Antibacterial Activities of Bay Leaf (Laurus nobilis) On Some Bacterial Isolates

Abstract

Laurus nobilis is one of the most well known most, frequently used plants is from Lauraccaefamily which contains up to 2,500 species that grows in the subtopics and tropics of the Mediterranean region and Indonesia. This study was supposed to investigate the antimicrobial effect of L. nobilis leaves ethanol extract on Staphylococcus aureus, Salmonella typhi and Escherichia coli. This preliminary study examined the antimicrobial effect of L. nobilis leaves ethanol extract. The method used is Agar Well diffusion for determination of the zone of inhibition and the minimal bactericidal concentration to investigate the activity of L. nobilisleaves ethanol extract at 100% concentration. The results revealed that the extract of L.nobilisleaves had antibacterial activity against Staphylococcus aureus with a zone of inhibition (16.3 ± 1.5mm), Salmonella typhi with (14.5±0.5mm) and weak antimicrobial activity against Escherichia coli (11.3±1.1mm). Also, through the minimum bactericidal concentration experiment, the L. nobilis leaves ethanol extract had activity against Staphylococcus aureus and Salmonella typhi, it killed the bacteria in all concentration. Starting from 5 x10 7 to 5 xl0 4 . But the activity on Escherichia coli just weaken concentration 5x10 7 and 10 6 . This research has concluded that the L. nobilis leaves ethanol extract exhibited a significant antimicrobial effect against Staphylococcus aureus and Salmonella typhi than Escherichia coli that is considered a kind of multidrug resistant bacteria.

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