IIARD International Institute of Academic Research and Development

JOURNAL OF BIOLOGY AND GENETIC RESEARCH (JBGR )

E-ISSN 2545-5710
P-ISSN 2695-222X
VOL. 10 NO. 2 2024
DOI: 10.56201/jbgr.v10.no2.2024.pg22.37

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Effects of Portulaca oleracea Stem Extract on Male Reproduction in Albino Rats ( Rattus norvegicus )

Wobo, Ekemini Macaulay

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Abstract

Portulaca oleracea (Purselane) is a green leafy vegetable that is said to have some health promoting nutrients , however data on the safety and toxicology profile are scarce hence this study. The aim of this study was to evaluate the reproductive effect and toxicological profile of methanol stem extracts of Portulaca oleracea using albino rats as model. Fresh stem of Portulaca oleracea were collected and dried at room temperature to constant weight over 6 weeks, the dry stem weighed and grounded to fine powder. Methanol solvent (1.5L) was used and successive solvent extraction by cold maceration was done for 72hours. It was filtered with a filter paper and filtrate obtained, concentrated with rotary evaporator, transferred to evaporating dish and dried over a water bath. 82 albino rats were used: 12 for the acute toxicity study and 64 for the experimental study proper. Animals were acclimatized for 2 weeks before the study. In the experiment proper, animals were divided into four groups of 16 animals each. Group A: (control) received 1.5mb of clean water, Group B: 125mg/kg body weight of extract, Group C: 250mg/kg of extract, Group

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