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Phytoconstituents, Antioxidant Potential and Antiulcer Activity of Ficus Exasperata

Mabel E. Oderoha, Osaro Iyekowa, Kennedy O. Ogbeide, Osaze Edosuyi, Kester C., Chukwuemeke, Amuche A. Goddy, Osayemwenre Erharuyi

Abstract

Medicinal plants continue to play an essential role in global healthcare and drug discovery. According to the world health organization, nearly 80% of the world Population depend on plant-based medicine for primary healthcare Plants contain numerous bioactive phytochemicals-flavonoids, alkaloids, tannins, saponins and phenols-that promote mucosal protection, inhibit acid secretion and facilitate ulcer healing. This study investigated the phytochemical, antioxidant activity, and antiulcer potential of ficus exaspereta leaves. The phytochemical constituents were determined by standard methods, the antioxidant activity was ascertained by the DPPH assay, while the antiulcer potential was assessed by an ethanol-gastric induced model in wister rats. Bioactive constituents identified in the methanol extract included alkaloids, flavonoids, glycoside, phenols and tannins. The extract exhibited a total phenolic (29.5mgGAE/g) and flavonoid (122.8mg QE/g) contents, contributing to a strong antioxidant activity in the DPPH assay, with an IC50 of 1.90μg/mL, compared to 1.30 μg/mL for ascorbic acid. The extract demonstrated significant gastroprotection with the 200mg/kg dose showing the highest ulcer inhibition (44.10%). These finding show that Ficus exasperata possesses promising anti-ulcer properties at moderate dose supporting its ethnomedicinal uses in managing gastric ulcers.

Keywords

Ficus exasperataPhytochemicalUlcerAntioxidant.

References

Abbaspour, N., Hurrell, R., and Kelishadi, R. (2014). Review on iron and its importance for human health. Journal of Research in Medical Sciences, 19(2):164–174. Akar, Z., Küçük, M., Doğan, H. (2017). A new colorimetric DPPH scavenging activity method with no need for a spectrophotometer applied on synthetic and natural antioxidants and medicinal herbs. J Enzyme Inhib Med Chem., 32(1):640–7. Akwukwaegbu P. I., Peters D. E., Wegwu M. O. (2016). Proximate Analysis and Phytochemical Screening of Fluted Pumpkin ( Telfairia occidentalis) Pod. American Journal of Food, Nutrition and Health, 1(1): 1-6 Anyanwu, B.C., Akoh, O.U. & Otuokere, I.E. (2022). Phytochemical screening and proximate analysis of the leaves of Launaea (Lactuca) taraxacifolia. Journal of Chemical Society of Nigeria, 47(2), pp.421–432. Arab, H.H., Salama, S.A., Omar, H.A., Arafa, E.S.A., Maghrabi, I.A. (2015). Diosmin Protects against Ethanol-Induced Gastric Injury in Rats. Novel Anti-Ulcer Actions, 1:10. Baba, H.S. and Mohammed, M.I. (2021). Determination of some essential metals in selected medicinal plants’, ChemSearch Journal, 12(1):15–20. Begum, H.A., Hamayun, M., Shad, N., Yaseen, T. and Asad, F. (2018). Nutritional analysis of some selected medicinal plants of Khyber Pakhtunkhwa, Pakistan. Pure and Applied Biology, 7(3):955–964. https://doi.org/10.19045/bspab.2018.700114 Bhattacharya, S. A., Maity, S. U., Pramanick, D. E., Hazra, A. K. & Choudhury, M. A. (2016). HPLC of phenolic compounds, antioxidant and antimicrobial activity of bulbs from three Ornithogalum species available in India. Int. J. Pharm. Pharm. Sci. 8:187– 192. Giacco, R., Costabile, G., & Riccardi, G. (2016). Metabolic effects of dietary carbohydrates: The importance of food digestion. Food Research International, 88:336–341. doi:10.1016/j.foodres.2015.10.026. Guo, W., Nazim, H., Liang, Z., & Yang, D. (2016). Magnesium deficiency in plants: An urgent problem. The Crop Journal, 4(2):83 -91. https://doi.org/10.1016/j.cj.2015.11.00 Wang, M., Zheng, Q., Shen, Q. and Guo, S. (2013). The critical role of potassium in plant stress response’, International Journal of Molecular Sciences, 14(4):7370–7390. doi:10.3390/ijms14047370. Heinrich, M., Mah, J., and Amirkia, V. (2021). Alkaloids used as medicines: Structural phytochemistry meets biodiversity— an update and forward look. Molecules, 26(7):1836. doi:10.3390/molecules26071836. Hernandez-Munoz, R., Montiel-Ruiz, C., Vazquez-Martinez, O. (2000). Gastric Mucosal Cell Proliferation in Ethanol-Induced Chronic Mucosal Injury is Related to Oxidative Stress and Lipid Peroxidation in rats. Laboratory Investigation, 80(8):1161–1169. Isong, E. U. and Idiong, U. I. (1997). Comparative studies on the nutritional and toxic composition of three varieties of Lesianthera africana. Plant Foods for Human Nutrition, 51: 79-84 Udosen, E. O., Udok, U. E. and Unuigue, O. S. (1998). The comparison of the Nutrient composition of Lasianthera africana and Heinsia crinata. J. Food Biochem., 23:571- 576 Effiong, G. S., Ogban, P. I., Ibia, T. O., & Adam, A. A. (2009). Evaluation of Nutrient- supplying Potentials of Fluted Pumpkin (Telfairia occidentalis, Hook, F.) And Okra (Abelmoschus esculentus) (L.) Moench. Academic Journal of Plant Sciences 2 (3): 209-214 IJCCP , Jaglan, P., Kaushik, D., Kumar, M., Kumar, A., Kaur, J., Oz, E., Brennan, C., Proestos, C., Brennan, M., Ahmad, N., Elobeid, T. and Oz, F. (2023). Structural, Thermal,Techno- Functional and Chemical Characterization Using FourierTransform Infrared Spectroscopy, Gas- Chromatography-MassSpectrophotometry, Thermogravimetric Analyser, Field EmissionScanning Electron Microscopy and Energy-Dispersive X- raySpectrometer of Moringa Oleifera Flower Powder. InternationalJournal of Food Science & Technology 58, 11: 5992–6005 Kaur, J., Kaushik, D., Kumar, M., Babagil, G.E., Amarowicz, R., Proestos, C., Oz, E., Oz, F. and Esatbeyoglu, T., (2025). Comprehensive analysis and characterization of Mangifera indica L. leaf powder. Food Science & Nutrition, 13:e70083. Kuna, L., Jakab, J., Smolic, R., Raguz-Lucic, N., Vcev, A., & Smolic, M. (2019). Peptic Ulcer Disease: A Brief Review of Conventional Therapy and Herbal Treatment Options. Journal of Clinical Medicine, 