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Physiological Response and Bacteriology of Dietary Ipomoea batatas Leaves Extracts on Clarias gariepienus Exposed to Pseudomonas aeruginosa

Ukwe, I.O.K and Nweke, I.P.

Abstract

Ipomoea batatas aqueous leaves extracts were examined to ascertain its phyto-prophylactic effect on Clarias gariepinus exposed to P. aeruginosa. Ipomoea batatas aqueous extracts was prepared by soaking 500g per liter of water for 12 hours. Five different diets were prepared from a 35%cp feed using various quantity of the extract: 0ml/kg (Do); 50ml/kg (D1); 100ml/kg (D2) and 150ml/kg (D3). One hundred and fifty (150) C. gariepinus were distributed into five groups in triplicates and fed accordingly with; D0, D1, D2 and D3 I. batatas supplemented diets. Two groups were fed with D0, positive and negative controls (D0+ve and D0-ve). At the end of eight weeks feeding period, the fish in groups D0-ve (negative control) and D1-D3 were infected with 1.5ml of 1.5 × 108 cfu/ml overnight grown Pseudomonas aeruginosa while the fish fed D0+ve was uninfected. After seven (7) days post infection period, blood samples were collected from all groups to determine the haematological parameters and the antioxidant activities [Pack cell volume (PCV), Hemoglobin (HB), Red blood cell (RBC), White blood cell (WBC), and Platelet (PLT), Superoxide dismutase (SOD), Malondialdehyde (MDA), Catalase (CAT), Glutathione (GSH) and Glutathione, -S-Tranferase (GST)]. The fish organs were also harvested for bacteriological analysis. The values of the RBC, PCV and Hb were significantly lower in the D-ve group compared to the fish in groups. D+ve, D1-D3. There was no significant difference in the values of EOS, MCH, and MCHC (p<0.05) across the groups. There were reduced activities in SOD and CAT in the plasma of the fish in D-ve compared to the other groups. The result of the bacteriological analysis reveals high presence of P. aeruginosa in the liver and kidney of the fish in D1-D3 compared to these organs in the fish in D-ve. Sequel to these results, it is evidential that I. batatas possess the potential to maintain normal haematological parameters, reduce str

Keywords

Clarias gariepinusphyto-prophylaticIpomoea batatasPseudomonas aeruginosadiseaseshaematological parametersantioxidantsactivities.

