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Status of Selected Oxidative Stress and Cardiac Biomarkers in Rattus norvegicus (Norway Rats) Exposed to Mosquito Coil Smoke

Egoro, E.T.; Oni, E.S., Ezeiruaku F. C., George G.S.

Abstract

Mosquitoes are vectors that are responsible for malaria, thus many families have indulged in using mosquito coil in order to control its spread. This study aimed to investigate the status of selected oxidative stress and cardiac biomarkers in Rattus norvegicus exposed to mosquito coil smoke. Two milliliters of blood specimens were withdrawn via cardiac puncture technique from five groups of five rats per group with mean weight 0.410 ± 0.05 kg exposed to approximately 15 mg concentration of mosquito coil smoke / day for 30, 60, 90 and 120 days (experimental groups) respectively with the exception of the control group while 32 additional rats were used for pilot study and LC50 determination. This was followed by the measurement of oxidative stress biomarkers such as glutathione peroxidase (ultra violet) method, malondialdehyde (thiobarbituric) method, 8-hydroxy-2-deoxyguanosine (enzyme linked immunosorbent) method, and cardiac biomarkers such as troponin-1 (immune turbidimetric) method and creatinekinase-MB (immune inhibition) method. The data were analyzed using SPSS version 23.0 (SPSS Inc., Chicago, IL, USA; version 23.0). The differences in the various parameters studied among the tests and control groups were evaluated using Kolmogorov-Simirnov. Student t-test and analysis of variance (ANOVA) were used for comparing values of the measured biochemical parameters between the control and experimental groups with all post hoc testing done using Turkey HSD and Games-Howell methods where applicable. Data were considered significant at p < 0.05. The results revealed significant mean value elevation of glutathione peroxidase and malondialdehyde (p < 0.05) in the experimental rats exposed for 60, 90 and 120 days respectively as compared to control rats with no significant alteration (p > 0.05) revealed in the 30 days exposed experimental rats as compared to that of the control rats. Besides, the mean values of 8-Hydroxy-2-Deoxyguan

Keywords

Mosquito coil; Status; Oxidative stress biomarkers; cardiac biomarkers; Rattus norvegicus

References

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