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Isolation And Identification of Bacteria from Infected Burns with Pathological Changes and Evaluation the Antibacterial Effects of Lactobacillus Rhamnosus and Lactobacillus Buchnerri Filtrates

Noora A. Hassan

Abstract

This study was done for evaluation the antimicrobial effects of Lactobacillus rhamnosus and Lactobacillus buchnerri filtrates on the main causes of burn infections with studying the histopathology of this burn. One hundred thirty-five swabs were taken from 135 burn cases from patients come to clinics in Baghdad. These swabs were cultured on primary media, then biochemical tests were done for identification of these bacteria. Lactobacillus filtrates was prepared. The antimicrobial susceptibility test for this filtrate was done by using agar well diffusion, also antibiotic susceptibility test was done for many antibiotics against bacterial isolates. The slices revealed the epithelium layer, the top layer of skin, and the dermal layer, the middle layer, both of which were destroyed. The presence of bacteria is confirmed by the significant infiltration of leukocytes. This was consistent with what was seen visually, namely the ulceration of the burn region and the presence of pus. The current results showed that Pseudomonas aeruginosa was the most prevalent isolates from infected burns followed by E. coli. The results of antibiotic sensitivity test showed that most isolates were MDR to most antibiotics, While the Lactobacillus filtrates showed a high antimicrobial effectivity against all bacteria. In conclusion, Pseudomonas aeruginosa was most predominant bacteria isolated from burns, also other bacteria were isolated in significant percentages which showed high susceptibility to lactobacillus filtrates.

Keywords

LactobacillusBurnsBacteria

References

1. Food and Health Agriculture Organization of United Nations and World Health Organization. Guidelines for the evaluation of probiotics in food. Joint FAO/WHO Working Group Report on Drafting Guidelines for the Evaluation of Probiotics in Food. 2002. [Accessed August 31, 2009]. 2. Naidu AS, Bidlack WR, Clemens RA. Probiotic spectra of Lactic Acid Bacteria(LAB).Crit. Rev Food Sci Nutr. 1999;38:13–126. 3. Mazza G. Functional Food, Biochemical and Processing Aspects. Boca Raton, FL: Taylor and Francis Gp. LLC; 1998. pp. 357–374. 4. Mc-Cracken BJ, Gaskins HR. Probiotics and the immune system. In: Tannok GW, editor. probiotics: a critical review. Norfolk, Va: Horizon Scientific Press; 1999. pp. 85–111. 5. Jijon H, Backer J, Diaz H, et al. DNA from probiotic bacteria modulates murine and human epithelial and immune function. Gastroenterology. 2004;126:1358–1373. 6. Madsen K, Cornish A, Soper P, et al. Probiotic bacteria enhance murine and human intestinal epithelial barrier function. Gastroenterology. 2001;121:580–591. 7. Kalliomaki M, Walker WA. Physiologic and pathologic interactions of bacteria with gastrointestinal epithelium. Gastroenterol Clin North Am. 2005;34:383–399. 8. Andreu A, Stapleton AE, Fennel CL, Hillier SL, Stam WE. Hama agglutination, adherence and surface properties of vaginal Lactobacillus species. J Infect Dis. 1995;171:1237–1240. 9. Vallor AC, Antonio MAD, Hawes SE, Hiller SL. Factors associated with acquisition of, or persistent colonization by vaginal Lactobacilli, role of hydrogen peroxide production. J Infect Dis. 2001;184:1431–1436. 10. Cadieux P, Burton J, Braunstein I, Bruce AW, et al. Lactobacillus strains and vaginal ecology. JAMA. 2002;287:1940–1941. 11. Church D, Elsayed S, Rrid O, Winston B, Lindsay R. Burn wound infections. Clin Microbiol Rev. 2006;19(2):403–434. 12. Brigham PA, McLoughlin E. Burn incidence and medical care use in the United States: estimate, tends and data sources. J Burn Care Rehabil. 1996;17:95–107. 13. Vindenes H, Bjerknes R. Microbial colonization of large wounds. Burns. 1995;21:575–579. 14. Atiyeh BS, Al-Amm CA. Immunology of burn injury. An overview. Ann Burns Fire Disasters. 2001;14:2. 15. Komolafe OO, James T, Kolongolera L. Bacteriology of burn at the Queen Elizabeth Central Hospital, Blantyre, Malaw. Burn. 2003;29:235–238. 16. Markey, B., Leonard, F., Archambault, M., Cullinane, A., & Maguire, D. (2013). Clinical veterinary microbiology e-book. Elsevier Health Sciences. 17. Perez C, Pauli M, Bezerque P. An antibiotic assay by the agar-well diffusion method. J Aotabiologiae. 1990;15:113–115. 18. NCCL. Antibiotic susceptibility methods. Clinical Laboratory Standards Institute (CLSI) 2000. 19. Balakit Huda AW. Study of some clinical, bacteriological and immunological aspects of patients with burn injury. MSc thesis. College of Medicine. University of Babylon. 2006 20. Torregrossa MV, Valentino L, Cucchiara P, Masellis M, Sucameli M. Prevention of hospital-acquired infections in the Palermo burns center. Annals of Burns and Fire Disasters. 2000;13(3) Cited by Huda A.W. Balakit. Study of some clinical, Bacteriological and Immunological Aspects of Patients with Burn injury. MSc thesis. College of Medicine. University of Babylon; 2006. 21. MacFaddin JF. Biochemical test for identification of medical bacteria. 3th ed. New York: Williams and Wilkins-Baltimore; 2000. 22. Bowler AG, Duerden BI, Armstrong DG. Wound Microbiology and associated approaches to wound management. Clin Microbiol Rev. 2001;14:244–269. 23. De Macedo JLS, Rosa SC, Castro C. Sepsis in burned patients. Revista da Sociedade Brasileria de Medicina Tropical. 2003;36(6):647–652. 24. Foster TJ. The Staphylococcus aureus “superbug” J Clin Investig. 2004;114:1693– 1696. 25. Richard P, Floch RL, Chamoux C, Pannier M. Pseudomonas aeruginosa outbreak in a burn unit: role of antimicrobials in the emergence of multiply resistant strains. J Infect Dis. 1994;170:377–383. 26. Brooks GF, Butel JS, Morse SA. Jawetz, Melnick and Aldelberg's Medical Microbiology. 23th ed. Lange Medical Books, McGraw-Hill; 2004. 27. Herek O, Ozturkk H, Ozyurt M, Albay A, Cetinkursun S. Effects of treatment with immunoglobulin on bacterial translocation in burn wound infection. Ann Burns Fire Disasters. 2000;13(1) . 28. Anca, F.;T.Emma, and B. Anca, 2019. Antibiotic resistance profiling of pathogenic Enterobacteriaceaefrom Cluj-Napoca, Romania. GERMS.9(1): 17-27. 29. AL-Hamdani, H. andA.AL-Hashimy . 2020. Molecular detection of urec, hpma, rsba and mrpa genes of proteus mirabilis urinary tract infection in patient with rheumatoid arthritis. Iraqi Journal of Agricultural Sciences. 51:245- 251. 30. Ciro César, R.; F.P.Monalessa, and G. Marcia,.2020. Underrated Staphylococcusspecies and their role in antimicrobial resistance spreading. Genetics and Molecular Biology. 43, 1(suppl 2), e20190065. 31. William Norbury, David N. Herndon and Celeste C. Finnerty .2016.Infection in Burns. Surg Infect(Larchmt).1;17(2):250-255.