Submit your papersSubmit Now
For Enquiries: [email protected]
IIARD LogoIIARD

Antioxidants-rich Nutraceutical Ameliorates Lead-induced Oxidative Stress in Albino Rats

S. Rabiu , M. G. Abubakar , D. M. Sahabi and M. A. Makusidi

Abstract

Treatment of lead toxicity is usually through aggressive approach such as chelation and bowel irrigation and these therapies are used just to promote body metal excretion and are considered less effective due multiple limitations and side effects. Therefore, the present study was conducted to evaluate the protective and curative potentials of formulated antioxidant-rich nutraceutcals against lead-induced oxidative stress in albino rats. A total of Fifty (50) albino rats of both sexes weighing 200–220 g were equally and randomly divided into two (2) groups (A and B models) of twenty five rats each. The group A was designed to study the protective effect of formulated nutraceutical against lead-induce oxidative stress. While group B was to evaluate the curative effect of same formulated nutraceutical in rats after lead intoxication. The rats in each of the models were randomly divided into five groups. Thirty (20) rats of lead treated groups were administered with different concentration of antioxidant-rich nutraceutical in both models. While 10 rats were treated with standard drug. Our findings reveals that oral administration of antioxidant- rich supplement in lead-intoxicated rats (in both models) significantly reduced blood lead levels, increased the activity of antioxidants enzymes and glutathione levels. Similarly, the levels of antioxidant minerals (Fe, Zn, Cu and Se) and vitamins (A, C and E) were boosted significantly. Concurrently, significant decreased levels of malondialdehyde were observed after administration of the antioxidant-rich supplement, hence, lowering lead-induced oxidative stress. Therefore, oral administration of antioxidant-rich supplement may invariably use as protective and as well as curative regimen to curtail lead-induced oxidative stress.

