Isolated from Soil for 
Enhanced Production of Alpha Amylase

Egbi; T.K.; Ijah U.J.J.; Emelogu; J.N.; Ahiaukwu; J.A.; Asake; O.J.; Ayegba; S.O.

doi:https://doi.org/10.56201/rjpst.v7.no1.2024.pg66.81

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Isolated from Soil for Enhanced Production of Alpha Amylase

Egbi, T.K., Ijah U.J.J., Emelogu, J.N., Ahiaukwu, J.A., Asake, O.J., Ayegba, S.O.

Abstract

Bacillus species capable of extracellular amylase production were isolated from soil samples using rice bran and wheat bran as substrates. Using starch hydrolysis plate assay test the bacterial isolates were screened and were identified using maximum zone of clearance and further characterized according to standard methods and molecular analysis.The isolates were identified as Bacillus safensis. They were used to produce ?-amylase. Bacillus safensis had maximum enzyme activity of 40.86 IU/mL and 43.87 IU/mL on rice and wheat bran respectively over the same period. Enhancement of enzyme production was done using two techniques; induction of mutation by UV irradiation and recombinant DNA technology. Six mutants each were randomly selected from each UV treatment time range. The mutant BSM

References

Aiyer, P. (2004). Effect of Carbon: Nitrogen ratio on alpha amylase production by Bacillus licheniformis SPT 27. African Journal of Biotechnology, 3, 519-522. Cheesbrough, M. (2006). District Laboratory Practice: In Tropical Countries, (Low price edition). The Press Syndicate of the University of Cambridge, pp.64. Frank, J. A., Reich, C. I., Sharma, S., Weisbaum, J. S., Wilson, B. A., & Olsen, G. J. (2008). Critical evaluation of two primers commonly used for amplification of bacterial 16S rRNA genes. Applied and Environmental Microbiology, 74(8), 2461-2470.

Isolated from Soil for Enhanced Production of Alpha Amylase

Abstract

References

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