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Antibiotic Susceptibility and Molecular Detection of Staphylococcus Aureus Isolated from Healthcare Facilities in Kano Metropolis

Baba A.M., Ahmed I., Shamsuddeen Umar, and Lambu Z.N., Corresponding author

Abstract

Staphylococcus aureus is one of the leading causes of healthcare-associated infections due to its ability to survive on hospital surfaces and develop resistance to multiple antimicrobial agents, particularly methicillin-resistant Staphylococcus aureus . This study investigated the prevalence, antibiotic susceptibility pattern, and molecular detection of Staphylococcus aureus isolated from hospital environments in selected healthcare facilities in Kano metropolis, Nigeria. A total of 286 environmental samples were collected from patients' bed rails, bed sheets, toilet door handles, and nurses' used hand gloves in three selected hospitals. Isolation and identification of bacterial isolates were carried out using standard cultural, Gram staining, and biochemical techniques. Antimicrobial susceptibility testing was performed using the Kirby–Bauer disc diffusion method in accordance with Clinical and Laboratory Standards Institute guidelines. Molecular confirmation of bacterial isolates was achieved using polymerase chain reaction amplification of species-specific genes. Of the 286 samples examined, 251 (87.76%) yielded bacterial growth, while 35 (12.23%) showed no growth. Staphylococcus aureus was the predominant isolate, accounting for 86 (30.06%) of all samples, followed by Escherichia coli 78 (27.27%), Klebsiella pneumoniae 63 (22.02%), and Pseudomonas aeruginosa 24 (8.39%). Nurses' used hand gloves recorded the highest level of S. aureus contamination. Antimicrobial susceptibility testing showed high resistance to Augmentin (69.8%), Ceftazidime (65.1%), Cefuroxime (65.1%), Gentamicin (60.5%), and Erythromycin (51.2%). Cefoxitin resistance was observed in 38 (44.2%) isolates, indicating a high prevalence of methicillin-resistant Staphylococcus aureus . PCR amplification successfully confirmed the molecular identity of the bacterial isolates through the detection of species-specific target genes.

Keywords

Staphylococcus aureushospital facilitiesantimicrobial susceptibilitymolecular detection

References

Abubakar, U., Yusuf, I., & Aliyu, S. (2023). Prevalence and antimicrobial resistance profile of methicillin-resistant Staphylococcus aureus in Nigerian healthcare facilities: A systematic review. African Journal of Clinical and Experimental Microbiology, 24(1), 1–15. Altschul, S. F., Gish, W., Miller, W., Myers, E. W., & Lipman, D. J. (1990). Basic local alignment search tool. Journal of Molecular Biology, 215(3), 403–410. Andrews, J.M. (2001). Determination of minimum inhibitory concentrations. Antimicrob. Chemother. 48(6):5-16 Centers for Disease Control and Prevention. (2019). Antibiotic resistance threats in the United States 2019. U.S. Department of Health and Human Services. Cheesbrough, M. (2014). District laboratory practice in tropical countries. (2nd edition), part two, University Press, Cambridge, UK. p. 47-54. Cheesbrough, M. (2018). District laboratory practice in tropical countries (3rd ed.). Cambridge University Press. Clinical and Laboratory Standards Institute. (2023). Performance standards for antimicrobial susceptibility testing (33rd ed., CLSI Supplement M100). CLSI. Dancer, S. J. (2014). Controlling hospital-acquired infection: Focus on the role of the environment and new technologies for decontamination. Clinical Microbiology Reviews, 27(4), 665– 690. David, L., Huber, W., and Granovskaia, M. (2006). Antimicrobial susceptibility testing protocols. Proc Natl Acad sci USA 103 (14): 5320-5 Ibrahim, S.M., Ahmad, I., Yahaya, S.M. and Ali, M. Isolation and Characterization of Staphylococcus Species from Clinical Samples Obtained from some Hospitals in Kano Metropolis, Nigeria. ARC Journal of Urology. 2019; 4(3): 1-6.doi:dx.doi.org/10.20431/ 2456-060X.0403001 Okesola, A. and Olola, A.F. (2011). The efficacy of the commonly used hospital disinfectants on Pseudomonas aeruginosa. International Research Journal of Microbiology (ISSN: 2141-5463) Vol. 2(7) pp. 226-229, August 2011. Otter, J. A., Yezli, S., Salkeld, J. A., & French, G. L. (2013). Evidence that contaminated surfaces contribute to the transmission of hospital pathogens and an overview of strategies to address contaminated surfaces in hospital settings. American Journal of Infection Control, 41(5 Suppl), S6–S11. Otter, J. A., and French, G. L. (2013). Molecular epidemiology of community-associated methicillin-resistant Staphylococcus aureus in Europe. The Lancet Infectious Diseases, 10(4), 227–239. Prestinaci, F., Pezzotti, P., & Pantosti, A. (2015). Antimicrobial resistance: A global multifaceted phenomenon. Pathogens and Global Health, 109(7), 309–318. Rawlinson, S., Ciric, L., & Cloutman-Green, E. (2019). How to carry out microbiological sampling of healthcare environment surfaces. Journal of Hospital Infection, 103(4), 363–374. Sambrook, J., & Russell, D. W. (2001). Molecular cloning: A laboratory manual (3rd ed.). Cold Spring Harbor Laboratory Press. Sanger, F., Nicklen, S., & Coulson, A. R. (1977). DNA sequencing with chain-terminating inhibitors. Proceedings of the National Academy of Sciences, 74(12), 5463–5467. Tamura, K., Stecher, G., & Kumar, S. (2021). MEGA11: Molecular Evolutionary Genetics Analysis version 11. Molecular Biology and Evolution, 38(7), 3022–3027). P-ISSN 2695-222X Tong, S. Y. C., Davis, J. S., Eichenberger, E., Holland, T. L., & Fowler, V. G. (2015). Staphylococcus aureus infections: epidemiology, pathophysiology, clinical manifestations, and management. Clinical Microbiology Reviews, 28(3), 603–661. World Health Organization. (2022). Global report on infection prevention and control. World Health Organization. World Health Organization. (2017). Global priority list of antibiotic-resistant bacteria to guide research, discovery, and development of new antibiotics. https://www.who.int/publications/i/item/WHO-EMP-IAU-2017.12

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