Toxicological Evaluation of Some Physiological Parameters of Dietary Bambara nuts (Vigna subterrinea) In Clarias gariepienus
Abstract
Dietary Bambara nuts (Vigna subterrinea) was evaluated for its toxicological effects on sub- adult Clarias gariepinus. A 35%cp feed was formulated using local ingredients, and five different diets were produced by substituting soybean with various percentages of V. subterrinea as follows: T1(100%SB:0%VS), T2 (25%SB:75%VS), T3 (50%SB:50%:VS), T4 (75%SB: 25%VS) and T5 (0100SB:100%VS). One hundred and fifty (150) sub-adult C. gariepinus were distributed into five (5) groups and labelled T1 – T5, and were fed diets T1 – T5 respectively. Length and weights were taken, and feeding commenced twelve hours after stocking. After eight weeks feeding period, blood samples were taken from the fish in the various group to the laboratory for haematological parameters and antioxidant activities, and the fish were re-weighed to determine the condition factor of the fish. The results revealed that the fish fed V. subterriea had better antioxidant activities compared to the control (T1): SOD and CAT were higher in the fish fed T2 – T5 compared to T1, while MDA, GSH and GST were similar across T1 – T5. There were no significant difference in the PCV, HB, RBC, WBC and PLT in the fish fed T1 – T5, but the fish fed T2 – T5 were slightly higher in these parameters. The fish fed T2 – T5 had higher Lymphocytes (LYM) and lower neutrophil (NEUT) compared to T1, and fish fed T4 had a better condition factor compare to the rest. The replacement of soybean with V. subterrinea enhances conducive environment and growth in the culture of C. gariepinus.
Keywords
Clarias gariepinusVignia subterrineaHaematologyAntioxidant ActivitiesCondition Factor
References
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