References
Abdullahi, N., Ariahu, C.C., and Abu, J.O. (2016). Effect of Chemical Hurdles on Sensory Attributes of Kilishi. Annals of Food Science and Technology 17(2). Chukwu, O. and Imodiboh, L. I. (2009). Influence of storage conditions on shelf-life of dried beef product (Kilishi). World Journal of Agricultural Sciences, 5 (1): 34-39. Cowan, S.T. (1985). Cowan and Steel’s Manual for Identification of Medical Bacteria, 2nd Edition, Cambridge University Press, Cambridge, London, 138-139. Dahiru, A.T., and Maigari, A.K. (2019). Bacteriological Quality Assessment of Kilishi Produced in Kunchi Local Government Area, Kano State, Nigeria.UMYU of the Journal of Microbiology Research.UJMR, 4 (1): 12 – 18. Ibrahim, T.N., Falake, O.O., and Galadima, M. (2021). Prevalence of Staphylococcus aureus and Escherichia coli in Sun-cured Meat (Jerky, Kilishi) from Retail Outlets in Sokoto, Nigeria. International Journal of Pharmaceutical Sciences and Clinical Research 1(1): 46-51. ICMSF (1996). International Commission on Microbiological Specifications for Food Microorganisms in Foods 5: Microbiological Specifications of Pathogens. p.89. Iheagwara, M.C., Chibuzo, I.H., Ibeawuchi, J.C., and Nzuruike, N.G. (2019). Physicochemical and Organoleptic characteristics of Kilishi as affected by meat types. Annals of Food Science and Technology, 20, 553-562. Iheagwara, M.C. and Okonkwo, T.M. (2016). Effect of Processing Techniques on the Microbiological Quality of Kilishi - A Traditional Nigerian Dried Beef Product. Journal of Meat Science and Technology: 4(1):11- 17.www.jakraya.com/journal/jmst. Inusa, S.K., and Muhammad, B.F. (2021). Evaluation of the Chemical Properties of Kilishi prepared from fermented cattle and camel beef in Semi-arid Nigeria. Nigeria Journal of Animal Production: 48(6): 88-97. Inusa, S.K., and Said, I.S. (2017). Evaluation of the Chemical and Microbiological Properties of Kilishi Sold in Kano Metropolis. Journal of Dryland Agriculture,3(1): 59 – 69. Iroha, I., Ugbo, EC., Ilang, D.C., Oji, A.E., and Ayogu, T.E. (2011). Bacterial contamination of raw meat sold in Abakaliki, Ebonyi State, Nigeria. Journal of Public Health and Epidemiology 3(2):49-53. Isah, O.A. and Okunbanjo, A.O. (2012). Effect of various additives on proximate composition and acceptability of Kilishi made from the semitendinosus muscle of white Fulani cattle. Pacific Journal of Science and Technology, 13(1): 506-511. Jabaka, R., Ododife, Q., Attah, D.D., and Nuhu, U.D. (2021). Assessment of Bacteria and Parasite Contamination of Dried Sliced Beef (Kilishi) Sold within Birnin Kebbi Metropolis, Kebbi State, Northern Nigeria. South Asian Journal of Research in Microbiology.DOI:10.9734/sajrm/2020/v8i330197. NPC (2006). National Population Commission, Nigerian population census. Abuja, Nigeria. Okonko, I.O, Odu, N.N and Igbo, I.E. (2013). Microbiological analysis of Kilishi sold in Port Harcourt, Nigeria.New York Science Journal, 6 (7):37-43 Okwori, A.E.J., Ibioha, C., Olabode, A.O, Etukudoh, N.S., Lugos, M.D., Turay, A.A., Udeani, T.K.C., and Okwori, E. (2009). Bacteriology of Dried Meat (Kilishi) Hawked in Some Northern Nigerian Cities. Nigerian Journal of Biotechnology, 20: 1-6. Raji, A.I. (2006). Bacteriological Quality of Dried Sliced Beef (Kilishi) Sold In Ilorin Metropolis. Journal of Applied Science Environmental Management 10 (1): 93 – 96. Salihu, M.D, Junaidu, A.U., Magaji, A.A., Aliyu, R.M., Yakubu, Y., Shittu, A., and Ibrahim, M.A. (2010). Bacteriological quality of traditional prepared fried ground beef (Dan bun Nama) in Sokoto. Nigerian advance Journal of Science and Technology 2(3):145- 147 Shamsuddeen, U. (2009). Microbiological quality of spice used in the production of Kilishi, a traditionally dried and grilled meat product. Bayero Journal of Pure and Applied Sciences, 2(2): 66-69. Table 1. Result of Microbial Count of Kilishi samples in Owerri Metropolis Study Area Dilution factor TAC Pure culture TCC Pure Culture EMB Pure Culture Indole Citrate Utilization Catalase TSA Isolate Dougl as a b 10-7 4.1×10-8 3.1×10-8 1.8×108 2.2×10-8 _ _ + + Salmonella Obinze a b 10-7 3.0×10-8 3.4×10-8 3.0×10-8 2.4×10-8 + + - + - E. coli World bank a b 10-7 4.3×10-8 3.3×10-8 2.0×10-8 1.6×10-8 + + ̶ - ̶ + + ̶ + E. coli Salmonella New Owerri a b 10-7 4.9×10-8 5.5×10-8 1.1×10-8 1.5×10-8 ̶ + + + Salmonella Ekene bu a b 10-7 5.4×10-8 4.6×10-8 2.0×10-8 1.6×10-8 + - - - + + + + E. coli Salmonella Table 2. Result of the Biochemical test of Kilishi samples obtained from different vendors in Owerri Metropolis Sample sites Dilution factor TCC Pure culture EMB Pure Culture DCA Pure Culture Indole Citrate Utilization Catalase TSA Isolates Douglas 10-7 + ̶ + + + Salmonella Obinze 10-7 + + + ̶ ̶ + + + ̶ + E. coli Salmonella World bank 10-7 + + ̶ + ̶ E. coli New Owerri 10-7 + + ̶ + + -__ + + + - Salmonella E. coli Ekenebu 10-7 + + + _ ̶ + + + ̶ _ E. coli Salmonella TCC= EMB=Eosin Methylene blue DCA= citrate Agar TSA= Tripthous Soy Agar Table 3: Occurrence of Bacterial contamination of Kilishi samples from Owerri metropolis (n=50) Location No. Samples Escherichia coli Salmonella spp. Number (%) Douglas 10 - + 1(10%) Obinze 10 + + 2(20%) World Bank 10 + _ 1(10%) New Owerri 10 + + 2(20%) Ekenebu 10 + + 2(20%) Total: 50 8/50 (16%)
