrobial alkaloid fractions from Commiphora africana. Journal of pharmacy and Biores. 2006;3(2):98-102. Cheesbrough M. (2012). District laboratory practice in tropical countries, second edition, part two, Cambridge University Press, London, Doughari, J. H., El Mahmud, A. M and Manzara, S. (2008). Studies on the antibacterial activities of root extract of Carica papaya L. African Journal of Microbiology Research. 2007;Pp. 037-041. Edeoga, H. O., Omobuna, G. and Uche, L. C. (2006). Chemical composition of Hyotissu aveoleus and Ocimum gratissium hybrids from Nigeria. African Journal of Biotechnology, 2006;5(910), 892-895. Ezekiel, I., Mabrouk, M.A and Ayo, J.O. (2010). Study of the effect of hydroethanolic extract of Commiphora africana (stem-bark) on inflammation and pain in rodents. Asian J Med Sci 2010;2(3): 81-4. Gao, X., Ohlander, M., Jeppsson, N., Björk, L. and Tra-jkovski, V. (2000). Changes in antioxydant effects and their relationship to phytonutrients in fruits of sea buck- thorn (Hippophaerhamnoides L) during maturation. Journal of Agricultural and Food Chemistry, 2000;48, 1485-1490. doi:10.1021/jf991072g. Idris, M. M. and Usman, S.J. (2018). Antimicrobial Activity of Leaf Extracts of Commiphora africana Bayero Journal of Pure and Applied Sciences, 2018;11(1): 191 194. http://dx.doi.org/10.4314/bajopas.vlli1.31S. Ibrahim, T. A., Dada, I. B. O. and Adejare, R. A. (2010). Comparative phytochemical properties of crude ethanolic extracts and physicochemical characteristics of essential oils of Myristical fragrans (nutmeg) seeds and Zingiber officinate (ginger) roots. Electronic Journal of Environment, Agriculture, Food and Chemical 2010;9(6): 1110-1116. Ibrahim, T., Gidado, Muluh, K., Alexander, A. (2016). Phytochemical Screening, Antioxidant and Antibacterial Activities of Commiphora kerstingii Int. Biol. Biomed. J. Summer 2016; Vol 2, No 3;Pp 127-133. Lifongo, LL, Simoben, CV, Ntie-Kang, F, Babiaka, SB and Judson PN. (2014) A bioactivity versus ethnobotanical survey of medicinal plants from Nigeria, West Africa. Nat Prod Bioprospect; 4:1-19. Nasir, B, Fatima, H, Ahmed M, and Haq, IU. (2015) Recent trends and methods in antimicrobial drug discovery from plant sources. Austin J Microbiol;1:1002. Natarajan, V., Veugopal, P.V. and Menon, T. (2003). Effect of Azadirachta indica (neem) on the growth pattern of dermatophytes. Indian J. Med. Microbiol., 2003;21: 98-101. National Population Commission (NPC) (2014). Nigeria Demographic and Health Survey Abuja, Nigeria. Nomicos, E.Y. (2007). Myrrh," Holistic Nursing Practice, vol. 21, no. 6, pp. 308-323. Ohadoma, S.C., Nnatuanya, I., Amazu, L.U., Okolo, C.E. (2014). Antimicrobial activity of the leaf extract and fractions of Lupinus arboreus, Journal of Medicinal Plants Research. 2014;8(8): 386-391. Okwu, D. E. (2001). Evaluation of the chemical composition of indigenous spices and flavoring agents. Global Journal of Pure and Applied Sciences, 2001;7(3), 455-459. Paraskeva, M. P., van Vuuren, S. F., van Zyl, R. L., et al. (2008). The in vitro biological activity of selected South African Commiphora species. J Ethnopharmacol. 2008;119:673-9. Prohp, T. P. and Onoagbe, I. O. (2013). Determination of phytochemical composition of the stem bark of triplochiton scleroxylon k. schum. (sterculiaceae). International Journal of Applied Biology and Pharmaceutical Technology, 2012;3(2), 68-76. Rabi, T. and Bishayee, A. (2009). Terpenoids and breast cancer chemoprevention. Breast Cancer Research Treatment. 2009;115, 223-239. Sofowora A. (1993). Medicinal Plants and Traditional Medicine in Africa; John Wiley and Sons, Ltd, Ife, Nigeria, 1993;p. 55-201. Toma I, Gidado, Khan M and Abel A. (2016) Phytochemical Screening, Antioxidant and Antibacterial Activities of Commiphora kerstingii. Int. Biol. Biomed. J. Summer; 2-3. Trease, G.E. and Evans, W.C. (2002). Phytochemicals. In: Pharmacognosy. 15th ed. Saunders Publishers, London 2002;42-44, 221-229, 246-249, 304-306,331-332, 391-393. Wikipedia (2021). Kano State. Retrieved from https://en.m.wikipedia.org/wiki/Kano State.