Submit your papersSubmit Now
For Enquiries: [email protected]
IIARD LogoIIARD

Antibacterial Activity and Phytochemical Analyses of Lawsonia Inermis (Henna) against Bacterial Isolates from Whitlow Patients Attending Selected Hospitals within Gusau Metropolis, Zamfara State

Kabiru Isah, Ahmad Ibrahim Bagudo, Garba Gidandawa Jibo, Adamu AlMustapha, Aliero, Sadiya Khaleed Adamu

Abstract

The aim of this research is to assess the antibacterial activity and phytochemical composition of Lawsonia inermis leaves extracts against bacterial isolates from whitlow patients. This study involved total of 70 swabs collected using swab stick from infected whitlow attending Federal Medical center Gusau Farida General Hospital Gusau, Ahmad Sani Yariman Bakura specialist Hospital Gusau all in Gusau metropolis which was processed for isolation and identification of bacterial isolates according to the standard microbiological techniques. Phytochemical screening of the extract was conducted using conventional method. Antibacterial activity of the extract was conducted by means of agar well diffusion method while broth dilution method was used for determination of minimum inhibitory concentration of the extract. The phytochemical screening of aqueous, ethyl acetate and methanol extracts of the plant indicated the presence of flavonoids, alkaloid, phenol compound, resins, tannins, steroids and saponins, glycosides, and terpenoid. From the results, methanol extract demonstrated higher activity producing an average activity of 16.58 mm, followed by aqueous extract with average zone of inhibition of 16.33 mm while ethyl acetate has an average of 15.16 mm. It is concluded that the extracts of L. inermis contain bioactive components that are active against bacteria associated with whitlow

