Effects of Portulaca oleracea Stem Extract on Male Reproduction in Albino Rats ( Rattus norvegicus )

Wobo; Ekemini Macaulay

doi:10.56201/jbgr.v10.no2.2024.pg22.37

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Effects of Portulaca oleracea Stem Extract on Male Reproduction in Albino Rats ( Rattus norvegicus )

Wobo, Ekemini Macaulay

Abstract

Portulaca oleracea (Purselane) is a green leafy vegetable that is said to have some health promoting nutrients , however data on the safety and toxicology profile are scarce hence this study. The aim of this study was to evaluate the reproductive effect and toxicological profile of methanol stem extracts of Portulaca oleracea using albino rats as model. Fresh stem of Portulaca oleracea were collected and dried at room temperature to constant weight over 6 weeks, the dry stem weighed and grounded to fine powder. Methanol solvent (1.5L) was used and successive solvent extraction by cold maceration was done for 72hours. It was filtered with a filter paper and filtrate obtained, concentrated with rotary evaporator, transferred to evaporating dish and dried over a water bath. 82 albino rats were used: 12 for the acute toxicity study and 64 for the experimental study proper. Animals were acclimatized for 2 weeks before the study. In the experiment proper, animals were divided into four groups of 16 animals each. Group A: (control) received 1.5mb of clean water, Group B: 125mg/kg body weight of extract, Group C: 250mg/kg of extract, Group

References

Akaneme F. I. (2008). Identification and preliminary phytochemical analysis of herbs that can arrest threatened miscarriage in Orba and Nsukka towns of Enugu state. African Journal of Biotechnology, 7 (1): 6-11. Nayaka, H.B., Londonkar, R L. and AndumeshA, M.K. (2014). International Journal of Pharmacy and Pharmaceutical Sciences 6(5):86-89. Review. Journal of Pharmacy Research 2011,4(9),3044-3048. Foster, S. and J. A. Duke. 2000. A Field Guide to Medicinal Plants and Herbs. 2nd Ed. Boston: Houghton Mifflin Company Kagbo, H. D. & Obinna, V. C. (2017). Antifertility effect of Costus lucanuscianus stem extract in male albino rats. International Journal of Science and Technology, 5(12): 119 – 123. Chiej R.(1988). Purslane: medicinal plants. The Macdonald Encyclopoedia of Medicinal Plants. ISBN No. 0-356-10541-5 (hb), -10542-3 (pb). Rashed A.N, Afifi F.U. & Disi A.M.(2003). Simple evaluation of the wound healing activity of a crude extract of Portulaca oleracea L. (growing in Jordan) in Mus musculus JVI- Journal of Ethnopharmacology; s88(2-3):131–136. Reece, W. O. (2015). male Reproduction in mammals. In W. O. Reece, H. H. Erickson, J. P. Golff & E. E. Uemura (Eds). Duke’s physiology of domestic animals (13th ed., pp: 654-669). Cornell: University press. Sharpe, R. M., Maddocks, S. & Kerr, J. B. (1990). Cell-cell interactions in the control of spermatogenesis as studied using leydig cell destruction and testosterone replacement. Developmental Dynamic, 188(1): 3-20. Simopoulos A.P, Norman H.A, Gillaspy J.E. (1995). Purslane in human nutrition and its potential for world agriculture. World Review of Nutrition and Dietetics;77:47–74. Walker, W. H. (2009). Molecular mechanisms of testosterone action in spermatogenesis. Steroids, 74(7):602-607. http:// dx.doi.org/10.1016/j.sterioids.2008.11.017. https://doi.org/10.4161/spmg.1.2.16956 Zhang, F. P., Pararainen, T. Poutanen, M., Toppari, J. & Huhtaniemi, I. (2003). The low gonadotropin-independent constitutive production of testicular testosterone is sufficient to maintain spermatogenesis. PNAS, 100 (23): 136

Effects of Portulaca oleracea Stem Extract on Male Reproduction in Albino Rats ( Rattus norvegicus )

Abstract

References

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