Control of Post-Harvest Bacterial Soft Rot of Potato Using Aqueous Fruit Extract of Christmas Melon ( Laganaria breviflorus )

Ivoke; M.A.; Abraham; P.; Oluro; C.O.; Yohanna; H.

doi:10.56201/ijaes.v10.no6.2024.pg56.65

📄 Download Paper

Control of Post-Harvest Bacterial Soft Rot of Potato Using Aqueous Fruit Extract of Christmas Melon ( Laganaria breviflorus )

Ivoke, M.A. , Abraham, P. , Oluro, C.O. and Yohanna, H.

Abstract

Managing the post-harvest bacterial soft rot of potato with chemicals can lead to severe health issues. During this study, an attempt was made to use aqueous Christmas melon (Laganaria breviflorus) fruit extract to control bacteria soft rot of potato. The experiment was carried out in the Pathology Laboratory of the Federal College of Horticulture, Dadin-kowa, Gombe, Nigeria in 2022, and laid in a Completely Randomized Design with four replications. The treatments used were the different concentrations of the aqueous Christmas melon fruit extract comprising (stock solution), (80% concentrated CMFE), (60% concentrated CMFE), (40% concentrated CMFE), (20% concentrated CMFE) and Control (pathogen only without treatment). The bacterium was isolated from infected potato tuber and was plated on Nutrient Agar (NA). Pathogenicity test was carried out to confirm the pathogen before the In-vivo study. Peeled fruits of L. breviflorus were fermented for seven days in distilled water. The inoculated tubers were treated with aqueous Christmas melon fruit extract and incubated for six days at 27°C. Graphpad Prism version 9 software package was used for the analysis. The results revealed that L. breviflorus aqueous fruit extracts did not protect the potato tuber against bacteria soft rot, and therefore cannot serve as substitute for synthetic pesticide in controlling bacteria soft rot of potato caused by Pectobacterium carotovora ssp carotovora. Further study is recommended to be carried out using organic solvents such as n-hexane, methanol, etc to extract the botanical pesticide from Christmas melon fruit as there might be possibility of getting a positive result.

Keywords

Aqueous extractErwinia carotovoraLaganaria breviflorusPathogenicity

References

Badmos, A and Mamood, Y., 2021. Effect of Christmas Melon (Laganaria Breviflorus) extract on toxigenic Mycoflora Isolated from Stored Unpolished Rice sold in major Markets in Abeokuta, Nigeria. Turkish Journal of Agriculture - Food Science and Technology. 2021; 9(sp): 2680-2686. Bdliya B.S., Dahiru B. 2006. Efficacy of some plant extracts on the control of potato tuber soft rot caused by Erwinia carotovorassp. carotovora. Journal of Plant Protection Res. 46 (3): 285–294. Bdliya, B. S. and Langerfeld, E. 2005b. Soft rot and Blackleg [Erwinia carotovora ssp. atroseptica (Van Hall) Dye] of potato as affected by inoculum density and variety. Nigerian Journal of Plant Protection, 22:65–75. Czajkowski, R., Pérombelon, M.C.M., Van Veen, J.A., Van Der Wolf, J.M., 2011. Control of blackleg and tuber soft rot of potato caused by Pectobacterium and Dickeya species: a review. Plant Pathol. 60, 999–1013. Des Essarts, R.Y., Cigna, J., Quêtu-Laurent, A., Caron, A., Munier, E., Beury-Cirou, A., Hélias, V., Faure, D., 2016. Biocontrol of the potato blackleg and soft rot diseases caused by Dickeya dianthicola. Appl. Environ. Microbiol. 82, 268–278. Deshpande R.R, Kale A.A and Ruikar AD 2011. Antimicrobial Activity Of different extracts of Juglans Regia L. against Oral Microflora. International Journal of Pharmacy and Pharmaceutical Science. 2011; 03:200-201. Gracia-Garza, J.A., Blom, T.J., Brown, W., Allen, W., 2002. Pre-and post-plant applications of copper-based compounds to control Erwinia soft rot of calla lilies. Can. Journal of Plant Pathology 24, 274–280. Gomez, K. A. and Gomez, A. A. 1984. Statistical Procedure for Agriculitural Research. 2nd (ed.) Wiley, 680 pp Hajhamed, A.A., Sayed, W.M.A.E., Yazied, A.A.E., Ghaffar,N.Y.A.E., 2007. Suppression of bacterial soft rot disease of potato. Egypt. Journal of Phytopathology 35, 69–80. Jess, S., Kildea, S., Moody, A., Rennick, G., Murchie, A.K., Cooke, L.R., 2014. European Union policy on pesticides: implications for agriculture in Ireland. Pest Manag. Sci. 70, 1646– Kowal, J. M. and Knabe, D., 1972. Agro-climatological Atlas of the Northern States of Nigeria. Ahmadu Bello University Press, Samaru 1972, 112 pp. Las Llagas, MCD, Santiago L, Ramos, J.D., 2014. Antibacterial activity of crude ethanolic extract and solvent fractions of Ficus psedopalma Blanco leaves. Asian Pacific Journal of Tropical Disease. 2014; 4(5):367-371. Rahman S, Anowar P.R and Khan I.M 2011. Antibacterial activity of natural spices on multiple drug resistant Escherichia coli isolated from drinking water, Bangladesh. Annals of Clinical Microbiology and Antimicrobials. 2011; 10(1):10. Sahi, S.T., Ghazanfar, M.U., Tahir, M.N., 2007. Physiology of Erwinia associated with black leg potato. Pakistan. Journal of Agricultural Science 44, 259–265. Thompson, A, Dilruba M, Nadia A, Rebecca CJ, Emma C, Tim C.C., 2013. Comparison of the antibacterial activity of essential oils and extracts of medicinal and culinary herbs to investigate potential new treatments for irritable bowel syndrome, BMC Complementary and Alternative Medicine. 2013; 13(1):338. Vasinauskiene, M., Radusiene, J., Zitikaite, I., Surviliene, E., 2006. Antibacterial activities of essential oils from aromatic and medicinal plants against growth of phytopathogenic bacteria. Agron. Res. 4, 437–440.

Control of Post-Harvest Bacterial Soft Rot of Potato Using Aqueous Fruit Extract of Christmas Melon ( Laganaria breviflorus )

Abstract

Keywords

References

More Articles from INTERNATIONAL JOURNAL OF AGRICULTURAL AND EARTH SCIENCE