IIARD International Institute of Academic Research and Development

INTERNATIONAL JOURNAL OF HEALTH AND PHARMACEUTICAL RESEARCH (IJHPR )

E-ISSN 2545-5737
P-ISSN 2695-2165
VOL. 10 NO. 5 2025
DOI: 10.56201/ijhpr.vol.10.no5.2025.pg91.100

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Capase-3 Expression Remain Unchanged in Trichloroacetic Acid and Clove Isolates Treated Adult Rats

Irozuoke A.C.

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Abstract

Apoptosis, a critical biological process, relies on caspase-3 as a key mediator in regulating cell populations and maintaining tissue homeostasis. Dysregulation of caspase-3 expression is implicated in various pathological conditions. Trichloroacetic acid (TCA) and clove isolates, known for diverse biological activities, have unclear effects on caspase-3 expression. This study investigates the impact of TCA and clove isolates on caspase-3 expression in adult rat tissues, aiming to evaluate their safety and potential therapeutic applications. Sixty adult male Wistar rats were divided into ten groups and administered various substances for specified durations. Negative controls received normal saline, while other groups received TCA, clove isolates (EIC), or a combination via orogastric canula. Caspase-3 expression was assessed in various tissues. Caspase-3 immunostaining revealed very good immunoreactivity across groups, with reduced staining in groups 4 to 10. