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Phytochemical Profiling, Biosafety Evaluation, and Antimicrobial Activity of Five Plant Extracts for Sustainable Applications

Ezechinyere-Otuka, U P, Stanley, H O, IbezimEzeani MU

Abstract

The growing need for sustainable and eco-friendly solutions in water treatment has increased interest in plant-based alternatives with proven bioactivity and safety. This study investigates the phytochemical composition, biosafety profile, and antimicrobial activity of five indigenous plant extracts, Cymbopogon citratus, Trichostema lanceolatum, Senna alata, Ficus exasperata, and Moringa oleifera, to evaluate their potential for sustainable applications. Proximate analysis revealed variations in protein, fibre, and nitrogen-free extract content, with Moringa oleifera recording the highest carbohydrate concentration and T. lanceolatum the highest protein content. Qualitative and quantitative phytochemical screening confirmed the presence of key secondary metabolites, including tannins, saponins, flavonoids, phenols, alkaloids, terpenoids, and cardiac glycosides, across all samples, with M. oleifera exhibiting the most abundant profile. Biosafety assessments indicated that all extracts were free from microbial contamination and within acceptable limits for heavy metals, although F. exasperata showed slightly elevated levels of iron and lead. Mild skin irritation was observed only with C. citratus. Antimicrobial tests showed variable effectiveness, with M. oleifera demonstrating the highest antibacterial and antifungal activities. These findings support the potential use of these plant extracts, particularly M. oleifera and S. alata, as effective, low-toxicity agents for antimicrobial and water treatment purposes. This study reinforces the role of phytochemicals in environmental remediation and provides a scientific foundation for integrating plant-based solutions into sustainable water purification strategies.

