Submit your papersSubmit Now
For Enquiries: [email protected]
IIARD LogoIIARD

Synthesis of Zinc Oxide Nanoparticles Using Colocasia Esculenta Leaves Extract and Antimicrobial Studies of White Yam Pathogens

Ugosor, P.T, Shausu, A.A

Abstract

Zinc oxide nanoparticles (ZnO NPs) were synthesized by a green method using aqueous leaves extract of Colocasia esculenta, L and characterized by UV-Visible, XRD, SEM, EDX and FTIR. The data obtained from the zone of inhibition (mm) was analyzed using the statistical package for social Sciences, SPSS Version 20. Results were reported as Mean ± SD. The statistical difference between more than 2 groups of data was evaluated using ANOVA with LSD post hoc test. Differences between means were considered significant at p < 0.05. The study revealed that the maximum rate of synthesis could be achieved with 0.50 moldm-3 ZnO solution at 90 oC in 5 hours. Well segregated wurtzite hexagonal crystalline ZnO NPs with average crystallite size of 10 nm, ranging from 9.85 nm - 10.12 nm were obtained. FTIR spectra of the extract and the synthesized ZnO NPs revealed reducing agents such as phenolic groups as well as capping and stabilising agents such as amines, peptides and amides groups. The biosynthesized ZnO NPs exhibited antimicrobial action in a dose-dependent manner against five white yam pathogenic fungi: Aspergillus niger, Aspergillus flavus, Botryodioplodia theobromae, Phizopus stolenifera and Fusarium oxysporum as well as three bacteria: Klebsiella oxytoca, Serratia marcenscens and Pseudomonas aeruginosa. The biosynthesized ZnO NPs exhibited effective to moderately effective inhibition ranging from 89.95 % to 32.22 % on the test organisms. The ZnO NPs favourably with standard antifungal (Ketoconazole) and antibacterial (Septrim) agents. The ZnO NPs holds great potential in reducing postharvest white yam tuber rot and many other related agricultural products losses as well as a source of active ingredients for antimicrobial drug formulation and development.

Keywords

Phytochemicalsnanotechnologybiosynthesispathogensantimicrobial activity.

