Submit your papersSubmit Now
For Enquiries: [email protected]
IIARD LogoIIARD

Bioremediation of Hydrocarbon-Polluted Soils in the Niger Delta: A Comparative Review of Remediation Techniques and Biostimulation Products, With Emphasis on Clogen and Solpawa: A Comparative Review

Ukpe Aniefiok., E, Edmund Nkwocha, E., Emereibeole Enos, I., Mgbeahuruike, Leonard, U

Abstract

Petroleum exploration, production and transportation activities in Nigeria's Niger Delta have generated an extensive legacy of hydrocarbon-contaminated soils that threaten soil fertility, plant growth, groundwater quality and human health. Numerous physical, chemical and biological techniques have been developed to remediate such soils, but bioremediation, and biostimulation in particular, has emerged as the most economical and environmentally compatible strategy for the large, diffuse contamination that characterises the region. This review compares the principal bioremediation strategies documented in the literature, namely biostimulation, bioaugmentation, phytoremediation and mycoremediation, in terms of mechanism of action, reported total petroleum hydrocarbon degradation efficiency and operational constraints, and undertakes a focused comparative analysis of two indigenously manufactured Nigerian biostimulation products, Clogen and Solpawa, that are increasingly deployed by remediation contractors across the Niger Delta. Drawing on published field and laboratory studies, the review shows that although both products can independently achieve TPH degradation efficiencies exceeding 90%, they act through complementary rather than duplicate mechanisms: Clogen through soil conditioning, hydrocarbon emulsification and bioaugmentation, and Solpawa through concentrated nitrogen-phosphorus- potassium nutrient supplementation. This complementarity suggests that a combined application strategy may deliver more consistent and accelerated remediation outcomes than either product used in isolation. The review identifies the absence of published data on combined Clogen- Solpawa application as a significant research gap and outlines factors likely to govern the success of such a combined regime under Niger Delta field conditions.

Keywords

bioremediation; biostimulation; hydrocarbon-polluted soil; Clogen; Solpawa; Niger Delta; total petroleum hydrocarbon degradation.

