Establishment of the Phylogenetic Relationship Between the Black Skinned and White Skinned Snails (Archachatina marginata) in the Rainforest Zone of Nigeria
Abstract
This study evaluated the phylogenetic relationship between black skinned and white skinned snails (Archachatina marginata) in the rainforest zone of Nigeria. Fourty eight (48) Archachatinamarginata snails were assigned to two treatments using completely randomized design. The two treatmentsconsisted of twenty four (24) white skinned ecotypes and twenty four(24) black skinned ecotypes with 12 replicates each. These snails were taken to laboratory to conduct DNA analysis to evaluate the phylogenic relationship between the two snail ectotypes. The quality of DNA was assessed using gel electrophoresis. Data collected was subjected to statistical analysis using Mega 7.0, DNAsp version 5 and GENPOP software packages. The phylogenetic analysis revealed a 0.2 genetic variation and 0.8 similarities between the snail that is black and the white. From the dendogram, it was also observed that the white snails are the original ancestors of the black since the top of the tree was dominated by the white snails with the black occupying the bottom of the tree
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