Phylogenetic Analysis and Genetic Diversity of Bacteria Strains isolated from oral cavity of Malnourished Children: Insights from Comparative Genomics
Abiodun Jacob Osatogbe, Prof. Ahmed Ali Farouq, Associate Prof. Daniel. D Attah, Prof. Sule Sahabi Manga
Abstract
Molecular Characterization of Bacteria Isolated from the Oral Cavities of In-Patient Malnourished Children At Specialist Hospital, Sokoto, Nigeria. Research conducted at Sokoto State Specialist Hospital looked into oral bacteria in malnourished children. Malnutrition stems from nutrient inefficiency, and diet influences health significantly. A microbiological method of isolation involves aseptic oral swabs for examination. Samples are collected by spinning the swab across the mouth for 15ā20 seconds. Self-sealing polythene bags transport specimen containers to the lab, ensuring accurate results. Isolation uses nutrient agar, sorbitol, and broth agars. Gram staining, biochemical testing detect nutritious microorganisms on the agar-spread plate. The slide is dried and examined under a microscope with an oil immersion objective lens. With molecular characterization of bacteria isolates from the oral cavity of the patients using PCR.
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