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The Effect of Imidacloprid on Organosomatic Indices and Haematological Parameters of Clarias Gariepinus

Puanoni, Azibahone Ragan, Prof. Iniobong Reuben Inyang, Lucky, Fineroad

Abstract

The vulnerability of the aquatic ecosystem to neonicotinoid pesticides has resulted to toxicity of the aquatic organisms. Sixty adult Clarias gariepinus (mean weight 318±10 g; mean length 32±2 cm) obtained at Department of fisheries farm in Niger Delta University were exposed to sub-lethal concentrations of imidacloprid (0.8, 1.6, 2.4, and 3.2 mg/L) for 21 days under controlled laboratory conditions. The study aimed to assess the sub-lethal effects of this neonicotinoid pesticide on haematological parameters and organosomatic indices in this ecologically and commercially important freshwater species. Hematological results revealed concentration-dependent changes. RBC counts initially rose from 1.8 × 106/?L (0.1 mg/L) to 2.0 × 106/?L (1.6 mg/L), then dropped to 1.5 × 106/?L (3.2 mg/L) compared to 1.3 × 106/?L in controls, indicating early erythroid stimulation followed by hemolysis. Hemoglobin increased to 9.4 g/dL (0.8 mg/L) but fell to 6.9 g/dL (0.4 mg/L), suggesting hypoxia. Hematocrit peaked at 40% (1.6 mg/L) before declining, reflecting compensatory erythropoiesis and subsequent anemia. WBC counts spiked to 57.1 × 103/?L (0.8 mg/L) compared to 17.6 × 103/?L in controls, then dropped to 28.1 × 103/?L (3.2 mg/L), indicating physiological?stress. Organosomatic indices revealed organ-specific damage, with liver, spleen, heart, and kidney somatic indices decreasing by 23%, 41%, 30%, and 26.4% respectively at 3.2 mg/L compared to the control. These changes point to systemic and physiological impairment. This findings has confirm that even sublethal imidacloprid exposure can induce marked hematological disruptions and organ dysfunction. There is need for urgent need to adopt more sustainable pest management strategies.

