Hypoglycaemic Effect of Black Lipton Extract ( Camellia sinensis ) on AlloxanInduced Diabetic Wistar Rat

Zitte L.F.; Peter; P.O.

doi:10.56201/jbgr.vol.11.no6.2025.pg47.52

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Hypoglycaemic Effect of Black Lipton Extract ( Camellia sinensis ) on AlloxanInduced Diabetic Wistar Rat

Zitte L.F. and Peter, P.O.

Abstract

This Study was conducted to investigate the hypoglycaemic effects of Black Lipton extract on alloxan-induced diabetic Wistar rats. Twenty (20) Wistar rats weighing between 55 -100g were obtained from the Animal House of the Faculty of Pharmaceutical Science, University of Port Harcourt, Nigeria. The rats were divided into five groups of four animals in each groups. Group 1 was the non-diabetic control group. Groups 2, 3, 4 and 5 were induced with diabetes by administering intraperitoneal injection of alloxan, 180mg/kg. Development of diabetes was observed in 3 days (72 hours) after which the blood glucose levels were measured and determined by the method of glucose kit analysis. Following diabetes confirmation through blood glucose measurement, Group 2 was used as the diabetic control group, while groups 3, 4 and 5 received 200mg/kg, 400mg/kg and 800mg/kg of the extract respectively. Blood glucose levels were checked every 3 days at regular intervals over a 12 days treatment period. Results indicated a significant reduction in blood glucose levels in the treated groups (group 3, 4 and 5) from 7.48±0.07 to 4.95±0.06 (group 3), 7.45±0.08 to 4.7 ±0.09 (group 4), 7.58±0.16 to 4.42±0,19 (group 5). While the diabetic control (group 2) had it glucose level at 7.3±0.09 after inducement and 9.05±0.14 after 12 days. This research reveals that Black Lipton extract may possess antidiabetic properties. The findings highlight the potential of Black Lipton as a natural therapeutic agent for managing diabetes.

Keywords

HypoglycaemicBlack Lipton extractAlloxan-induced diabetesAntidiabetic

References

Ahmad, N., Feyes, D. K., Agarwal, R., Mukhtar, H., & Nieminen, A. L. (1997). Green tea constituent epigallocatechin-3-gallate and induction of apoptosis and cell cycle arrest in human carcinoma cells. Journal of the National Cancer Institute, 89(24), 1881-1886. Aizaki, T., Osaka, M., Hideyuki, H. A., Kurokawa, S., Matsuyama, K., & Izumi, T. (1999). Hypokalemia with syncope caused by habitual drinking of oolong tea. Internal Medicine, 38(3), 252-256. Baynes, J. W., & Thorpe, S. R. (1999). Role of oxidative stress in diabetic complications: A new perspective on an old paradigm. Diabetes, 48(1), 1-9. Broadhurst, C. L., Polansky, M. M., & Anderson, R. A. (2000). Insulin-like biological activity of culinary and medicinal plant aqueous extracts in vitro. Journal of Agricultural and Food Chemistry, 48(3), 849-852. Dulloo, A. G., Seydoux, J., Girardier, L., Chantre, P., & Vandermander, J. (2000). Green tea and thermogenesis: Interactions between catechin-polyphenols, caffeine, and sympathetic activity. International Journal of Obesity, 24(2), 252-258. Hasanein, M. A., Gawad, H. S., & El-Megeid, A. A. (2012). Effect of water extract prepared from green tea, black tea, and cinnamon on obese rats suffering from diabetes. World Applied Sciences Journal, 20(7), 976-987. King, H., Aubert, R. E., & Herman, W. H. (1998). Global burden of diabetes, 1995-2025: Prevalence, numerical estimates, and projections. Diabetes Care, 21(9), 1414-1431. Li, S., Lo, C. Y., Pan, M. H., Lai, C. S., & Ho, C. T. (2013). Black tea: Chemical analysis and stability. Food & Function, 4(1), 10-18. Nelson, D. L., Lehninger, A. L., & Cox, M. M. (2008). Lehninger principles of biochemistry. Macmillan.

Hypoglycaemic Effect of Black Lipton Extract ( Camellia sinensis ) on AlloxanInduced Diabetic Wistar Rat

Abstract

Keywords

References

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