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Determination of Voltage and Current Generation of Pseudomonas Aeruginosa Isolated from Wound, Urine, and Water Samples

Imrana Adamu Musa; Mohammed Ali Yobi

Abstract

This study investigates the electricity generation capacity of Pseudomonas aeruginosa in microbial fuel cells (MFCs), with a specific focus on how the bacterium's source environment influences its performance. The research is grounded in the theoretical framework of MFCs as bio-electrochemical systems, where electrogenic bacteria oxidize organic matter and transfer electrons to an anode. A key premise is that bacterial metabolic adaptations to their native habitats—such as nutrient-rich clinical sites versus nutrient-limited environmental sources— directly impact their electron transfer mechanisms. The study addresses a recognized gap in the literature: the lack of direct, comparative analysis of the electrogenic potential of P. aeruginosa isolates from diverse clinical and environmental origins under identical conditions. To achieve this, the work isolates and identifies P. aeruginosa from wound, urine, and water samples. The methodology involves cultivating each isolate in a dual-chamber MFC and measuring the voltage and current output daily over seven days. Results demonstrate that all isolates successfully generate bioelectricity, with performance peaking on the sixth day. The isolate from urine produces the highest output, followed by the wound and water isolates, respectively. This study concludes that the source of isolation significantly affects the electrogenic capacity of Pseudomonas aeruginosa, underscoring the importance of strain selection based on ecological origin for optimizing MFC applications.

Keywords

Electrogenic microorganismBacteriaMicrobial Fuel Cells (MFCs)Pseudomonas

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