Chemical Composition of Broccoli Leaf Meal and Its Effects on Performance, Carcass, Haematology, and Serum Metabolites in Broiler Chickens
Samuel David Sudik, Umar Sanda Abubakar, Ahmadu Mohammed, Ahmad Muazu, Kosoro, Aminu Mandala, Adamu Lawan and Iliya Bagudu Amaza
Abstract
This study aimed to analyze the chemical composition of broccoli leaf meal and its effects on performance, carcass, haematology, and serum metabolites in broiler chickens. After drying under a shed, broccoli leaves were ground to produce broccoli leaf meal (BLM), which was then analyzed for proximate composition and mineral content. Two basal diets for broilers were formulated and supplemented with varying levels of BLM: 0% (control), 2.5% (A1), 5% (A2), 2.5% (B1), and 5% (B2). In diets A1 and A2, BLM was added without altering other ingredients, while in B1 and B2, adjustments were made to maintain crude protein and energy levels similar to the control. A total of 150 ROSS 308 broiler chicks were used, divided into five groups (30 chicks per group with three replicates each). After arrival, they received anti-stress treatment for three days and were brooded for two weeks, with feed and water available ad libitum for 42 days. The study focused on parameters such as growth rate, feed intake, carcass and organ weights, and blood profile assessment. Results indicated that carbohydrates, potassium, calcium, and iron levels were notably high in the leaves. Body weight was not significantly affected (p>0.05); however, the diet with A2 demonstrated the highest feed intake and mortality rate, along with significantly lower red blood cells, packed cell volume, and hemoglobin (p<0.05), but groups B1 and B2 displayed improved feed conversion ratios. It was concluded that BLM can be included in broiler diets up to 5% if other ingredients are modified to meet protein and energy needs.
Keywords
References
Howitz Editor 17th Edition, Washington, D.C.
Asaolu SS, Adefemi OS, Oyakilome IG, Ajibulu KE, Asaolu MF. Proximate and Mineral
Composition of Nigerian Leafy Vegetables. Journal of Food Research. 2012; 1(3):214-218.
Benjami, M. M. (1998). Outline of Veterinary Clinical Pathology. 2nd ed. Lower State University
press, Lower, USA. 35-105
Edelman, M. and Colt, M. (2016). Nutrient value of leaf vs. seed. Front Chem., 4:1-32.
FAO. The State of Food Security and Nutrition in the World 2021. Transforming food systems for
food security, improved nutrition and affordable healthy diets for all. Rome, FAO.
https://doi.org/10.4060/cb4474en (2021).
Gopalan C, Sastri R, Balasubramanian SC. Nutritive value of Indian foods. National Institute of
Nutrition, ICMR, Hyderabad, 2004, 52.
Hien, T. Q., Hoan, T. T., Khoa.,M. A., Kien, T. T. and Trung., T. Q. (2017). The effect of some
leaf meal kinds as a supplement in the basal diet on luong phuong broiler performance.
Bulgarian Journal of Agricultural Science, 23 (No 4) 2017, 617–624.
Kumar, D., Kumar, S. and Shekhar, C. (2020). Nutritional components in green leafy vegetables:
A review. Journal of Pharmacognosy and Phytochemistry 2020; 9(5): 2498-2502
Lima M, Fontanetti A, Soares M, Gazaf
Lima M, Fontanetti A, Soares M, Gazaffi R, de França Guimarães N, de Souza Gallo A. Vegetative
Development and Nutrient Absorption March of Sorrel (Rumex acetosa L.).Journal of
Agricultural Science. 2019;11(4): 262–70.
Morshed, M. M., Rana, M. S., Emran, T. B., Sohel, M. D. & Kawsar, M. H. Nutritional analysis
and mineral content determination of Emilia sonchifolia DC. Bangladesh Pharm. J. 24(1),
54–60 (2021).
Patricia, Q., Zoue, L., Megnanou, R., Doue, R. and Niamke, S. (2014). Proximate composition
and nutritive value of leafy vegetables consumed in northern côte D’IVOIRE. European
Scientific Journal, 10(6): 212 – 227.
Rahman, S., Aziz, R. and and Farhana, S. (2021). Proximate Compositions and Elemental Analysis
of Leaf, Root, Stem, and Fruit of Bridelia Stipularies. Research Journal of Pharmaceutical,
Biological and Chemical Sciences, 12(3): 30-38.
Samuel David Sudik, Mohammed Aishatu Zangina, Aminu Maidala (2024a). Egg Production and
Quality of Japanese Quails (Coturnix japonica) Fed Diets with various Levels of Pawpaw
Pomace Meal (Carica papaya L.). Journal of Agriculture, Food, Environment and Animal
Sciences. 5(2): 221-231, at: http://www.jafeas.com
Samuel David Sudik, Olayinka John Makinde, Tijjani Lawan, Aminu Maidala (2024b). Effect of
Awara Waste (Soybean By-Product) on Haematology and Serum Biochemical Parameters
of Broiler Chickens. Journal of Agriculture, Food, Environment and Animal Sciences, 5(1):
37-49, at: http://www.jafeas.com/
Sudik, S. D., Ijarotimi, O. S., Agbede, J. O. and Igbasan, F. A. (2019). Nutritional Composition
and Bio-efficacy of Acha (Digitaria exilis and Digitaria iburua) and Soybean (Glycine
max) Based Complementary Foods in Rats. Annals. Food Science and Technology, 20(1):
171-182. www.afst.valahia.ro
Varley, H., Gewenlock, A. and Bell, M. (1980). Practical Clinical Biochemistry, 1. 5th ed., 741,
London: William Heinemen Medical. Books, Ltd.
Wadmare VB, Gadhe KS and Joshi MM (2019). Studies on physical and chemical composition of
Broccoli (Brassica oleracea L.). International Journal of Chemical Studies 2019; 7(2): 825-
828
Wieland, H. and Seidel, D. (1983). A fully enzymatic colorimetric determination of HDL-
cholesterol in the serum. Journal of Nutrition, 109: 760-766.