References

Abdel-Shafy Hussein I, Mansour Mona SM. A review on polycyclic aromatic hydrocarbons: Source, environmental impact, effect on human health and remediation. Egyptian Journal of Petroleum. 2016;25:107-123. DOI: 10.1016/j.ejpe.2015.03.011 Abdulsalam, S., Bugaje, I., Adefila, S., & Ibrahim, S. (2011). Comparison of biostimulation and bioaugmentation for remediation of soil contaminated with spent motor oil. International Journal of Environmental Science & Technology, 8(1), 187-194. https://doi.org/10.1007/BF03326208 AbouSamra RM, Ali R (2022). Monitoring of oil spill in the offshore zone of the Niger Delta using Sentinel data. Marine Pollution Bulletin, 179:113718. Adams, G. O., Fufeyin, P. T., Okoro, S. E., &Ehinomen, I. (2015). Bioremediation, biostimulation and bioaugmention: A review. International Journal of Environmental Bioremediation & Biodegradation, 3(1), 28-39. Ahmad A, Mustafa G, Rana A, Zia AR. (2023).Improvements in Bioremediation Agents and Their Modified Strains in Mediating Environmental Pollution. CurrMicrobiol. 2023;80(6):208. doi: 10.1007/s00284-023-03316-x Aislabie, J., McLeod, M., and Fraser, R. (2018). 'Potential for Biodegradation of Hydrocarbons in Soil from the Ross Dependency, Antarctica.' Applied Microbiology and Biotechnology 49: 210-214. Al-Kindi, S., and Abed, R. M. M. (2016). Comparing oil degradation efficiency andbacterialcommunitiesincontaminatedsoilssubjectedtobio stimulationusing different organic wastes. Water. Air. Soil Pollut. 227 227, 36. doi:10.1007/s11270015-2722-x Albert,O.N.,Amaratunga,D.,&Haigh,R.P.(2018).Evaluationoftheimpacts of oil spill disaster on communities and its influence on restiveness in Niger Delta, Nigeria. Procedia Engineering, 212, 1054--1061. https://doi. org/10.1016/j.proeng.2018.01.136 Alegbeleye OO, Opeolu BO, Jackson V.(2017). Bioremediation of polycyclic aromatic hydrocarbon compounds: (acenaphthene and fluorene) in water using indigenous bacterial species isolated from the Diep and Plankenburg rivers, Western Cape, South Africa. BJM. 2017;48(2):314-325. doi: 10.1016/j.bjm.2016.07.027 30. Ameen F, Moslem M, Hadi S, Al-Sabri AE. Biodegradation of diesel fuel hydrocarbons by mangrove fungi from Red Sea Coast of Saudi Arabia. Saudi Journal of Biological Sciences. 2016;23:211-218. DOI: 10.1016/j. sjbs.2015.04.005 Arocena, J. M. and Rutherford, P. M. (2015). Properties of hydrocarbonand salt-contaminated flare pit soils in northeastern British Columbia . Chemosphere, 60(4): 567--575. Asemoloye, M. D., Ahmad, R., and Jonathan, S. G. (2017). Synergistic action of rhizosphericfungiwithMegathyrsusmaximusrootspeedsuphydrocarbondegradation kinetics in oil polluted soil. Chemosphere 187, 1--10. doi:10.1016/j.chemosphere.2017. 07.158 Atonye JJ (2023). Acute health effects of crude oil spill in a rural community in Bonny, Rivers State, Nigeria. Cent Asian Journal of Medicine and Natural Sciences. 4 (2): 244-259. Azubuike CC, Chikere CB, Okpokwasili GC. (2016).Bioremediation techniques-classification based on site of application: Principles, advantages, limitations and prospects. World Journal of Microbiology and Biotechnology. 2016;32:180. DOI: 10.1007/s11274- 016- 2137-x Badejo, O.T. and Nwilo, P.C., (2014). Management of Oil Spill Dispersal Along the Nigerian Coastal Areas. ISPRS Congress, Istanbul, Turkey. Bala, S., Garg, D., Thirumalesh, B. V., Sharma, M., Sridhar, K., Inbaraj, B. S., et al. (2022).Recentstrategiesforbioremediationofemergingpollutants:areviewforagreen and sustainable environment. Toxics 10, 484. doi:10.3390/toxics10080484 Bastida F, Jehmlich N, Lima K, Morris BEL, Richnow HH, Hernández T, et al. The ecological and physiological responses of the microbial community from a semiarid soil to hydrocarbon contamination and its bioremediation using compost amendment. Journal of Proteomics. 