IIARD International Institute of Academic Research and Development

International Journal of Agriculture and Earth Science (IJAES )

E- ISSN 2489-0081
P- ISSN 2695-1894
VOL. 10 NO. 6 2024
DOI: 10.56201/ijaes.v10.no6.2024.pg136.148

---

Histological and Histochemical Study of Thyroid Gland in Bull (Bos Taurus) in Al Muthanna province

Khalid thamer hashee and Bassim Abdullah Jassim Al-khuzae :

---

Abstract

The current study aimed to finding of the histological and histochemical characteristics of the thyroid glands in adult males of bull . Fifteen adult male bull to was used in this study. Histological study was used to describe the general structure of thyroid, in addition to microscopic measurements included the thickness of capsule, diameter of different thyroid follicles. According to histological findings, the thyroid glands in each animal had a capsule covering them that was made up of two layers of collagen fibers. Thyroid gland follicles in three different shapes and sizes made up every lobe of the thyroid gland: large, medium, and small follicles . The histological result revealed the Para follicular cells in the bull, also referred to as C-cells. C-cells are large, spherical, and oval in shape, and their cytoplasm was stained lighter than that of follicular cells. Their nuclei were also densely stained. They began as a single cell or in groups of two or three. In the tissue section, it was observed that certain C-cells were supported by a basement membrane, serving as the bases of both follicular and Para follicular cells. In contrast, other cells were arranged haphazardly in the intermolecular tissue, separating the Ccells from the follicular wall. The Para follicular cells, which were observed in tissue sections either singly or in small groups, have an oval or rounded shape, a spherical nucleus, and light stain cytoplasm . Histochemical results showed that the capsule, colloid material, basement membrane and Para follicular cells of thyroid gland showed intense reactions. To PAS stain All parts of thyroid l glands was exhibited positive reaction to Alcian blue_ PAS and Masson Trachoma stain .

keywords:

Histochemical, positive and Alcian blue_ PAS

---

References:

Rajathi S (2019) Ultrasound Anatomy of the Thyroid Gland in Dogs. J Anim Res 9:
10.30954/2277-940x.04.2019.5.
Peksa, Z., Trávnícek, J., Dusová, H., Konecný, R., & Hasonová, L. (2011). Morphological
and histometric parameters of the thyroid gland in slaughter cattle. Journal of
Agrobiology, 28(1), 79.
Alwan A F. 2009. Sheep fetal thyroid development, with adult plasma T4 and T3 hormones
concentrations. Journal of Animal and Veterinary Advances 11: (2) 115–
Solaiman, S. G. (Ed.). (2010). Goat science and production. John Wiley & Sons.

Yousef FA, Karima GMM, Mohamed MMK, Nawito MF, AbdelRazik AMA (2016)
Histomorphometry aspect of thyroid gland and biochemical profile in pregnant and
nonpregnant dromedary camels. Afr J Biotechnol 15: 370-5

Hussein, B. M., Walaa, F. O., & Dawood, G. A. (2023) . A Review of Anatomical and
Histological Features of the Thyroid Gland In Different Species of Animals. Diyala
Journal for Veterinary Sciences, 1(3), 72-83
Wang, Y., Wu, L. P., Fu, J., Lv, H. J., Guan, X. Y., Xu, L., ... & Shi, B. Y. (2013).
Hyperthyroid monkeys: a nonhuman primate model of experimental Graves’ disease. J
Endocrinol, 219(3), 183-193.

Kausar, R. and Shahid, R. V. (2006). Gross and microscopic anatomy of the thyroid gland of
the one-humped camel (Camelusdromedarius). Pakistan Vet. J., 26, Pp

Olukole, S. G; Adeagbo, M. A and Oke, B. O. (2016). Histology and Histochemistry of the
Adrenal Gland African Giant Rat (Cricetomys gambianus, Waterhouse), Int. J. Morphol.,
34(4):1455-1460.