References

1. Ahmed, S.A., Taia, A., Ahmad, O.B., Samy, S., Abir, M.H. (2022). A.Green Synthesis and Characterization of ZnO Nanoparticles Using Pelargonium odoratissimum(L.) Aqueous Leaf Extract and Their Antioxidant, Antibacterial and Anti-inflammatory Activities. Antioxidants (Basel).2022;(8):1444.DOI:10.3390/antiox11081444.Available:https://doi.org/10.423 6/fns.2017.87051. Amin, G., Asif, M.H., Zainelabidin, A., Zaman, S., Nur, O., Willander, M. (2011). Influence of Ph, precursor concentration, growth time, and temperature on the morphology of Zno nanostructures grown by the hydrothermal method. Journal of Nanomaterials. 2011;269692. Divya, M.J., Sowmia, C., Joona, K., Dhanya, K.P. (2013). Synthesis of zinc oxide nanoparticle from Hibiscus rosa-sinensis leaf extract and investigation of its antimicrobial activity. Res; 2013. Eleazu, C.0. (2016). Characterization of the natural products in cocoyam (Colocasia esculenta) using GC-MS. Pharmaceutical Biology. 2016;54(12):2880-2885. Elumalai, K., Velmurugan, S. (2015). Green synthesis, characterization and antimicrobial activities of zinc oxide nanoparticles from the leaf extract of Azadirachta indica (L). Applied Surface Science. 2015;345:329-36. Jha, A.K., Kumar, V., Prasad, K. (2011). Biosynthesis of metal and oxide nanoparticles using orange juice. J. Bionanoscience. 2011;5(2):162–166. Available: http://dx.doi.org/10.1166/jbns.2011.1053. Farjana, R., Md Abdul M.P., Md Abu, B.S, Muhammad, S.B, Md. Aminul, H., Beauty, A., Rimi, R., Md. Anamul, H., Royhan, A.K.M. (2022). Green synthesis of ZnO nanoparticles using Cocos nucifera leaf extract: Characterization, antimicrobial, antioxidant, and photocatalytic activity;2022. Available: https//doi.org/10.11012020.1 0.27.514023. Jayanta, K. B. (2013). Synthesis and characteriztion of zno nanoparticles. a master of science dissertation of the department of physics. National Institute of Technology, Rourkela, Orissa, India; 2020. Josef, J., Katarina, K. (2015). Application of nanotechnology in agriculture and food industry, its prospects and risks. Ecol chem Eng s. 2015;22(3):321-361. Kharissova, O.V., Dias, H.V.R., Kharisov, B.I, Perez, B.O, Perez, V.M.J. (201). The greener synthesis of nanoparticles Trends in Biotechnology. 2013;31(4):240-48. Kumar et al. (2019). Zinc oxide nanoparticles: A review of their antimicrobial activity and applications in medicine. Journal of Nanoparticle Research, 21(10). Lakshmi, J.V., Sharath, R., Chandraprabha, M.N., Neelufar, E., Hazra, Abhishikta, Patra, Malyasree Synthesis, characterization and evaluation of antimicrobial activity of zincoxide nanoparticles. J. Biochem. Technology. 2012;3(5):S151–S154. Mittal, A.K., Chisti, Y., Banerjee. U.C. (2013). Synthesis of metallic nanoparticles using plant extracts Biotechnol Adv; 2013. DOI: 10.1016/j.biotechadv.2013.01.003. Moloto, N., Revaprasadu, N., Musetha, P.L, Moloto, M.J. (2009). The effect of precursor concentration, temperature and capping group on the morphology of CdS nanoparticles. Journal of Nanoscience and Nanotechnology. 2009;9:4760-66. Nakade, D.B., Mahseh, S.K., Kiran, N.P., Vinayak, S.M. (2013) Phytochemical screening and Antibacterial Activity of Western Region wild leaf Colocasea esculenta. International Research Journal of Biological Science. 2013;2(10):1-6. Official Methods of Analysis of AOAC International. (2023). 22nd ed. AOAC International, Gathersburg, MD, USA, Official Methods, 2023.005. Padil, V.V.T, Cernik, M. (2013). Green synthesis of copper oxide nanoparticles using gum karaya as biotemplate and their antibacterial application. International Journal of Nanomedicine. 2013;8:889-98. Parveen, K., Banse, V., Ledwani, L (2015). Green synthesis of nanoparticles: Their advantages and disadvantages 2nd International Conference on Emerging Technologies: Micro to Nano; 2015 (ETMN-2015). DOI: 10.1063/1.4945168. Pritha, C., Papiya, D., Sudeshna, C., Bohniskilda, C., Jayantihi, A. (2015). Cytotoxicity and antimicrobial activity of Colocasea esculenta. Journal of Chemical and Pharmaceutical Research. 