2016;135:162-169. DOI: 10.1016/j.jprot.2015.07.023 Bezza FA, NkhalambayausiChirwa EM. Biosurfactant from Paenibacillusdendritiformis and its application in assisting polycyclic aromatic hydrocarbon and motor oil sludge removal from contaminated soil and sand media. Process Safety and Environmental Protection. 2015;98:354364. DOI: 10.1016/j.psep.2015.09.004 Bicca, F.C., Fleck, L.C. and Ayub, M.A.Z. (2019): Production of biosurfactant by hydrocarbon degrading Rhodococcus rubber and Rhodococcuserythropolis. Revista de Microbiologia 30, 31-37. Chaudhary, D. K., Bajagain, R., Woo, S., and Jaisoo, J. (2021). Effect of consortium bioaugmentationandbiostimulationonremediationefficiencyandbacterialdiversityof diesel- contaminated aged soil. World J. Microbiol. Biotechnol. 37, 1--12. doi:10.1007/ s11274- 021-02999-3 Chaudhary, D.K.,andKim,J.(2019). New insights into bioremediationstrategiesforoil- contaminatedsoilincoldenvironments.Int.Biodeterior. Biodegr.142,58--72.doi:10. 1016/j.ibiod.2019.05.001 Chettri, B., Singha, N. A., and Singh, A. K. (2021). Efficiency and kinetics of Assam crude oil degradation by Pseudomonasaeruginosa andBacillussp.Arch.Microbiol.203, 5793--5803. doi:10.1007/s00203-021-02567-1 Das, N., and Chandran, P. (2011). Microbial degradation of petroleum hydrocarbon contaminants: an overview. Microb. Degrad. Petroleum Hydrocarbon Contam. Overv. Biotechnol. Res. Int. 2011, 1--13. doi:10.4061/2011/941810 Daâssi, D., and Almaghribi, F. Q. (2021). Soil microcosms for bioaugmentation with fungal isolates to remediate petroleum hydrocarbon-contaminated soil. Preprint,1--38. doi:10.21203/rs.3.rs-1086969/v1 Dhailappan, A., Arumugam, A., Rajendran, G., and Ravichandran, M. (2022). A dualisticapproachtoinvestigatetheremedialpotentialandediblepropertyofPleurotus ostreatusonhydrocarbon-contaminatedsoil.J.Appl.Biol.Biotechnol.10,70--78.doi:10. 7324/jabb.2022.100310 Dindar, E., Olcay, F., Sagban, T., and Baskaya, H. S. (2013). Bioremediation of petroleum- contaminated soil petrol ilekirlenmiştopraklarınbiyoremediasyonu. J. Biol. Environ. Sci. 7, 39--47. doi:10.3390/ijms19113373 Eggen, T., and Šašek, V. (2012). 'Use of Edible and Medicinal Oyster Mushroom (Pleurotusostreatus (Jacq.: Fr.) Kumm.) Spent Compost in Remediation of Chemically Polluted Soil.' International Journal of Medicinal Mushrooms 4 (3): 255-261. Ekundayo, E. and Obuekwe, O. (2020). Effects of an oil spill on soil physico-chemical properties of a spill site in a typicudipsamment of the Niger delta basin of Nigeria. Environmental Monitoring and Assessment, 60(2): 235-249. Falkova M, Vakh C, Shishov A, Zubakina E, Moskvin A, Moskvin L, et al. Automated IR determination of petroleum products in water based on sequential injection analysis. Talanta. 2016;148:661-665. DOI: 10.1016/j. talanta.2015.05.043 Fanaei F, Moussavi G, Shekoohiyan S.(2023). Enhanced bioremediation of oil-contaminated soil in a slurry bioreactor by H2O2-stimulation of oil-degrading/ biosurfactant-generating bacteria: performance optimization and bacterial metagenomics. Biodegradation. 2023;34(1):83-101. doi: 10.1007/ s10532-022-10008-z Gaur,V. K., Gupta,S.,andPandey,A. (2021).Current trends and research In Industrial Wastewater Treatment Through Evolution in mitigation approaches for petroleum oil-polluted environment: recent advances and future directions.Biotechnology. 2007;18:257-266. DOI: 10.1016/j.copbio.2007.04.006 Gogoi, B.K., Dutta, N.N. and Krishnamohn, T.R. (2013). A case study of bioremediation of petroleum-hydrocarbon contaminated soil at a crude oil spill site. Advances in Environmental Research ,7(4) :767-782. Gouda S, Kerry RG, Das G, Paramithiotis S, Shin H-S, Patra J. K.(2017). Revitalization of plant growth promoting rhizobacteria for sustainable development in agriculture. Microbiological Research. 2018;206:131140. DOI: 10.1016/j.micres.2017.08.016 Guarino, C., Spada, V., and Sciarrillo, R. (2017). Assessment of three approaches of bioremediation (Natural Attenuation, Landfarming and Bioagumentation -- assistitedLandfarming) for a petroleum hydrocarbons contaminated soil. Chemosphere 170, 10--16. doi:10.1016/j.chemo-sphere.2016.11.165 Hawksworth, D. L., and Lücking, R. (2017). Fungal diversity revisited: 2.2 to 3.8 million species. Fungal Kingd. 5, 79--95. doi:10.1128/9781555819583.ch4 Izah SC, Aigberua AO (2023). Potential threats and possible conservation strategies of biodiversity in Niger Delta Region in Nigeria. In: Sustainable utilization and conservation of Africa's biological resources and environment. Springer, 151-176. Jia J, Zong S, Hu L, Shi S, Zhai X, Wang B.(2017). The dynamic change of microbial communities in crude oil contaminated soils from oil fields in China. Soil and Sediment Contamination: An International Journal. 2017;26:171-183. DOI: 10.1080/15320383.2017.1264923 Kadafa, A. A. (2012). Environmental impact of oil exploration and exploitation in the Niger Delta. Global Journal of Science Frontier Research Environment and Earth Science, 12(3), 18- 28. \[Online\] Available: https.//:globaljournals.org Kebede, G., Tafese, T., Abda, E. M., Kamaraj, M., and Assefa, F. (2021). Factors influencing the bacterial bioremediation of hydrocarbon contaminants in the soil: mechanisms and impacts. J. Chem. 2021, 1--17. doi:10.1155/2021/9823362 Kisic, I., Mesic, S., Basic, F., Brkic, V., Mesic, M., Durn, G., Zgorelec, Z. and Bertovic, L. (2019). The effect of drilling fluids and crude oil on some chemical characteristics of soil and crops. Geoderma, 149(3--4): 209--216. Komommo, O. A., Ideriah, T. J. K., Gobo, A. E., & Egobueze, F. (2025). Bio-remediation of crude oil-contaminated soils in Eleme, Ogoni-land using CLOGEN, MicroSORB®, PRP® and a combination of Electro-Kinetic Action+KEEN®+Ors-SORB plus®. World Journal of Advanced Research and Reviews, 25(02), 142-154. https://doi.org/10.30574/wjarr.2025.25.2.0331 Koshlaf,E.,Shahsavari,E.,Haleyur,N.,MarkOsborn,A.,andBall,A.S.(2019).Effect of biostimulation on the distribution and composition of the microbial community of a polycyclic aromatic hydrocarbon-contaminated landfill soil during bioremediation. Geoderma 338, 216--225. doi:10.1016/j.geoderma.2018.12.001 Kristanti, R. A., Hadibarata, T., Toyama, T., Tanaka, Y., and Mori, K. (2011). Bioremediation of crude oil by white rot fungi Polyporus sp. S133. J. Microbiol. Biotechnol. 21, 995--1000. doi:10.4014/jmb.1105.05047 Kvenvolden, K. A., and Cooper, C. K. (2003). Natural seepage of crude oil into the marine environment. Geo-Marine Lett. 23, 140--146. doi:10.1007/s00367-003-0135-0 Lee YY, Lee SY, Cho KS.(2023).Long-term comparison of the performance of biostimulation and phytoextraction in soil contaminated with diesel and heavy metals. Chemosphere. 2023;337:139332. doi: 10.1016/j. chemosphere.2023.139332 Leonardi, V., Šašek, V., Petruccioli, M., D'Annibale, A., Erbanová, P., and Cajthaml, T. (2016). 'Bioavailability Modification and Fungal Biodegredation of PAHs in Aged Industrial Soils.' International Biodeterioration& Biodegradation 60: 165-170. Li, Y., Yang, J., Song, Y., & Wei, M. (2025). Progress in biostimulation-based remediation of TPH-contaminated soils: A comprehensive review. PeerJ, 13, e19991. https://doi.org/10.7717/peerj.19991 Lin, Q. and Mendelssohn, I. A. (2021). Impacts and recovery of the deepwater horizon oil spill on vegetative structure and function of coastal salt marsh in the northern Gulf of Mexico. Environmental Science and Technology, 46(7): 3737--3743. Macaulay, B., and Rees, D. (2014). Bioremediation of oil spills: a review of challenges for research advancement. Ann. Environ. Sci. 8, 9--37. Marín, F., Navarrete, H., and Narvaez-Trujillo, A. (2018). Total petroleum hydrocarbon degradation by endophytic fungi from the Ecuadorian Amazon. Adv. Microbiol. 08, 1029- -1053. doi:10.4236/aim.2018.812070 Mata OS, Flores MMA, Garcia BGU, Vazquez VA, Garcia EC.(2023).Villegas EAF. Bioremediation of Automotive Residual Oil-Contaminated Soils by Biostimulation with Enzymes, Surfactant, and Vermicompost. Int J Environ Res Public Health. 2023;20(16):6600. doi: 10.3390/ ijerph20166600 Mekonnen, B. A., Aragaw, T. A., & Genet, M. B. (2024). Bioremediation of petroleum hydrocarbon contaminated soil: A review on principles, degradation mechanisms, and advancements. Frontiers in Environmental Science, 12, 1354422. https://doi.org/10.3389/fenvs.2024.1354422 Morillo, E., Madrid, F., Lara-Moreno, A., and Villaverde, J. (2020). Soil bioremediation by cyclodextrins. A review. Int. J. Pharm. 591, 119943. doi:10.1016/j. ijpharm.2020.119943 NOSDRA. (2021). National Oil Spill Detection and Response Agency. https:// oilspillmonitor.ng/ Nardeli SM, Saad CF, de Barros Rossetto P, Caetano VS, Ribeiro-Alves M, Paes JES, et al. Transcriptional responses of Arabidopsis thaliana to oil contamination. Environmental and Experimental Botany. 2016;127:63-72. DOI: 10.1016/j. envexpbot.2016.03.007 Nassar, S. E., and Said, R. M. (2021). Bioremediation assessment, hematological, and biochemical responses of the earthworm (Allolobophoracaliginosa) in soil contaminated with crude oil. Environ. Sci. Pollut. Res. 28, 54565--54574. doi:10. 1007/s11356-021-13889-4 Nnadi VE, Udokporo EL, Okolo OJ (2022). Petroleum production activities and depletion of biodiversity: A case of oil spillage in the Niger Delta. In: Handbook of environmentally conscious manufacturing. Springer, pp 95-111. Nwankwegu, A. S., Onwosi, C. O., and Igbokwe, V. C. (2021). Bioremediation of petroleum- contaminated soil: A review of advances and limitations. Applied Microbiology and Biotechnology, 105(8), 3431-3446. Nzila A, Razzak SA, Zhu J.(2016)Bioaugmentation: An Emerging Strategy of Industrial Wastewater Treatment for Reuse and Discharge. Int J Environ Res Public Health. 2016;13(9):846. doi: 10.3390/ijerph13090846 Nzila A.(2018). Current Status of the Degradation of Aliphatic and Aromatic Petroleum Hydrocarbons by Thermophilic Microbes and Future Perspectives. Int. J. Environ. Res. Public Health 2018, 15, 2782; doi:10.3390/ijerph1512278 Okogbule-Wonodi, A., & Nweke, B. (2023). Influence of Solpawa SBR-XTM doses on petroleum hydrocarbon contaminants in sandy soil at different depths. European Journal of Science, Innovation and Technology, 3(5), 197-205. Okoro, C. C., Anidiobu, G. O., and Eze, S. O. (2020). Challenges in crude oil spill remediation: A comparative analysis of traditional and emerging technologies. Journal of Environmental Management, 256, 109921. Ordinioha, B., &Brisibe, S. (2013). The human health implications of crude oil spills in the Niger Delta, Nigeria: An interpretation of published studies. Journal of Nigeria Medical Association, 54(1), 10-16. https://doi.org/10.4103/0300-1652.108887 Popoola, L. T., Yusuff, A. S., Adeyi, A. A., and Omotara, O. O. (2022). Bioaugmentation and biostimulation of crude oil contaminated soil: process parameters influence. South Afr. J. Chem. Eng. 39, 12--18. doi:10.1016/j.sajce.2021. 10.003 Pratik Kakde and Jaigopal Sharma.(2024). Microbial Bioremediation of Petroleum Contaminated Soil: Structural Complexity, Degradation Dynamics and Advanced Remediation Techniques. J Pure ApplMicrobiol. 2024;18(4):2244-2261. https://doi.org/10.22207/JPAM.18.4.28 Qin,G.,Gong,D.,andFan,M.Y.(2013).Bioremediationofpetroleum-contaminated soil by biostimulation amended with biochar. Int. Biodeterior. Biodegr. 85, 150--155. doi:10.1016/j.ibiod.2013.07.004 Ramos-Vianna, M.M.G., Frano, J.H.R., Pinto, C.A., Valenzuela-Diaz F.R. and Buchler P.M., (2014).Sorption of oil pollution by organoclays and a coal mineral complex. Brazilian Journal of Chemical Engineering , 21(20): 239-295. Rhodes, C. J. (2014). Mycoremediation (bioremediation with fungi) - growing mushrooms to clean the earth. Chem. Speciat. Bioavailab. 26, 196--198. doi:10.3184/ 095422914X14047407349335 Roy, A., Dutta, A., Pal, S., Gupta, A., Sarkar, J., Chatterjee, A., et al. (2018). Biostimulation and bioaugmentation of native microbial community accelerated bioremediation of oil refinery sludge. Bioresour. Technol. 253, 22--32. doi:10.1016/j. biortech.2018.01.004 Sang-Hwan, l., Seokho, l., DaeYaeon, k. and Jeong- gyu, k. (2017). Degradation characteristics of waste lubricants under different nutrient condition. Journal of hazardous Materials, 143: 65-72. Sayara, T., and Sánchez, A. (2020). Bioremediation of PAH-contaminated soils: process enhancement through composting/compost. Appl. Sci. 10, 3684. doi:10.3390/ app10113684 Shi, L., Liu, Z., Yang, L., and Fan, W. (2022). Effect of plant waste addition as exogenous nutrients on microbial remediation of petroleum - contaminated soil. Ann. Microbiol. 72, 22. doi:10.1186/s13213-022-01679-3 Simmer RA, Schnoor JL. Phytoremediation, Bioaugmentation, and the Plant Microbiome. Environ Sci. and Tech. 2022;56(23):16602-16610. doi: 10.1021/ acs.est.2c05970 Smith E, Thavamani P, Ramadass K, Naidu R, Srivastava P, Megharaj M. Remediation trials for hydrocarboncontaminated soils in arid environments: Evaluation of bioslurry and biopiling techniques. International Biodeterioration and Biodegradation. 2015;101:56-65. DOI: 10.1016/j. ibiod.2015.03.029 Stepanova, A. Y., Gladkov, E. A., Osipova, E. S., Gladkova, O. V., and Tereshonok, D. V. (2022). Bioremediation of soil from petroleum contamination. Processes 10, 1224. doi:10.3390/pr10061224 Sutton, N. B., Maphosa, F., Morillo, J. A., Abu Al-Soud, W., Langenhoff, A.A., Grotenhuis, T., Rijnaarts, H.H. and Smidt, H. (2013). Impact of long-term diesel contamination on soil microbial community structure. Applied and Environmental Microbiology, 79(2): 619-- 630. Tolulope, A. O. (2014). Oil exploration and environmental degradation: the Nigerian experience. International Information Archives, International Society for Environmental Information Sciences, EIA04-039, 2, 387-393.H Torlapati, J., and Boufadel, M. C. (2014). Evaluation of the biodegradation of Alaska North Slope oil in microcosms using the biodegradationmodelBIOB. Front. Microbiol. 5 5, 212. doi:10.3389/fmicb.2014.00212 Townsend, G. T., Prince, R. C. and Suflita, J. M. (2013). Anaerobic oxidation of crude oil hydrocarbons by the resident microorganisms of a contaminated anoxic aquifer. Environmental Science and Technology, 37(22): 5213--5218. Tran HT, Lin C, Bui XT, et al. Aerobic composting remediation of petroleum hydrocarboncontaminated soil. Current and future perspectives. Sci Total Environ. 2021:753:142250. doi: 10.1016/j. scitotenv.2020.142250 UNEP. (2011). Environmental assessment of Ogoniland. (1--263). Nairobi: UNEP. https://doi.org/10.13140/RG.2.1.3307.1760 Udoette, U.B., (2017). Effects of Administration of Qua Iboe Light Crude Oil on Biochemical Ukoli, M.K. (2015). Environmental factors in the management of the oil and gas industry in Nigeria. www.cenbank.ng United Nation Development Program , (2006). Niger Delta Human Development Report 2006 , Abuja, Nigeria, pp. 19-73.

More Articles from RESEARCH JOURNAL OF PURE SCIENCE AND TECHNOLOGY

Advances In NLP-Driven Analytics for Automated Detection of Regulatory Liabilities in High-Volume Contracts

Author: Ngonadi Uchechi, Michael Ominyi, Ngozi Samuel Uzougbo, Blessing Chika Jones,, USA

Ambient Radiation Levels and Excess Lifetime Cancer Risk in Specialist Hospitals in Delta State, Nigeria

Author: E.E. Ono-Akporuke, E.O. Agbalagba, G.O. Avwiri and, V.B. Olaseni

Resilient Test Automation Framework for Microservices: Addressing Integration, Scalability, and Reliability

Author: Awonowo Olusegun Oriyomi,, Anrinnle Qowiu Olaoluwa, Lawal Ahmed Oladimeji, Babayemi Temitayo Matthew, Azubuike Marvelous Onyedikachukwu, Olomu Ayomide Babatunde, Omitogun Ayomide Elijah