International Journal of Agriculture and Earth Science (IJAES )

E- ISSN 2489-0081
P- ISSN 2695-1894
VOL. 11 NO. 1 2025
DOI: 10.56201/ijaes.vol.11.no1.2025.pg1.17


Histological Changes in the Gills and Liver of African Catfish ( Clarias gariepinus) Juveniles Exposed to Acute Concentrations of Aqueous Crude Fruit Extract of Gum Arabic Tree ( Acacia nilotica )

Damshit, M. J., Audu, B.S. And Wade, J. W.


Abstract


Histological alterations are used as indicators of aquatic pollutants and overall health of the entire population of organisms in an ecosystem. These histological parameters are related to other biomarker of stress since many pollutants have to undergo metabolic activation in order to be able to provoke cellular changes in the affected organism. Apparent healthy One hundred and twenty (120) juvenile of Clarias gariepinus, mean weight 9.77 ± 0.42g and mean length of 13.8 ± 0.80cm were used. Fish were placed in twelve (12) circular plastic tanks (40 × 30 × 20cm) and ten (10) fish were placed in each of the tanks and were allowed to acclimatize tolaboratory conditions for a period of 2 weeks during which the fish were fed with Vital feed® once daily at 3% of their body weight. The gills and liver were excised and processed, by using routine histological screening. Histological section through the gills and liver of C. gariepinus juveniles exposed to acute concentrations of aqueous crude fruit extract ofAcacia nilotica revealed some severe histological alterations such as complete loss of secondary lamellae, massive presence of inflammatory cells, and degeneration of connective tissue, hypertrophy and hyperplasia which increased as the toxicant concentration increased. The acute bioassay of aqueous crude fruit extract of A. nilotica on C. gariepinus juveniles revealed that the substance is toxic to the exposed fish at various concentrations and the result showed the 96hrLC50 caused 50% mortality. The toxicant had adverse effects on the water qualities, behavioral signs & histological parameters of the exposed fish compared to the control groups. The disruption in these parameters could have led to the degrees of impairment in the gills and livers of the exposed fish and the inability of the exposed fish to withstand infections, thereby causing a great concern for fish survival. Therefore, the use of Acacia niloticain wate


keywords:

Histological, Gills, Liver, Clarias gariepinus, Acute, Acacia nilotica


References:


Agrawal, A., Panday, R. S., & Sharma, B. (2010). Water pollution with special reference to
Pesticide contamination in India. Journal of Water Resources and Protection, 2(5), 432-
Altinok, I. &Capkin, E. (2007). Histopathology of rainbow trout exposed to sublethal

concentrations of methiocarb, a carbamate pesticide, to rainbow trout. Toxicologic
Pathology, 35(3), 405-410.
Al-Zaidan, A. S. (2017). The acute effects of un-ionized ammonia on Zebrafish (Danio rerio).
Fisheries and Aquaculture Journal, 8(3), 2-10.

APHA (American Public Health Association) (1985). Standard Methods of Examination of Water

and Waste water 15th Edition Washington D.C, Pp. 413-426.

Audu, B.S., Damshit, M. Omirinde, J.O., Wakawa, I.A,, Suleiman, Y., &Wade, J. W., (2020).

Histopathological Alterations in the morphology of the gills and liver of Clarias

gariepinusFingerlings exposed to Acute concentrations of Waste Dry Cell Battery.

Sokoto Journal of Veterinary Sciences, 19(1),26 - 33.

Audu, B. S., Adamu, K. M., &Nonyelu, O. N. (2014). Changes in haematological parameters of

Clarias gariepinus exposed to century plant (Agave americana) Leaf dust.
International Journal Applied Biological Research, 6(1), 54-65.
Audu, B. S., Omirinde, J. O., Gosomji, I. J., &Wazhi, P. E. (2017). Histopathological changes in
the gill and liver of Clarias gariepinus exposed to acute concentrations of Vernonia
amygdalina. Animal Research International, 14(1), 2576-2587.
Ayotunde, E. O., Offem, B. O. &Bekeh, A. F. (2011). Toxicity of Carica papaya Linn:
Haematological and piscidal effect on adult catfish (Clarias gariepinus). Journal of
Fisheries and Aquatic Science, 6(3), 291-308.
Capkin, E., Altinok, I. & Karahan, S. (2006). Water quality and fish size affect the toxicity of the
Endosulfan, an organochloride pesticide to Rainbow trout, Chemophere, 64 (10), 1793-
De la Torre, F., Ferrari, L., &Salibian, A. (2005). Biomarkers of a native fish species
(Cnesterodondecemmaculatus) Application to the water toxicity assessment of peri-urban
polluted river of Argentina. Chemosphere, 59(4), 577-583.



Di Giulio, R. T., & Hinton, D. E. (2008). The toxicology of fishes. Taylor & Francis Group: CRC
Press USA 1101.1st ed, pp.1096.
Drury, R., & Wallington, E. (1980). Histological technique 4th edition, Oxford University Press,
USA, pp. 272-280.
Fanta, E., Rios, F., Romao, S., Vianna A. &Freisberger. S. (2003).Histopathology of the fish
Corydoras paleatus contaminated with sublethal levels of organophosphorus in water and
food. Ecotoxicology and Environmental Safety, 54(2), 119-130.
Fatima, A. S. & Mohamed. (2009). Histopathological studies on Tilapia zilli and Solea
vulgarisfrom lake Qarum,Egypt. World Journal of Fish and Marine. Sciences, 1(1), 29-39.
Hadi,
A. A., & Alwan, S. F. (2012). Histopathological Changes in Gills, Liver and Kidney of Fresh
Water Fish, Tilapia zilli, Exposed to Aluminum. International Journal of Pharmacy & Life
Sciences, 3(11), 2071-2081.
Malcolm. J. (1994). Fish Biogenetics Chapman and Hall fish and Fisheries Series, Chapman and
Hall, London Series. Springer Nature, 5(3), 389 – 390.

Maria, V. L., Ahmed, L., Olivera, M., Serafim, A., Bebiano, M. J., Pacheco, M. & Santos, M. A.
(2009). Liver antioxidant and damage responses at Aveiro lagoon in wild juvenile
Dicentrachus Labrax L. Ecotoxicology and Environmental Safety, 72(1), 1861-1870.
Patin, S. (2004). Environmental Impact of the offshore oil and gas industry. 1stedition, United
State, pp737-748.
UNEP (United Nations Environment Programme) (1989). Estimation of the lethal toxicity of
pollutants in marine fish and vertebtates. Reference Methods for Pollution, No.43, Ville-
frenche-Surmer, France, pp.1-27.
Van der Oost R., Beyer, J., & Vermeulen, N. P. E. (2003). Fish bioacculation and bio marker in
Environmental risk assessment: A Review. Environmental Toxicology and Pharmacology,
13(2), 57-149.
Vinodhini, R., & Narayana, M. (2009). The impact of toxic heavy metals on the hematological
parameters in Common carp (Cyprinus Carpio L.). Journal of Environmental Health
Science & Engineering, 6(1), 23-28.



Wade, J. W.,Omoregie, E. &Ezenwaka, I. (2002). Toxicity of Cassava (Manihot esculenta Crantz)

effluent on the Nile Tilapia, Oreochromis niloticus(L) under laboratory conditions.

Journal of Aquatic Sciences, 17 (2): 89 - 94.
Zdenka, S., Richard, L., Jana, M., & Blanka, V. (1993). Warer quality and fish health. ISBN 92-
5-103437-0. http://www.fao.org/3/t1623E03.htm, access 14th/09/2018.


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