IIARD International Institute of Academic Research and Development

INTERNATIONAL JOURNAL OF CHEMISTRY AND CHEMICAL PROCESSES (IJCCP )

E-I SSN 2545-5265
P- ISSN 2695-1916
VOL. 11 NO. 2 2025
DOI: 10.56201/ijccp.vol.11.no2.2025.pg45.60

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Synthesis of Zinc Oxide Nanoparticles Using Colocasia Esculenta Leaves Extract and Antimicrobial Studies of White Yam Pathogens

Ugosor, P.T, Shausu, A.A

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Abstract

Zinc oxide nanoparticles (ZnO NPs) were synthesized by a green method using aqueous leaves extract of Colocasia esculenta, L and characterized by UV-Visible, XRD, SEM, EDX and FTIR. The data obtained from the zone of inhibition (mm) was analyzed using the statistical package for social Sciences, SPSS Version 20. Results were reported as Mean ± SD. The statistical difference between more than 2 groups of data was evaluated using ANOVA with LSD post hoc test. Differences between means were considered significant at p < 0.05. The study revealed that the maximum rate of synthesis could be achieved with 0.50 moldm-3 ZnO solution at 90 oC in 5 hours. Well segregated wurtzite hexagonal crystalline ZnO NPs with average crystallite size of 10 nm, ranging from 9.85 nm - 10.12 nm were obtained. FTIR spectra of the extract and the synthesized ZnO NPs revealed reducing agents such as phenolic groups as well as capping and stabilising agents such as amines, peptides and amides groups. The biosynthesized ZnO NPs exhibited antimicrobial action in a dose-dependent manner against five white yam pathogenic fungi: Aspergillus niger, Aspergillus flavus, Botryodioplodia theobromae, Phizopus stolenifera and Fusarium oxysporum as well as three bacteria: Klebsiella oxytoca, Serratia marcenscens and Pseudomonas aeruginosa. The biosynthesized ZnO NPs exhibited effective to moderately effective inhibition ranging from 89.95 % to 32.22 % on the test organisms. The ZnO NPs favourably with standard antifungal (Ketoconazole) and antibacterial (Septrim) agents. The ZnO NPs holds great potential in reducing postharvest white yam tuber rot and many other related agricultural products losses as well as a source of active ingredients for antimicrobial drug formulation and development.

keywords:

Phytochemicals, nanotechnology, biosynthesis, pathogens, antimicrobial activity.

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