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Evaluation of Watermelon Seed ( Citrullus Lanatus ) Formulated as An Alternative Culture Media for Selected Test Bacteria

Arikekpar Ibemologi and Emmanuel, Kome Gift

Abstract

Microorganisms rely on essential nutrients, a suitable energy source, and optimal environmental conditions to thrive. Traditionally, their growth has been facilitated using culture media. These media are designed to provide macro-elements which are vital for synthesizing biological macromolecules, but their high cost has driven the search for affordable alternatives. This research was designed to evaluate the potential of harnessing watermelon seeds as an alternative culture media for the growth of some selected test bacteria. The proximate analysis of watermelon seeds (Citrullus lanatus) consists of moisture content (5.6%), ash content (2.1%), crude lipids or fat (51.5%), crude fiber (3.1%), crude protein (28.9%), carbohydrate (8.8%), and calorific content (561.887Kcal). Watermelon agar was formulated by dissolving 15g of agar, 0.5g of sodium chloride, 0.75g of grinded watermelon seeds into 100ml of distilled water, at setting temperature of 36.8°C and at pH of 6.78. The total Colony Count (CFU/mL) and Population density of test bacteria cultured on the formulated agar were as follows: Escherichia coli (2.1 x 1010 CFU/mL), Staphylococcus aureus (9.4 x 109 CFU/mL), Klebsiella pneumoniae (5.4 x 108 CFU/mL), Proteus vulgaris (1.8 x 109 CFU/mL), Pseudomonas aeruginosa (1.8 x 109 CFU/mL). Water melon agar can be easily formulated and can also be deployed for isolation of bacteria in low income countries.

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