IIARD International Institute of Academic Research and Development

JOURNAL OF BIOLOGY AND GENETIC RESEARCH (JBGR )

E-ISSN 2545-5710
P-ISSN 2695-222X
VOL. 10 NO. 1 2024
DOI: 10.56201/jbgr.v10.no1.2024.pg42.52

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Isolation And Identification of Bacteria from Infected Burns with Pathological Changes and Evaluation the Antibacterial Effects of Lactobacillus Rhamnosus and Lactobacillus Buchnerri Filtrates

Noora A. Hassan

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Abstract

This study was done for evaluation the antimicrobial effects of Lactobacillus rhamnosus and Lactobacillus buchnerri filtrates on the main causes of burn infections with studying the histopathology of this burn. One hundred thirty-five swabs were taken from 135 burn cases from patients come to clinics in Baghdad. These swabs were cultured on primary media, then biochemical tests were done for identification of these bacteria. Lactobacillus filtrates was prepared. The antimicrobial susceptibility test for this filtrate was done by using agar well diffusion, also antibiotic susceptibility test was done for many antibiotics against bacterial isolates. The slices revealed the epithelium layer, the top layer of skin, and the dermal layer, the middle layer, both of which were destroyed. The presence of bacteria is confirmed by the significant infiltration of leukocytes. This was consistent with what was seen visually, namely the ulceration of the burn region and the presence of pus. The current results showed that Pseudomonas aeruginosa was the most prevalent isolates from infected burns followed by E. coli. The results of antibiotic sensitivity test showed that most isolates were MDR to most antibiotics, While the Lactobacillus filtrates showed a high antimicrobial effectivity against all bacteria. In conclusion, Pseudomonas aeruginosa was most predominant bacteria isolated from burns, also other bacteria were isolated in significant percentages which showed high susceptibility to lactobacillus filtrates.

keywords:

Lactobacillus, Burns, Bacteria

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