8(2):179. doi:10.3390/jcm8020179 Pandey, K.B., and Rizvi, S.I. (2009). Plant polyphenols as dietary antioxidants in human health and disease. Oxidative Medicine and Cellular Longevity, 2(5):270–278. doi:10.4161/oxim.2.5.9498. Princwill- Ogbonna, I. L., P. C. Ogbonna, and I. B. Ogujiofor. (2019). Proximate Composition, Vitamin, Mineral and Biologically ActiveCompounds Levels in Leaves of Mangifera indica , Perseaamericana (Avocado Pea), and Annona muricata (Sour Sop). Journal of Applied Sciences and Environmental Management 23(1): 65– 74. Rahman, M.M., Islam, M.B., Biswas, M., and Alam, A. H. M. K. (2015).In vitro antioxidant and free radical scavenging activity of different parts of Tabebuia pallida growing in Bangladesh. BMC Res Notes, 8(1):621. Rodríguez-Rodríguez, E., Navia Lombán, B., López-Sobaler, A.M. and Ortega Anta, R.M. (2010). Review and future perspectives on recommended calcium intake. Nutrición Hospitalaria, 25(3):366–374. Sener, G., Pasakalogu, K., Ayanoglu-Dulger, G. (2004). Protective Effect of Increasing Doses of Famotidine, Omeprazole, Lansoprazole, and Melatonin against Ethanol-Induced Gastric Damage in Rats. Indian Journal of Pharmacology, 36:171-174. Shawon, L., Gautam, P. (2012). An Overview of the Current Methodologies Used for Evaluation of Gastric and Duodenal Anti-Ulcer Agents. Pharmacologia, 3(8):249– 257. Silva, A. P. S. da, Nascimento da Silva, L. C., Martins da Fonseca, C. S., de Araújo, J. M., Correia, M. T. dos S., Cavalcanti, M. da S., & Lima, V. L. de M. (2016). Antimicrobial Activity and Phytochemical Analysis of Organic Extracts from Cleome spinosa Jaqc. Frontiers in Microbiology, 28:7:963 doi:10.3389/fmicb.2016.00963 Škrovánková, S., Mišurcová, L., & Machů, L. (2012). Antioxidant Activity and Protecting Health Effects of Common Medicinal Plants. Advances in Food and Nutrition Research, 75–139. doi:10.1016/b978-0-12-394598-3.00003-4 Sreelatha, S., Padma, P.R. (2009). Antioxidant activity and total phenolic content of Moringa oleifera leaves in two stages of maturity. Plant Foods Hum Nutr., 64(4):303–11. Takagi, K., Okabe, S., Saziki, R. (1970). A New Method for the Production of Chronic Gastric Ulcers in Rats and the Effect of Several Drugs on Its Healing. Japanese Journal of Pharmacology, 19:418-421. Tariq, S., Umbreen, H., Noreen, R., Petitbois, C., Aftab, K., Alasmary, F. A. and Mazid, M. A. (2022). Comparative Analysis of Antioxidants Activity of Indigenously Produced Moringa Oleifera Seeds Extracts. Biomed Res Int. 2022:4987929. IJCCP , Thilagavathi, T. , Pandiyan M., Suganyadevi M., Sivaji M., Yuvaraj M., and Sasmitha R.. (2020). “Dietary Fibre‐Health Benefits.” Biotica Research Today 2, no. 6: 519–522. Tiwari, P., Kumar, B., Kaur, M., Kaur, G., Kaur, H. (2011). Phytochemical screening and extraction: a review. Internationale Pharmaceuticasciencia, 1(1):98–106. Tlili, N., Sarikurkcu, C. (2020). Bioactive compounds profile, enzyme inhibitory and antioxidant activities of water extracts from five selected medicinal plants. Ind. Crops Prod. 151:112448 https://doi.org/10.1016/j.indcrop.2020.112448. Trinh, P-C., Thao, L-T-T., Ha, H-T-V. & Nguyen, T-A. (2020). DPPH-scavenging and antimicrobial activities of Asteraceae medicinal plants on uropathogenic bacteria. Evidence-Based Complementary and Alternative Medicine, Article ID 7807026, 9 pages. https://doi.org/10.1155/2020/7807026 Ullah, S., Munir, S., Badshah, S. L., Khan, N., Ghani, L., Poulson, B. G., ... Jaremko, M. (2020). Important flavonoids and their role as a therapeutic agent. Molecules, 25(22):5243. doi:10.3390/molecules25225243 Umar, A., Jimoh, W.L.O. & Garba, M.D. (2011). Proximate analysis of Lepidium sativum leaves. Chemsearch Journal, 2(1):26–27. Usunobun, U., Okolie, N. P., Anyanwu, O. G., and Adegbegi, A.J. (2015). Phytochemical Screening and ProximateComposition of Annona muricata Leaves. European Journal of BotanyPlant Science and Phytology 2(1): 18–28. Yusiharni, E., & Gilkes, R. (2012). Minerals in the ash of Australian native plants. Geoderma, 189-190, 369–380. doi:10.1016/j.geoderma.2012.06.035 Zohra, S.F., Meriem, B., Samira, S., Muneer, M.A. (2012). Phytochemical screening and identification of some compounds from mallow. J Nat Prod Plant Resour, 2(4):512– 516.