Abstract
Research experiment was conducted at Rivers State University Teaching and Research Farm, Faculty of Agriculture on potential of Bio-augmented fungi-bacterial effective- microorganisms for bioremediation of crude oil contaminated soils. Two factor experiments consisting of five levels of bio-augmented fungi-bacterial effective-microorganisms (BFBE) and a level of Bonny light crude oil were replicated five times and fitted into a Completely Randomized Design (CRD). The treatments were incubated and monitored monthly for three months and samples collected for Laboratory analysis of soil chemical and physical properties. The results from the Laboratory were statistically analyzed using analysis of variance (ANOVA) in a Minitab software and the means separated using a Standard Error at p<0.05. Results showed that BFBE applications increases soil microbial populations such as bacterial count, hydrocarbon utilizing bacterial, fungi population, hydrocarbon utilizing fungi. Bacterial isolated in BFBE treated soil are Bacillus sp, Corrynebacterium, Enterobacter, Staphylococcus, Rhodococcus rhodochrous, Cyanobacteria, Escherichia, Pseudomonas sp., Spirillum sp., Serratia sp., Streptomyces sp. Whereas, fungi such as Aspergillus niger , mucus, Aspergillus fumigates, Fusarum, and chlamydosporum which are found to utilize hydrocarbon bioaugmented fungi-bacterial effective-microorganisms should be used at 100ml to 200ml per liter of water to enhance and improve soil microbial properties.
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