References

Abdel Rahman, H., Mohamed, A., & Ahmed, S. (2022). Effects of imidacloprid exposure on growth and immune response of Clarias gariepinus. Aquaculture Reports, 23, 101012. https://doi.org/10.1016/j.aqrep.2022.101012 Adewole, S. O. (2020). Organosomatic indices as biomarkers in fish exposed to environmental contaminants. Journal of Environmental Biology, 41(6), 1243–1251. https://doi.org/10.22438/jeb/41/6/1243-1251 Américo-Pinheiro, J., de Souza, R., & Faria, M. (2019). Hematological and immunological responses of fish under pesticide stress. Environmental Science and Pollution Research, 26(35), 35674–35685. https://doi.org/10.1007/s11356-019-06600- Ansoar-Rodríguez, I., López-Fierro, P., & Ramírez, R. (2016). Imidacloprid-induced oxidative stress and genotoxicity in Nile tilapia (Oreochromis niloticus). Chemosphere, 144, 1317–1324. https://doi.org/10.1016/j.chemosphere.2015.10.017 Beketov, M. A., Kefford, B. J., Schäfer, R. B., & Liess, M. (2013). Pesticides reduce regional biodiversity of stream invertebrates. Proceedings of the National Academy of Sciences, 110(27), 11039–11043. https://doi.org/10.1073/pnas.1305618110 Britz, P. J., Hecht, T., & Davies, B. R. (2017). Clarias gariepinus as a bioindicator species for freshwater ecotoxicology. Aquaculture International, 25(1), 187–203. https://doi.org/10.1007/s10499-016-0071-2 Crosby, D. G., Roberts, J. L., & Bouchard, P. (2015). Neurotoxic and physiological effects of neonicotinoids in non-target aquatic species. Environmental Toxicology and Chemistry, 34(12), 2825–2834. https://doi.org/10.1002/etc.3153 Cui, M., Li, X., & Zhang, Y. (2020). Combined toxicity of pesticides and heavy metals on freshwater fish. Science of the Total Environment, 714, 136715. https://doi.org/10.1016/j.scitotenv.2020.136715 Fazio, F. (2019). Hematological biomarkers in fish toxicology. Comparative Biochemistry and Physiology Part C, 224, 1–12. https://doi.org/10.1016/j.cbpc.2019.03.001 Gilliom, R. J., Barbash, J. E., Crawford, C. G., & Hamilton, P. A. (2006). Pesticides in the nation’s streams and groundwater, 1992–2001. U.S. Geological Survey Circular 1291. https://pubs.usgs.gov/circ/2005/1291/ Goulson, D. (2013). An overview of the environmental risks posed by neonicotinoid insecticides. Journal of Applied Ecology, 50(4), 977–987. https://doi.org/10.1111/1365-2664.12111 Jeschke, P., Nauen, R., Schindler, M., & Elbert, A. (2011). Overview of the status and global strategy for neonicotinoids. Pest Management Science, 67(11), 105–112. https://doi.org/10.1002/ps.2135 Mohamed, M., Ibrahim, K., & Osman, H. (2021). Organosomatic indices as indicators of fish health under chemical stress. Environmental Monitoring and Assessment, 193(6), 356. https://doi.org/10.1007/s10661-021-09045-8 Omitoyin, B. O., Ayoola, S. O., & Akinrotimi, O. A. (2006). Acute toxicity of imidacloprid to Nile tilapia (Oreochromis niloticus). Nigerian Journal of Fisheries, 3(1), 45–52. Pisa, L. W., Amaral-Rogers, V., Belzunces, L. P., Bonmatin, J.-M., Downs, C. A., Goulson, D., Kreutzweiser, D. P., Krupke, C., Liess, M., McField, M., & van der Sluijs, J. P. (2017). Effects of neonicotinoids and fipronil on non-target invertebrates. Environmental Science and Pollution Research, 24(1), 68–102. https://doi.org/10.1007/s11356-016-8103-4 Simon-Delso, N., Amaral-Rogers, V., Belzunces, L. P., Bonmatin, J. M., Chagnon, M., Downs, C., Furlan, L., Gibbons, D., Giorio, C., Girolami, V., Goulson, D., Kreutzweiser, D., Krupke, C., Liess, M., Long, E., McField, M., Mineau, P., Mitchell, E., Morrissey, C., ... Wiemers, M. (2015). Systemic insecticides (neonicotinoids and fipronil): Trends, uses, mode of action and metabolites. Environmental Science and Pollution Research, 22(1), 5–34. https://doi.org/10.1007/s11356-014-3470-y Sánchez-Bayo, F., Goka, K., & Hayasaka, D. (2016). Contamination of global surface waters with neonicotinoids and its implications for ecosystems. Frontiers in Environmental Science, 4, 71. https://doi.org/10.3389/fenvs.2016.00071 Tomizawa, M., & Casida, J. E. (2005). Neonicotinoid insecticide toxicology: Mechanisms of selective action. Annual Review of Pharmacology and Toxicology, 45, 247–268. https://doi.org/10.1146/annurev.pharmtox.45.120403.095930 Topal, A., Sener, E., & Yilmaz, N. (2017). Oxidative stress and immune response in fish exposed to neonicotinoids. Ecotoxicology, 26(2), 239–248. https://doi.org/10.1007/s10646-017-1805-x Tudi, M., Liu, H., Mainali, B., Roel, A., & Zhang, Z. (2021). Agriculture and aquatic pollution: Neonicotinoid pesticide impacts in Africa. Science of the Total Environment, 754, 142379. https://doi.org/10.1016/j.scitotenv.2020.142379 Ullah, I., Khan, S., & Khan, M. (2018). Sublethal effects of imidacloprid on freshwater fish. Environmental Toxicology and Pharmacology, 61, 69–77. https://doi.org/10.1016/j.etap.2018.09.001 Van der Oost, R., Beyer, J., & Vermeulen, N. P. E. (2003). Fish bioaccumulation and biomarkers in environmental risk assessment: A review. Environmental Toxicology and Pharmacology, 13(2), 57–149. https://doi.org/10.1016/S1382-6689(02)00126-6

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