IIARD International Institute of Academic Research and Development

JOURNAL OF BIOLOGY AND GENETIC RESEARCH (JBGR )

E-ISSN 2545-5710
P-ISSN 2695-222X
VOL. 11 NO. 2 2025
DOI: 10.56201/jbgr.vol.11.no2.2025.pg74.85

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Study of Genetic Variability, Proximate Analysis and DNA Yield in Diverse Tomato Genotypes, Lycopersicon Esculentum (Mill.)

Adewusi, O F

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Abstract

This study was conducted on the “Study of genetic variability, proximate analysis and DNA yield in Tomato, Lycopersicon esculentum (Mill.) Genotypes”. The study aimed to determine the to determine the extent of variation, nutritional composition and DNA sample yield among the tomato genotypes with a view of identifying and selecting promising tomato genotypes that could be utilized for further tomato breeding program. The twelve-tomato genotypes utilized for this study were obtained from Tomato germplasm collection of National Center for Genetic Resources and Biotechnology (NACGRAB), Department of Plant Genetic resources, Ibadan, Oyo-State, Nigeria. The experiment was carried out at the Teaching and Research Farm, Federal University of Technology, Akure. The field experiment was laid out in a randomized Complete Block Design (RCBD), with two replications. Data were collected on 10 agronomic characters. The DNA extraction was carried out using Sodium dodecyl sulfate (SDS) and Zymo Kit protocols. The statistical analysis was carried out using SAS version 9.2. The concentration and yield of the DNA samples were determined using spectrophotometric approach. The analysis of variance revealed that the tomato genotypes were significantly different for all the characters except Plant height at flowering. The phenotypic coefficient of variation estimates were generally higher than the genotypic coefficient of variation estimate being maximum in total yield per plant (46.869% and 42.569%) for phenotypic coefficient of variation and genotypic coefficient of variation respectively. The heritability estimates were generally high for all the characters being maximum in Days to flowering (97.544%). The genetic advance estimates were generally high for all the characters studied. The high heritability coupled with high genetic advance estimates for days to flowering, days to maturity, plant height at maturity, number of fruits, number of clus

keywords:

Genetic Variability, Proximate Analysis, Dna Yield, Diverse, Tomato Genotypes

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