IIARD International Institute of Academic Research and Development

International Journal of Agriculture and Earth Science (IJAES )

E- ISSN 2489-0081
P- ISSN 2695-1894
VOL. 11 NO. 3 2025
DOI: 10.56201/ijaes.vol.11.no3.2025.pg104.113

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Sequence Analysis of Partial Coding Sequence of Growth Hormone (GH) Gene of Yankasa and Balami Sheep Breeds

Ali, Y, Ojo, OA, and Dubagari, NG

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Abstract

This study was undertaken to identify GH gene polymorphism in Yankasa and Balami sheep breeds. Blood samples of about 2mls were collected from 40 randomly selected sheep (20 Yankasa and 20 Balami sheep) and Deoxyribonucleic acid was extracted from 200 ?L of blood. The partial coding sequence of growth hormone gene was amplified by Polymerase Chain Reaction (PCR) using published primer set. Polymerase Chain Reaction–Restriction Fragment Length Polymorphism (PCR-RFLP) technique was used to detect polymorphism of growth hormone gene by using restriction enzyme (Hae III). The PCR products were sequenced by dideoxy sequencing in ABI® 3100 automated DNA sequencer. The obtained sequences were trimmed to 311 base pair using BioEdit® software while alignment was carried out using Basic Local Alignment Search Tool (BLASTn) and multiple alignment using fast fourier transform (MAFFT) software programmes. The PCR-RFLP Analysis revealed that there was no polymorphism in the partial coding sequence of growth hormone gene of both breeds. This was also confirmed by the result of alignment of the sequence. The BLASTn result revealed a high similarity between the nucleotide sequence of growth hormone gene of Yankasa and Balami sheep with other sheep breeds. The nucleotide sequences of Yankasa and Balami sheep were submitted to GenBank and have the accession numbers: MT722066.1 and MT722061.1 respectively. In conclusion, the present result revealed the absence of polymorphism at partial coding sequence of growth hormone gene of Yankasa and Balami sheep within the studied population implying the region is conserved.

keywords:

Partial coding sequence, Yankasa sheep, Balami sheep, Growth hormone gene, Polymorphism

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References:

Al-Salihi, A., Al-Saadi, B. and Al-Anbari, N. (2017). Genotypes relationship of Growth hormone
gene polymorphism with some productive and reproductive trait in Awassi sheep. Journal
of Biotechnology and Resource Center, 11.
Amiri, S., Bayrem, J., Mohamed, A.F., Hajer, J., Rekik, B. and Abderrahmane, B.G. (2018).
Assessment of growth hormone gene polymorphism effects on reproductive traits in
Holstein dairy cattle in Tunisia. Archive of Animal Breed, 61(4): 481-489.
https://doi.org/10.5194/aab-61-
Anwar, S. (2017). Association of growth hormone gene polymorphism with quantitative
characteristics of thin tailed sheep using PCR-RFLP in Jambi province. African Journal of
Biotechnology, 16(20):1159-1167. https://doi.org/10.5897/AJB2016.15783
Ayuk, J. and Sheppard, M.C. (2006). Growth hormone and its disorders, Postgraduate Medicine,
82: 24–30.
Castle, J.C. (2011). SNPs occur in regions with less Genomic Sequence Conservation. Public
Library of Science ONE, 6(66): e20660. doi:10.1371/journal.pone.0020660
Cauveri, D., Sivaselvam, S.N., Karthickeyan, S.M.K., Tirumurugaan, K.G., Kumanan, K. and
Venkataramanan, R. (2016). Single nucleotide polymorphisms in Growth Hormone gene
associated with growth traits in Nilagiri sheep of Tamil Nadu. International Journal of
Science, Environment and Technology, Vol. 5, No 6, 2016, 4097 – 4103.
Chen, Y. Wang, Y., He, S. and Zhu, Z. (2004). Cloning and sequencing of the growth hormone
gene of large yellow croaker and its phylogenetic significance. Biochemical Genetics, 42:
365–375.
El-Komy, S.M., Saleh, A.A., Abd El-Aziz, R.M. and El-Magd, M.A. (2021). Association of GH
polymorphisms with growth traits in buffaloes. Domestic Animal Endocrinology,
74:106541.https://doi.org/10.1016/j.domaniend.2020.106541
Jia, J.L., Zhang, L.P., Wu, J.P., Ha, Z.J. and Li, W.W. (2014). Study of the correlation between
GH gene polymorphism and growth traits in sheep. Genetic and Molecular Resource,
13:7190–7200.
Mulgai, A. W.T. and Hanotte, O. (2013). The origin of African sheep: Archaeological and genetic
perspectives. African Archeological Review, 30:39-50.
Ovimaps, (2023). Ovilocation map: Ovi earth imagery. Accessed: 23rd October, 2023.
Reaume, C.J. and Sokolowski, M.B. (2011). Conservation of gene function in behavior.
Philosophical Transactions of the Royal Society B: Biological Sciences, 366: 2100
-2110. doi:10.1098/rstb.2011.0028.
Supakorn, C. (2009). The Important Candidate Genes in Goats - A Review. Walailak Journal of
Science and Technology, 6(1): 17-36. http://dx.doi.org/10.2004/wjst.v6i1.70
Tamura, K., Peterson, D. and Peterson, N. (2011). MEGA5: Molecular evolutionary genetics
analysis using maximum likelihood, evolutionary distance, and maximum parsimony
methods. Molecular Biology and Evolution, 28(10): 2731-9.

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