Pankowski, F., Bartyzel, B. J., Pa?ko, S., Moroz, A., Mickiewicz, M., Szalu?-Jordanow, O.,
& Bonecka, J. (2021). CT appearance and measurements of the normal thyroid gland in
goats. BMC Veterinary Research, 17, 1-8.

Skripkin, V., Kvochko, A., Derezina, T., Kuzminova, A., Cymbal, I., Belugin, N., &
Pisarenko, N. (2019, December). Dynamics of thyroid hormones in Stavropol breed
sheep in postnatal ontogenesis. In IOP Conference Series: Earth and Environmental
Science (Vol. 403, No. 1, p. 012064). IOP Publishing.

Hussin, A.M and Al-Taay, M. M. (2009). Histological study of the thyroid and parathyroid
glands in iraqi buffalo bubalus bubalis with referring to the seasonal changes.
Bas.J.Vet.Res.8 (1) :26-38.

Virili, C., Fallahi, P., Antonelli, A., Benvenga, S., & Centanni, M. (2018). Gut microbiota
and Hashimoto’s thyroiditis. Reviews in Endocrine and Metabolic Disorders, 19, 293-
Yaglova, N. V., & Yaglov, V. V. (2017). Cytophysiological changes in the follicular
epithelium of the thyroid gland after long-term exposure to low doses of
dichlorodiphenyltrichloroethane (DDT). Bulletin of experimental biology and
medicine, 162(5), 699-702.
Yousef FA, Karima GMM, Mohamed MMK, Nawito MF, AbdelRazik AMA (2016)
Histomorphometry aspect of thyroid gland and biochemical profile in pregnant and
nonpregnant dromedary camels. Afr J Biotechnol 15: 370-5

Enemali, F.U; Hambolu, J. O; Alawa, J. N and Anosike, I. V. (2016).Gross anatomical,
Histological and Histochemical studies of thyroid gland of African Giant Rat
(Cricetomys gambianus-water house). Journal of Pharmacy and Biological
Sciences, 11(4):40-43.

Igbokwe C.O. (2010). Gross and Micoscopic Anatomy of Thyroid Gland of the wild
African Grass cutter in Southern Nigerian, Enviromental Journal of Anatomy 14
(1): 5-10.

Sekulic, M; Sosic-jurjevic,B;Filipovic,B;Nestorovic,N; Negic,N ;Stojanoski,M.M and
Milosvic, M. (2007). Effect of Estradiol and Progesterone on Thyroid Gland in Pigs: A
Histochemical, Stereological, and Ultrastructural Study. Microscopy Research and
Technique 70:44–49.
Mense, M. G., & Boorman, G. A. (2018). Thyroid gland. In Boorman's pathology of the rat
(pp. 669-686). Academic Press.

Abdul-Hamid, M; and Salah, M. (2013). Lycopene reduces deltamethrin effects induced
thyroid toxicity and DNA damage in albino rats. The Journal of Basic & Applied
Zoology, 66(4):155-163.

Maaruf, N. A., Mahmood, Z. M., & Amen, P. J. M. (2017). Effect of Aspartame on the
Rat’s Thyroid Gland: A histological and Morphometrical Study.

Nilsson, M., & Fagman, H. (2017). Development of the thyroid gland. Development,
144(12), 2123-2140.Mc Donald, L.E. Veterinary Endocrinology and Reproduction. 3rd
ed. Lea and Fibger, Philadelphia, 1980.

Alturkistani HA, Tashkandi FM, Mohammedsaleh ZM (2015).Histological Stains: A
Literature Review and Case Study. Global J Healt Sci 8: 72.

Prasanth, B. A. ; Jagapathi, R. P.; Patki, H. S. and Chandrasekhara, R. T.S. (2012).
Histology of the Thyroid and Parathyroid Glands of Cat. Indian Vet. J., 89 (9) Pp: 84 –
Rejeb, A; Amara, A; Rekik, M; Rezeigui, H., and Crespeau, F. (2011).
Histomorphometry and hormone secretion of the thyroid gland of the dromedary
(Camelus dromedarius). Journal of Camelid Science, 4, 10-22.

DOWNLOAD PDF

---

Back