Keywords

Phytochemicals; Antimicrobial activity; Biosafety; Moringa oleifera; Sustainable

References

Adeeyo, A. O., Edokpayi, J. N., Alabi, M. A., Msagati, T. A., & Odiyo, J. O. (2021). Plant active products and emerging interventions in water potabilisation: Disinfection and multi-drug resistant pathogen treatment. Clinical Phytoscience, 7, 1–16. https://doi.org/10.1186/s40816-021-00279-3 Aliyu, L. D., Abdullahi, M., & Ibrahim, M. (2016). Effects of extraction methods on phytochemical yield in Moringa species. African Journal of Biotechnology, 15(20), 844– https://doi.org/10.5897/AJB2016.15336 Al-Jadabi, N., Laaouan, M., El Hajjaji, S., Mabrouki, J., Benbouzid, M., & Dhiba, D. (2023). The dual performance of Moringa oleifera seeds as eco-friendly natural coagulant and as an antimicrobial for wastewater treatment: A review. Sustainability, 15(5), 4280. https://doi.org/10.3390/su15054280 Baptista, A. T. A., Coldebella, P. F., Cardines, P. H. F., Gomes, R. G., Vieira, M. F., Bergamasco, R., & Vieira, A. M. S. (2015). Coagulation–flocculation process with ultrafiltered saline extract of Moringa oleifera for the treatment of surface water. Chemical Engineering Journal, 276, 166–173. https://doi.org/10.1016/j.cej.2015.04.066 Benalia, M., Bradai, M., Zerizer, S., & Mahdhi, A. (2024). Antibacterial and coagulant activity of selected plant extracts: Implications in water treatment. Water Supply, 24(2), 345–358. https://doi.org/10.2166/ws.2023.283 Bolade, O. P., Akinsiku, A. A., Adeyemi, A. O., Williams, A. B., & Benson, N. U. (2018). Dataset on phytochemical screening, FTIR and GC–MS characterisation of Azadirachta indica and Cymbopogon citratus as reducing and stabilising agents for nanoparticles synthesis. Data in Brief, 20, 917–926. https://doi.org/10.1016/j.dib.2018.08.081 Cardoso Valverde, T. M., Nascimento, V. M., & Silva, D. R. (2018). Plant extracts with coagulant and antimicrobial potential for water treatment. Journal of Environmental Management, 223, 623–631. https://doi.org/10.1016/j.jenvman.2018.06.072 Chaudhary, R., & Janmeda, P. (2022). Quantitative estimation of phytoconstituents in herbal extracts: An overview. Journal of Pharmacognosy and Phytochemistry, 11(1), 2257–2262. https://doi.org/10.22271/phyto.2022.v11.i1af.13850 Ewansiha, J. U., Oladosu, P., & Ndimele, P. E. (2021). Antimicrobial activity of Moringa oleifera extracts against selected bacteria from crude oil-polluted sites. Scientific African, 12, e00768. https://doi.org/10.1016/j.sciaf.2021.e00768 Ezeonu, C. S., & Ejikeme, P. M. (2016). Qualitative and quantitative determination of phytochemicals in aqueous extract of Chromolaena odorata leaves. Journal of Emerging Trends in Engineering and Applied Sciences, 7(5), 318–321. Fleming, M. C., Hester, V., Allison, B. J., Foster, M. C., Nofziger, D., & Joyner, P. M. (2018). Immunomodulatory and antibacterial properties of the Chumash medicinal plant Trichostema lanatum. Medicines, 5(2), 25. https://doi.org/10.3390/medicines5020025 Gupta, M., Sharma, S., & Sharma, R. A. (2019). Qualitative and quantitative determination of phytochemicals in selected medicinal plants. Journal of Drug Delivery and Therapeutics, 9(4-A), 748–753. https://doi.org/10.22270/jddt.v9i4-A.3473 Hussain, S., Javed, W., Tajammal, A., Khalid, M., Rasool, N., Riaz, M., & Shah, S. A. A. (2023). Synergistic antibacterial screening of Cymbopogon citratus and Azadirachta indica: Phytochemical profiling and antioxidant and hemolytic activities. ACS Omega, 8(19), 16600–16611. https://doi.org/10.1021/acsomega.3c01563 Ihegboro, G. O., Akhigbe, R. E., & Esomonu, U. G. (2022). Antibacterial activity and microbial load assessment of aqueous extracts of selected medicinal plants. Asian Journal of Research in Infectious Diseases, 10(4), 25–33. https://doi.org/10.9734/ajrid/2022/v10i4229 Kaba, T. M., & Goroya, K. G. (2019). Determination of concentration of some essential and heavy metals in roots of Moringa stenopetala using flame atomic absorption spectroscopy. Journal of Medicinal Plants Research, 13(4), 89–95. https://doi.org/10.5897/JMPR2019.6839 Karnena, M. K., & Saritha, M. (2022). Review on the potential of plant-based coagulants in water and wastewater treatment. Materials Today: Proceedings, 56, 197–202. https://doi.org/10.1016/j.matpr.2022.01.099 Nnadi, N. E., Akpan, O. E., & Wokoma, C. U. (2022). Crude oil pollution and its impact on water quality in the Niger Delta: A review. Environmental Pollution and Climate Change, 6(1), 1–10. https://doi.org/10.4172/2573-458X.1000287 Olawuwo, B. O., Ajiboye, T. O., & Omisore, O. O. (2022). Proximate and mineral analysis of some selected medicinal plants used in Nigeria. African Journal of Biomedical Research, 25(3), 395–402. Othmani, A., Bouaziz, M., & Marzougui, N. (2020). Valorization of Moringa leaves: Functional properties and phytochemical profile. Journal of Food Measurement and Characterization, 14, 1827–1834. https://doi.org/10.1007/s11694-020-00414-y Owodunni, A. A., & Ismail, A. F. (2021). A review on the use of plant-based natural coagulants in water and wastewater treatment. Water-Energy Nexus, 4, 144–155. https://doi.org/10.1016/j.wen.2021.04.001 Pandey, A., Joshi, S., & Rana, S. (2020). Plant-based coagulants for water treatment: An overview of recent research. Environmental Technology & Innovation, 19, https://doi.org/10.1016/j.eti.2020.100829 Su, J., Li, S., Yang, Z., & Xie, W. (2017). Skin irritation and sensitization potential of herbal extracts used in cosmetics. Regulatory Toxicology and Pharmacology, 89, 144–149. https://doi.org/10.1016/j.yrtph.2017.07.014 Suriya, S., Uthirapandi, V., Chelladurai, I., & Jeyaprakash, K. (2023). A preliminary phytochemical and antimicrobial analysis on Senna alata (L.) Roxb. (Leguminosae). International Journal of Botany Studies, 8(6), 10–15. Ukhurebor, K. E., Ogbeide, S. E., & Achadu, O. J. (2021). Petroleum hydrocarbon pollution and remediation techniques: A review. Journal of Environmental Management, 283, 111982. https://doi.org/10.1016/j.jenvman.2021.111982 Ukwueze, C. C., Udeogu, I. C., & Okeke, M. O. (2019). Variations in secondary metabolite composition of Moringa oleifera leaves under different environmental conditions. Journal of Medicinal Plants Research, 13(5), 112–120. https://doi.org/10.5897/JMPR2019.6837 Ukwueze, C. K., Okogwu, O. I., Ebem, E. C., Nwonumara, G. N., & Nwodo, J. N. (2019). Evaluation of the influence of geographical location on phytochemical composition of Moringa oleifera seeds. World Applied Sciences Journal, 37(3), 196–201. https://doi.org/10.5829/idosi.wasj.2019.196.201 Uzama, D., Abdullahi, S., Okeniyi, S. O., & Adeyemi, M. A. (2018). Antimicrobial activities and phytochemical properties of Ficus exasperata root extracts. Journal of Chemical Society of Nigeria, 43(2), 198–204.

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