Keywords

Antibacterial activityGusauLawsonia inermisphytochemicals

References

Akharaiyi, F. C. (2011) Antibacterial, Phytochemical and Antioxidant Activities of DaturaMetelInternational Journal of Pharmaceutical Technology Research. 3(1): 478- Ali, M., Aminu, F. & Ibrahim, I. S. (2017). In-vitro Assessment of Antibacterial Activity and Phytochemical Screening of Vitex doniana on Clinical Isolate of Salmonella typhi, International Journal of Adanced Academic Research, Science, Technology and Engineering 3(1): PP 9-16 Al-Rubiay, K.K., Jaber, N.N., Al-Mhaawe, B.H., `Alrubaiy, L.K. (2008). “Antimicrobial efficacy of henna extracts”. Oman Med. J. 2008;23(4):1-4. Amit and Singh, N. (2022). Studies on antimicrobial activity of Lawsonia inermis L. against different strains of bacteria and fungi. Journal of Applied and Natural Science, 14(1), 247 - 253. https://doi.org/10.31018/jans.v14i1.3337 Batiha, G. E. S., Teibo, J. O., Shaheen, H. M., Babalola, B. A., Teibo, T. K. A., Al-Kuraishy, H. M., ... & Papadakis, M. (2023). Therapeutic potential of Lawsonia inermis Linn: a comprehensive overview. Naunyn-Schmiedeberg's Archives of Pharmacology, 1-16. Chaachouay, N., Douira, A., and Zidane, L. (2021). Herbal Medicine Used in the Treatment of Human Diseases in Northern Morocco. Arabian Journal for Science and Engineering. 47(1): 131-153. De-boer, J.H., Kool, A., Broberg, A. and William, R.M. (2005). Anti-fungal and antibacterial activity of some herbal remedies from Tanzania. J. Ethnopharmacol. 96(3):461-469. Elaguel, A., Kallel, I., Gargouri, B., Amor, I. B., Hadrich, B., Mesaaoud, E. B., et al. (2019). Lawsonia inermis essential oil: Extraction optimisation by RSM, antioxidant activity, lipid peroxidation, and antiproliferative effects. Lipids in Health and Disease, 18(1), 196. El Massoudi, S., Zinedine, A., Rocha, J. M., Benidir, M., Najjari, I., El Ghadraoui, L., Benjelloun, M., & Errachidi, F. (2023). Phenolic composition and wound healing potential assessment of Moroccan henna (Lawsonia inermis) aqueous extracts. Cosmetics, 10, 92 Garba, M.U., Adamu, F.U., Mairami, F.M., Basheer, A., Aminu, M.A., Bukar, F.M. Yakubu, U.I. and Nura, S. (2025). Assessment of Antibacterial Potentials of Lawsonia inermis L. (Henna) Leaf Aqueous Extracts UJMR, Conference Special Issue Vol. 10 No. 3. pp. 389 - 394 https://doi.org/10.47430/ujmr.25103.039 Güler, ?., Torul, D., Kurt-Bayrakdar, S., Tayyarcan, E. K., Çamsar?, Ç., & Boyac?, ?. H. (2023). Evaluation of antibacterial efficacy of Lawsonia inermis Linn (Henna) on periodontal pathogens using agar well diffusion and broth microdilution methods: An in-vitro study. BioMedicine, 13(3), Article 3. Idris, A.H., Z. Haruna, M. Y. Iliyasu, M. R. Sahal, T. Inusa, A. Salisu, S. Isma’il, R. D. Umar, Z. M. Kabeer, H. Tahir, H. S. Musa and A. F. Umar (2023) Antibacterial Activity of Lawsonia inermis Leaf Extracts against Multidrug-resistant Pseudomonas aeruginosa from Infected Wounds European Journal of Medicinal Plants Volume 34, Issue 4, Page 1-8, DOI: 10.9734/EJMP/2023/v34i41130 Jothipraksam, V., Ramesh, S. and Kumarn, R.S. (2013). Preliminary phytochemical screening and antibacterial activity of Lawsonia inermis (henna) leaf extracts against reference bacterial strains and clinically important AMPC beta-lactamases producing Proteus mirabilis. Intl. J. Pharm. Pharm. Sci. 5(1):219–222. Matsuura, G.T and Barg, N. (2013). Antimicrobial management of foot infections in patients with diabetes. J. Clin. Diabetes. 2013;31: 59-65. Merdaw, M.A. (2009). Inhibition of bacterial growth by Lawsonia inermis (henna) leaf extracts in vitro. Ibn Al-Haitham J. Pure and Appl. Sci. 22(4):32. Meutia, N., Putra, B., & Jusuf, N. K. (2021). Antifungal activity of henna leaf extract (Lawsonia inermis Linn) against inhibition of Trichophyton rubrum fungal growth cause Tinea unguium. Jurnal Buletin Farmatera, 6, 72–79. Nas, F.S. & Ali, M. (2017). Antibacterial Activity of Boswellia dalzielii Leaves Extracts against Some Pathogenic Bacterial Isolates Journal of Advances in Microbiology 7(1): 1-8, DOI: 10.9734/JAMB/2017/37690 National Population Commission (NPC) (2022). Nigeria Demographic & Health Survey,Abuja, Nigeria: National Population Commission & ICF Macro Shukla, S., Yogesh, G. K., Srivastava, S. K., & Srivastava, S. K. (2024). Charge transfer mediated coercivity enhancement in Cu0. 26Ni0. 74 alloy nanoparticles on blending them with carbon nanotubes. Applied Physics A, 130(1), 31. Singh, D.K., Cheema, H.S., Saxena, A., Singh, S., Darokar, M.P., Bawankule, D.U., & Luqman, S. (2017). Fraxetin and ethyl acetate extract from Lawsonia inermis L. ameliorate oxidative stress in P. berghei infected mice by augmenting antioxidant defence system. Phytomedicine, 36, 262-272. Tiwari, P., Kumar, B., Kaur, M., Kaur, G. & Kaur, H. (2011). Phytochemical screening & extraction: a review. Internationale Pharmaceutica Sciencia. 1(1):98-106. Yahaya, A., Ali, M., EL- Hassan, F. I. & Jido, B. A. (2019). Antibacterial Activity of Guava (Psidium Guajava L.) Extracts on Staphylococcus Aureus Isolated from Patients with Urinary Tract Infections Attending a Tertiary-Care Hospital. Science World Journal 1 (1):47 – 51 Youl, O., Moné-Bassavé, B. R. H., Yougbaré, S., Yaro, B., Traoré, T. K., Boly, R., et al. (2023). Phytochemical screening, polyphenol and flavonoid contents, and antioxidant and antimicrobial activities of Opilia amentacea Roxb. (Opiliaceae) extracts. Applied Biosciences, 2, 493–512

More Articles from RESEARCH JOURNAL OF PURE SCIENCE AND TECHNOLOGY

Advances In NLP-Driven Analytics for Automated Detection of Regulatory Liabilities in High-Volume Contracts

Author: Ngonadi Uchechi, Michael Ominyi, Ngozi Samuel Uzougbo, Blessing Chika Jones,, USA

Resilient Test Automation Framework for Microservices: Addressing Integration, Scalability, and Reliability

Author: Awonowo Olusegun Oriyomi,, Anrinnle Qowiu Olaoluwa, Lawal Ahmed Oladimeji, Babayemi Temitayo Matthew, Azubuike Marvelous Onyedikachukwu, Olomu Ayomide Babatunde, Omitogun Ayomide Elijah