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Morphological Variability, Character Association, Phytochemical Composition and DNA Yield in Soybean Varieties, Glycine Max

Adewusi, OF Fayeun, LS and Ikwuka, EC

Abstract

This study was carried out on the “Morphological variability, character association, Phytochemical composition and DNA yield in Soybean varieties (Glycine max (L.) Merrill)”. The soybean varieties used for this study were obtained from the soybean germplasm bank of International Institute of Tropical Agriculture (IITA), Ibadan, Oyo State, Nigeria. The experiment was carried out at the Teaching and Research Farm, Federal University of Technology, Akure, Ondo State, Nigeria. The experiment was laid out in a randomized complete block design with two replications. Data were collected on eleven agronomic characters. SAS version 9.2 was employed for all statistical analysis. The DNA extraction was carried out using NIMR kit I and Sodium dodecyl sulfate (SDS) protocols. The DNA yield and quality were determined using spectrophotometric approach and agarose gel. The analysis of variance revealed that the soybean varieties recorded significant differences for some of the characters considered at 1% and 5% probability implying that they were from diverse origin. The correlation coefficient between seed yield demonstrated a positive significant correlation with Plant height at flowering, Plant height at maturity, Number of pods, Pod length, and Total pod weight. The phytochemical composition revealed varying levels of Phytate (25.54 to 33.78 mg/g), Saponin (2.05 to 3.60%), Tannin (0.05 to 0.23mg/g) and Oxalate (0.90 to 2.79%) among the soybean varieties. The DNA yield for the NIMR kit I ranged from 625.00 to 5625.00 ng. 60% of the soybean varieties recorded DNA yield above average. For the SDS protocol, the DNA yield ranged from 3750.00 to 20312.00 ng; about 40% of the soybean varieties recorded DNA yield above average. From the study, it can be concluded that the soybean varieties were from diverse origins indicating that hybridization among them would result in good progenies. The characters with high positive correlation with seed

Keywords

MorphologicalCharacter associationPhytochemicalSoybeanVarietiesDNA yield

References

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