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Ilea Contractility Potential of Hydro-Ethanolic Extract of Oyster Mushroom ( Pleurotus Ostreatus ) on Guinea Pig ( Cavia Porcellus ) Ileum

Zitte, Leelee Famii Konya Roseline Sornayee

Abstract

Ileum of animals demonstrate responses to contractile potentials of acetylcholine and its agonist herbal extracts in an isolated tissue experiment. Contractile potentials of hydro-methanolic extract of oyster mushroom (Pleurotus ostreatus) on isolated ileum of guinea pig (Cavia Porcellus) was studied. Ten milligram (10mg) of acetylcholine was serially diluted to obtain a concentration of 0.1ug/ml, 1ug/ml, 10ug/ml, 100ug/ml and 1mg/ml. P. ostreatus hydro- methanolic extract was also diluted from a stock solution of 200mg/ml to obtain a serial dilution of 20ug/ml, 200ug/ml, 2mg/ml, and 20mg/ml. Tyrode solution was used as Physiological solution for the study. Guinea pig ileum cut at 10cm from the ileocecal junction was use for the contractility study. Different concentrations of acetylcholine were used as single administrations and in combination with Pleurotus ostreatus. This was used to determine the effects of Pleurotus ostreatus hydro-ethanolic extracts on the enteric acetylcholine receptors, the results showed that the P. ostreatus extract demonstrates a physiological agonist potential to acetylcholine. It expressed contraction inducement similar to acetylcholine-induced contraction on the invitro ileum contractility test without necessarily occupying the same receptor.

Keywords

Pleurotus ostreatusguinea pig ileumacetylcholineatropine

References

Blumenthal DK.(2018) Pharmacodynamics: Molecular mechanisms of drug action. In: Goodman and Gilman’s Pharmacological Basis of Therapeutics, Edn 13, McGraw-Hill Education, New York :31-54 Cantoni, G.L. and Eastman G. (1946) “On the response of the intestine to smooth muscle stimulants”.Journal of Pharmacology and Experimental Therapeutics 87:392–399. Chen KB, Wu ZW, Wang J, Zhu LH, Jin XL, Chen GF, Kang MX, Huang Y, Zhang H, Lin LL, Shi DK, Wu D, Chen JF, Chen J, Zhao ZQ.Trials. 2022 Mar 3;23(1):189. doi: 10.1186/s13063-022-06108-z.PMID: 35241130 Dale, M.M (1958) An inhibitory effect of acetylcholine on the response of the guineapig ileum to histamine. British Journal of Pharmacology 13:17–19. Eglen, R.M., Adham, N., and Whiting, R.L. (1992) “Acute desensitization of muscarinic receptors in the isolated guinea-pig ileal longitudinal muscle”. Journal of Autonomic Pharmacology 12:137–148. Fabisiak A, Wlodarczyk J Fabisiak N Storr M, Fichna J (2017): Targeting histamine recptors and irritable bowel syndrome : A critical appraisal Journal of Neurogastroenterology and Motility,: 23(3): 341 – 8 Doi: 10.5056jnml 6203 Garcia-Lopez P, Garcia-Marin V, Martínez-Murillo R, Freire M. (2009)Updating old ideas and recent advances regarding the Interstitial Cells of Cajal. Brain Res Brain Res Rev 61: 154–169 Ge Z, Duan Z, Yang H, Zhang S, Zhang S, Wang L, Yang D, Sun X, Zhang Z, Su L, Zhu H, Zhou D, Liu B, Shi H, Yu J, Yang H, Chang Q, Zhang N, Wu D, Chen JDZ(2018). Evid Based Complement Alternat Med. Apr 29;2018:2086163. doi: 10.1155/2018/2086163. Collection 2018.PMID: 29853946 Himpens, B, Droogmans G, and Casteels R (1991) Carbachol-induced nonspecific desensitization in guinea-pig ileum. Naunyn-Schmiedeberg’s Archives of Pharmacology 343: 580–587. Konya RS (2002). Potent vasoconstriction of mesenteric vessels by the venom extract of the sea anemone , Tealia felime ,Nigeria Journal of physiological Science 17(1-2): 30-35. Murakami H, Li S, Foreman R, Yin J, Hirai T, Chen JDZ.J Neurogastroenterol Motil. 2019 Apr 30;25(2):286-299. doi: 10.5056/jnm18094.PMID: 30827069 Free PMC article. Paton W.D.M. (1961) “A theory of drug action based on the rate of drug-receptor Combination”. Proceeding of the Royal Society of London Series B 154:21–69) Sanders KM, Koh SD, Ward SM. (2006) Interstitial cells of cajal as pacemakers in the gastrointestinal tract. Annu Rev Physiol 68: 307–343. Thomas EA, Baker SA, and Ehlert FJ (1993) Functional role for the M2 muscarinic receptor in smooth muscle of guinea pig ileum. Mol Pharmacol 44:102–110

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