Keywords

LeadToxicityAntioxidantsNutraceuticalsOxidative stress

References

Ahmed, R., Seth, V., Pasha, S & Banerjee, B. (2000). Influence of Dietary Ginger (Zingiber officinales Rosc) on Oxidative Stress induced by Malathion in rats. Food and Chemical Toxicology, 38(5), 443–450 Anjum, M. R., Madhu, P., Reddy, K. P. & Reddy, P. S. (2017). The protective effects of zinc in Lead-induced Testicular and Epididymal Toxicity in Wistar Rats. Toxicol Ind Health,33(3):265-76. Baker, M.A., Cerniglia, G.J. & Zaman, A. (1990). A Microtiter Plate Assay for the measurement of Glutathione and Glutathione Disulfide in Large numbers of Biological Samples. Analytical Biochemistry, 190(2): 360-365. Buege, J. A. & Aust, S. D. (1978). Microsomal lipid peroxidation. In Methods in enzymology (Vol. 52, pp. 302–310). Elsevier Chen, W., Qixiao, Z. & Arjan, N. (2015). Dietary Strategies for the Treatment of Cadmium and Lead Toxicity. Nutrients 7: 552-571 Chen, Y., Shang, Y., Zhao, Y., Qiu, H., Jing-Jing Chang, J. & Zhang, H. (2015). Effects of a Food Ingredient Group on Oxidative Stress in Lead-Poisoned Mice. Integrative Med Int.49-55 Dkhil, M. A., Moneim, A. E. A. & Al-Quraishy, S. (2016).Indigofera oblongifolia Ameliorates Lead Acetate-Induced Testicular Oxidative Damage and Apoptosis in a Rat Model. Biol. TraceElem. Res. 173(2), 354–361 Flora, G., Gupta, D & Tiwari, A. (2012). Toxicity of Lead: a review with recent updates Interdisciplinary Toxicology, 5( 2) 47–58 Goodrich, K. M., Fundaro, G., Griffin, L. E., Grant, A., Hulver, M. W. & Ponder, M. A. (2012). Chronic Administration of Dietary Grape Seed Extract Increases Colonic Expression of Gut tight Junction Protein Occludin and reduces Fecal Calprotectin: a Secondary analysis of Healthy Wistar Furth Rats. Nutr. Res. 32, 787–794 Haleagrahara , N., Jackie, T., Chakravarthi, S., Rao, M. & Pasupathi, T. (2010). Protective Effects of Etlingera elatior Extract on Lead Acetate-induced changes in Oxidative Biomarkers in Bone Marrow of rats. Food Chem Toxicol.48(10):2688-94 Jawhar, L., Jaouad, E., Hamadi, F., Yassine, C. & Hanane, G. (2017) Berberis vulgaris effects on oxidative stress and liver injury in lead-intoxicated mice J Complement Integr Med 14: 1-7 Jedlinska-Krakowska, M., Bomba, G., Jakubowski, K., Rotkiewicz, T., Jana, B. & Penkowski, A. (2006).Impact of Oxidative Stress and Supplementation with Vitamins E and C on Testes Morphology in Rats. J Reprod Dev 52:203-9. Johansson, L.H. & Borg, L.A.H. (1998). A spectrophotometric Method for Determination of Catalase Activity in Small Tissue Samples. Analytical Biochemistry, 174(1): 331-336 Kharoubi, O., Slimani, M., Krouf, D., Seddik, L. & Aoues, A. (2008). Role of Wormwood (Artemisia absinthium) Extract on Oxidative Stress in Ameliorating Lead induced Hematotoxicity. Afr. J. Tradit. Complement. Altern. Med. 5:263-270. Kim, H., Jang, T., Chae, H., Choi, W., , Ha, M., Ye, B., Kim, B., Jeon, M., Kim, S. & Hong, Y. (2015). Evaluation and Management of Lead Exposure. Annals of Occupational and Environmental Medicine. 27(30). 1-9 Kordas, K. (2017).The “lead diet”: Can Dietary Approaches Prevent or Treat Lead Exposure? J. Pediatr. 185; 224-231 Lamidi, I. Y & Akefe, I. O. (2017). Mitigating Effects of Antioxidants in Lead Toxicity. Clin Pharmacol Toxicol Jour 1 (3): 1-9 Mediha, B., Kharam, S. A., Zahidqureshi, Nida, M. & Nimra. (2018). Determination of Heavy Metals Toxiccity in Blood and Health Effect . Archive of nanomedicine 1 (2): 22-28 Oboma, Y. I., Sylvanus, B., Okara, P. N., Favour, I., A Tamuno-omie, A. T. & Ibiang, O. E. (2018). Protective Effect of Combined Aqueous Extracts of Allium sativum and Zingiber officinale against Lead acetate Induced Hepatotoxicity and Testicular Damage in Rattus norvegicus. MOJ Anat & Physiol.5(5):306?313 Paglia, D. E. & Valentine, W. N. (1967). Studies on the Quantitative and Qualitative Characterization of Erythrocyte Glutathione Peroxidase. J Lab Clin Med, 70: 158-169 Patra, R. C., Swarup, D. & Dwivedi, S. K. (2001). Antioxidant effects of alpha Tocopherol, Ascorbic acid and L-Methionine on Lead induced Oxidative Stress to the Liver, Kidney and Brain in Rats. Toxicology 162, 81–88. Reddy, Y. S., Aparna, Y., Ramalaksmi, B. A. & Kumar, B. D. (2014). Lead and Trace Element Levels in Placenta, Maternal and Cord Blood: a cross-sectional pilot study. The Journal of Obstetrics and Gynaecology Research, 40 (12): 2184–2190 Rutkowski, M. & Grzegorczyk, K. (2007). Modifications of Spectrophotometric Methods for Antioxidative Vitamins Determination Convinient in Anallytical Practice. Acta sci. pol. Technol. Aliment, 6(3): 17-28 Sadhana, S., Veena, S. & Rita, P.P.(2011). Lead Toxicity, Oxidative Damage and Health Implications. A Review. Inter. J. Biotech and Mol. Biol. Res.2(13):215-221 Saidu Y., Bilbis L. S., Muhammad S. A. & Nasir M. K. (2012). Serum Lipid Profile and Antioxidant Status of Salt-Induced Hypertensive Rats Treated with an Antioxidants Rich Nutraceutical. Cameroon Journal of Experimental Biology, 8(1): 47-54. Sirivarasai, J., Kaojarern, S., Chanprasertyothin, S., Pachara, P., Krittaya, P., Aninthia T. & Krongtong, Y. (2015). Environmental Lead Exposure, Catalase Gene and Markers of Antioxidant and Oxidative Stress Relation to Hypertension: An Analysis Based on the EGAT Study. BioMed Res. Int. 1-9 Sun, Y., Oberley, L.W. & Li, Y. (1988). A simple method for clinical assay of superoxide dismutase, Clinical Chemistry, 34(3):497-500. Tariq, U., Sadiq Butt, M. & Hameed, A. (2018). Probing the Individual and Synergistic Effect of Calcium and Zinc against Lead Induced Hematological Alterations and Oxidative Stress. Ann Complement Altern Med. 1(1): 1-6 Wali, U., Saidu, Y., Ladan, M.J., Bilbis, L.S. & Ibrahim, N.D. (2013). Antioxidant Status and Lipid Profile of Diabetic Rats Treated with Antioxidant rich Locally Prepared Nutraceutical. International Journal of Diseases and Disorders, 1(2): 33-38. Zhai, Q., Narbad, A. & Chen, W. (2015). Dietary Strategies for the Treatment of Cadmium and Lead Toxicity .Nutrients 7, 552-571

More Articles from INTERNATIONAL JOURNAL OF HEALTH AND PHARMACEUTICAL RESEARCH

Antibacterial Activity of Stem Bark Extract of Boswellia Odorata Against Some Isolates of Wound Among Patients Attending Selected Hospitals in Kano Metropolis

Author: Kamal Abdulkadir Muhammad, Ismaila Ahmed, Lawan Danjuma, Raliya Sulaiman, Dauda, Isah Musa Bebeji, Zubairu Sani Ibrahim, Yahaya Ubah Yau, Binta Kabir, Muhammad, and Mukhtar Aminu Bala, Corresponding author

Prevalence and Prevention of Health Problems Among Inmates in Agodi Gate Correctional Centre, Ibadan

Author: John Betiku, a, Oluwabunmi Hannah Aremo, b, Emmanuel Olusegun Abe, a, Rachael, Omotomilayo Ajayi, c, Adenike Kaosarat Alabi, d, Kelechi Princess John, d, orcid ---