References

Amachree, D. and Ukwe, I. O. K. (2022).Effects of Perse aamericana supplemented diets on plasma Antioxidant levels of Clarias gariepinus and its prophylactic Effect after Klebsiella pneumonae infection IAR Journal of Agricultural Science and Food Research, 2(2): 8-13. Amisah, S., OTeng, M. & Oton, J. (2009). Growth Performance of the African Catfish. Clarias gariepinus fed varying inclusion levels of Leucoena leucocephala leaf meal. Journal of applied Sciences and Environmental Management, 13:21-26. Ardo, L., Yin, G., Xu, P., Varadi, L. Gzigeti, G. and Jeney, Z. (2008). Chinese herbs (Astragalus membraneous and lonicera japonica) and bofon enhance the nonspecific immune response of Nile titapia (Oreodiromus nitoticus) and resistance against Aerominas hydrophila Aquaculture, 275:26-33. Arguelles, S. Garcia, S.Maldonado, M. Machado and Ayala, A. (2007a). Do serum oxidative stress biomarkers provide a reasonable index of the general oxidative stress status? Biochimica et Biophysica Acta: General subjects, 1674(3) 251-259 Aziz M. A. Diab, A.S and Mohammed A.A (2019). Antioxidant categories and mode of action. Research Gate intechopen, 1-20. Bhattacharjere, A., Saha, N., Goswami, U., and Saikia, D. (2020). Probotic Streptococcus Pasteurianus HTR7R8 enhances the Immune response and disease resistance against Aeromonas hydrophila infection in India Major Carp, Labeo rohita. Aquaculture Reports, 4(2), 98-107. Bozzo, F., Moraes, F.R., Pereira, G.T., Tavares-Dias, M. and Onaka, E.M. (2007). Kihnetic, of cellular component in inflammatory response induced by different stimuli in the swim bladder of pacu piaractusmesopotamicus Holmberg, 1887 (Characide). Journal of world Aquaculture Society, 38(2), 302-308. Christybapita D Divyagnaneswari M and Micheal RD. (2007). Oral administration of Eccipta leaf aqueous extract enhance the non-specific immune responses and disease resistance of orechromis mossambicus. Fish shellfish immunology 23 (4); 840-852. Cipriano, C. Rocco.2001”Aeromonas hydrophila and Motile Aeromonad septicemias of fish” In fish disease, Washington united states Department of interior,65-97 Ehsan, R., Alam, M., Aktar, T., Paul, s. I., Foysal, M. J., Gupta, D. R., Islam, T. & Rahman, M. M. (2021). Enterococcus faecalis involved in streptococcosis like infection in silver barb (Barbonymus gonionotus). Aquaculture Reports. 21:100863. Farahi, A., Kasiri, M., Sudagar, M., Soleimani, M., Iraei, M., and Zorrielizahra, S. M. J. (2012). Effects of dietary supplementation of Melissa officinalis and Alovera on hematological traits, lipid oxidation of carcass and performance in rainbow trouts (Oncorhynchus mykiss). Online Journal of Animal feed Research, 1:1-5. Fawole, F.J., Sahu, N.P., Pal A.K and A. Ravindran, (2016). Haemato-immunological response of labbeo rohita (Hamilton) fingerlings fed leaf extracts and challenged by Aeromonas hydrophilla” Aqucaulture Research, Vol. 47 (12), pp. 3788 – 3799. Gabriel, N.N, Quiang J, Ma XY, He J, Xu P. and Liu K (2015). Dietary Aloe vera improve plasma liquid profile, antioxidant, and hepatoprotective enzyme activities in GIFTtilapia (Oreochromis niloticus) after Streptococcus iniae challenge. Fish physiology and Biochemistry. 4:1321 – 1332. Gidding, A. T. and Lambig, G. (2001). The immune system of fish: Kidney and Spleen. Immunology Volume (107), 3-10. GRFC (2022). Global report on food crises 6th edition. Food security information network. https://www.fao.org/3/eb9997,enfeb9997.pdf. Hamid, N.K.A., Somare, P.O., & Harashid, K.A.M. (2022). Effect of papaya (Carica papaya) leaf extract as dietary growth promoter supplement in red hybrid tilapia (orechromis mossambicus x orechromis niloticus) diet. Saudi Journal of Biological Science, 29 (5) 3911-3917, 2022 Hrubec, Terry C, Jennifer L. Cardonate and Stephen A Smith. (2000). Haematology and Plasma chemistry reference/intervals for cultured Tilapia (Oreochromis hybrid); Veterinary Clinical pathology 29(1): 1-12. Jana, Z., Thomas, S. H., Edmund, M., Stephan, G. Richardus Vonk & Reinhard L. (2010). Effects of synthectic gestations on fish production. Environmental Toxicology and Chemistry 28(12): 2663 – 2670. Krishnamurthy. P. and Wadhwani, A (2012). Antioxidant enzymes and human health. In EI missiry M.A., editor, Antioxidant enzyme. Croatia: In Tech, p.3-18. Liang Q.N, Sheng Y.C. Jang P,Jill, Xia Y. Y, Min Y. and Wang, Z.T. (2011). The gender- dependent difference of liver GSH antioxidant system in mice and its influence on Isoline induced liver injury. Toxicology 280 (1-2): 61-69. http:// doi.org/101016/j.tox.2010.11.010 Martins, M.L., Tavares. Dias, M; Fujimoto, R.Y., Onaka, F.M, and Nomura, D.T. (2004). Haematological alterations of leporimus macrocephalus (Osteichthye; Anostomidae) Naturally infected by Goezia Leporine (Nematoda: Anisakidae) infish pond. Arq. Bras. Med. Vet. Zootec., 56(5), 640-646. Nnabuchi, O., Ejikeme, O.G., Nwani, C.D., Ochamh, S., Somdare; P., and Amarachi, A.C. (2015). Effect of Parasites on the biochemical and haematologival indices of some clarivd (siluriformes) catfishes from Anambra River, Nigeria. International Journal of Fish Aquatic Studies; 3(2) 331-336. Obasohan, E. E., Agbonlahor, and E. E. Obano. 2010. “Water Pollution: A Review of Microbial Quality and Health Concerns of Water, Sediment and Fish in the Aquatic Ecosystem.” African Journal of Biotechnology 9 (4): 423–27. Obum-Nnadi C.N, Amaechi D., Ezenwa C.M., Esonu U., K.S. Nwokorie and Ajima Mary (2022). Anti-Bacterial Phytochemical Analysis and Blood pressure lowering effects of Orange flesh sweet potatoes (Ipomoea Batatas), African Journal of Biotechnology, 10(5) 20-40 Oghenochuko, O. N. and Mshelbwala, F. M. (2021). Effects of Moringa oleifera Allium Sativum, Zingiber Officinale on the haemotological parameters and histopathological changes in visceral organs of Clarias gariepinus infected with Pseudomonas aeruginosa. International Journal of Fish Aquatic Studies; 5(4) 302-326. Omran, F., Matar, N., and El-Masry, S. (2003). Some haematological and biochemical responses of Clarias lazera to Aeromonas hydrophila. Journal of Fish Diseases, 26(4), 345-351 Prochazkova. D, Bousova.I and Wilhelmova (2011). Antioxidant and prooxidant properties of flavonoids. Fitoterapia, 82:513-523. Putri, E.P.K., Hamzah. B. and Rahman. N (2013). Qualitative analysis of bioactive substance in avocado (Persea Americana) leaf extract and preclinical testing in lowering blood glucose level in mice (mus musculus). Journal Akad. Kim, 2:119-127, (In Indonesian) Rajendiran, R., Soosairaji, N. Ramamoorthy, S. and Werson, S. (2008). Immunoprophylaxis against Aeromonas hydrophila Infection in Freshwater Murrel, Channa Punctatus using different doses of Solanum nignim vaccine. Journal of Aquatic Animal Health, 20(4), 289- Saei M, Blykabi and Ellis, A.E. (2007). Effect of enteric bacteria on phagocyte and lymphocyte population in Rainbow trout on corhynchus mykiss (walbaum) journal. Fish and Shell fah Immunology (25) 803-811. Site, J. B., Johnson, A. and Ngadi, A. (2021). Aeromonas hydrophila infection in Clarias gariepinus in vitro and in vivo efficacy of Acanthus licitollis leaves extract, Journal of Phamategnosy and Phytochemistry, 10(2), 786-791. Talib, K. R., and Zakaria, M. R. (2018). The effect of sweet potato (Ipomoea batatas) leaves on immune response, growth performance, and disease resistance of common carp (Cyprinus carpio). Journal of Applied Aquaculture, 30(1), 50-63. Tewary, A. and Patra B.C. (2008). Use of vitamin C as an immunostimulant. Effect on growth, nutritional quality and immune response of labeo rohita (Ham). Fish physiology and Biochemistry. 34:251-259 Thomas, J., Thanigaivel, S., Vijayakumar, S., Acharya, D., Seelan, T.S., Mukherjee, A., and Chandrasekaran, N., (2014). Pathogenici