2015;7(12):627-635. Priyatharesini, P.I., Ganesamoorthy, R., Sudha, R. (2020). Synthesis of zinc oxide nanoparticle using Cocos nucifera male flower extract and analysis of their antimicrobial Activity. J of Pharm and Tech. 2020;13:2151- 2154. Rad, S.S., Sani, A.M., Mohseni, S. (2019). Biosynthesis, characterization and antimicrobial activities of zinc oxide nanoparticles from leaf extract of Mentha pulegium (L.). Microbial Pathogenesis. 2019;131(2019):239–245. Rahayu, V., Wonoputri, V., Nad Samadhi, T.W. (2011). Plant extract-assisted biosynthesis of zinc oxide nanoparticles and their antibacterial applications. Material Science and Engineering. 2020;823;012-036. Rajiv, P., Rajeshwari, S., Venckatesh, R.. Rambutan. (2013). Peels promoted biomimetic synthesis of bioinspired zinc oxide nanochains for biomedical applications. Spectrochim. Acta Part A Mol.Biomol.Spectros. 2013;112:384–387. Available:http://dx.doi.org/10.1016/j.saa.2014.08.022. Ramesh, P., Rajendran, A., Meenakshisundaram, M. (2014). Green synthesis of zinc oxide nanoparticles using flower extract Cassia auriculata. J NS NT. 2014;1(1):41–45. ISSN 2279 –0381. Salam, A.H, Sivaraj, R., Venckatesh, R. (2014). Green synthesis and characterization of zinc oxide nanoparticles from Ocimum basilicum, L. var. purpurascens, Benth.- Lamiaceae leaf extract. Mater.Lett. 2014;131:16–18. Available: http://dx.doi.org/10.1016/j.matlet.2014.05.033. Sadeghi, A., et al. (2019). "Antimicrobial effects of nanoparticles: A review." Journal of Microbial and Biochemical Technology. 10 (12): 12 -20. Sangeetha, G., Rajeshwari, S., Venckatesh, R. (2011). Green synthesis of zinc oxide nanoparticles by aloe barbadensis miller leaf extract: Structure and optical properties. Mater. Res. Bull. 2011;46:2560–2566. Sathishkumar, G., Rajkuberan, C., Manikandan, K., Prabukumar, S., DanielJohn, J., Sivaramakrishnan, S. (2017). Facile biosynthesis of antimicrobial zinc oxide (ZnO) Nano flakes using leaf extract of Couroupitaguianensis.Aubl, Mater. Lett. 2017;188:383–386. Shiriki, D., Ubwa, S.T., Yusufu, M.I., Shambe, T. (2019). Extraction methods and inhibition studies of ten plant extracts on nine yam rot pathogenic microorganisms. Food and Nutrition Sciences; 2019. Available:https://doi.org10.4236/fns.2019. Shiriki, D., Obochi, G.O., Eke, M.O, Shambe T. (2017). Postharvest Loss Control: Synergistic Plants Extract Inhibition of Ten Microbial Yam Rot Organisms. Journal of food science and nutrition.2017;8(7):25-732. Sibiya, P.N, Moloto, M.J. (2014). Effect of precursor concentration and pH on the shape and size of starch capped silver selenide (Ag2Se) nanoparticles. Chalcogenide Letters. 2014;11(11):577-88. Terngu, P.U., Anhwange, A., Okibe, F.G and Dooshima, S. (2024). Green synthesis of Zinc Oxide Naniparticles using Colocasia esculenta Tuber Peel Extract and Antimicrobial Studies Aganist White Yam Pathigens. Asian J. Food Res. & Nutri., vol. 3, no. 2, pp. 306-319, 2024; Article no.AJFRN.116492. Terngu, P.U., Anhwange, A., Okibe, F.G and Dooshima, S. (2024). Isolation and Identification of Pathogens Associated with Posthharvest White Yam (Dioscorea rotundata L) Tuber Rot. Asian J. Food Res. & Nutri., 3(3): 689-701. Vijayakumar, S., Vinoj, G., Malaikozhunddan, B., Shanthi, S., Vaseeharan, B. (2015). Plectranthus amboinicus leaf extract mediated synthesis of zinc oxide nanoparticles and its control of methacillin resistant Staphylococus aureus biofilm and blood sucking mosquito larva. Spectrochim. Acta Part Amol. Biomol. Spectrose. 2015; 137: 886-891. Available: http://dx.doi.org/10.1016/j.saa.2014.08.064. Wang, J.K. (2019). Taro-a review of Colocasia esculenta and its potentials. Journal o

More Articles from INTERNATIONAL JOURNAL OF CHEMISTRY AND CHEMICAL PROCESSES

Toxic Effect of High Doses of Monosodium Glutamate on the Kidney Histology of Adult Wistar Rat

Author: Idehen, I.C., Dic-Ijiewere, E.O., Airhomwanbor, K.O., Ogun, F. E. Okparaku, S. O., Ibhawaegbele, S.O. & Igwe R. M. N.

Bacterioloigical Assesment of Water from Otamiri River in Owerri Imo State

Author: Ogah, J. O. & Ogah R. O. and Chemistry/Biochemistry Department Fedreal Polytechnic Nekede Owerri Imo State.