Cardio-Protection Potential of Avocado Pear Seeds’ Extract on Ethylene Glycol-Triggered Toxicity in Wistar Rat

Abstract

Cardio - protection activity of Avocado pear seeds (APS) extract was investigated, on ethylene glycol-triggered toxicity using Wistar rat model. Designation of rodents was made to accommodate under listed administration; 1st group or control, Group 2 administered 0.1ml/kg b.w ethylene glycol only for seven days, 3rd and 4th groups administered avocado pear seed extract (100mg/kg b.w and 200mg/kg b.w morning and evening respectively) for 21 days and 0.1ml/kg b.w ethylene glycol for 7 days with continuous administration of the treatment dosage and the fifth group treated with 0.2mg/kg b.w folic acid and 0.3mg/kg b.w thiamine (morning and evening) for 21 days and 0.1ml/kg b.w ethylene glycol for 7 days with continuous administration of the treatment dosage. Cardioprotective effects of ethanolic extract of avocado pear seed was evaluated by estimating the activities of Lactate Dehydrogenase (LDH) and Creatine Kinase (CK). The effects of the extracts on biomarkers of oxidative damage (lipid peroxidation) and antioxidant enzymes namely, catalase (CAT), superoxide dismutase (SOD) and glutathione peroxidase (GPx) were also measured in the heart using homogenates of the organ.Histological sections of hearts were also prepared. Ethylene glycol elevated CK, with LDH (p< 0.05) as seen in the positive control group. Treatment by ethanolic extracts of avocado pear seeds significantly reduced the effect of ethylene glycol induced biochemical and histopathological alterations; implying the extract reduced toxic effects of ethylene glycol and has potential to stabilize cell membranes and protect the cell /tissues from more damage.

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