Sequence Analysis of Partial Coding Sequence of Growth Hormone (GH) Gene of Yankasa and Balami Sheep Breeds
Abstract
This study was undertaken to identify GH gene polymorphism in Yankasa and Balami sheep breeds. Blood samples of about 2mls were collected from 40 randomly selected sheep (20 Yankasa and 20 Balami sheep) and Deoxyribonucleic acid was extracted from 200 ?L of blood. The partial coding sequence of growth hormone gene was amplified by Polymerase Chain Reaction (PCR) using published primer set. Polymerase Chain Reaction–Restriction Fragment Length Polymorphism (PCR-RFLP) technique was used to detect polymorphism of growth hormone gene by using restriction enzyme (Hae III). The PCR products were sequenced by dideoxy sequencing in ABI® 3100 automated DNA sequencer. The obtained sequences were trimmed to 311 base pair using BioEdit® software while alignment was carried out using Basic Local Alignment Search Tool (BLASTn) and multiple alignment using fast fourier transform (MAFFT) software programmes. The PCR-RFLP Analysis revealed that there was no polymorphism in the partial coding sequence of growth hormone gene of both breeds. This was also confirmed by the result of alignment of the sequence. The BLASTn result revealed a high similarity between the nucleotide sequence of growth hormone gene of Yankasa and Balami sheep with other sheep breeds. The nucleotide sequences of Yankasa and Balami sheep were submitted to GenBank and have the accession numbers: MT722066.1 and MT722061.1 respectively. In conclusion, the present result revealed the absence of polymorphism at partial coding sequence of growth hormone gene of Yankasa and Balami sheep within the studied population implying the region is conserved.
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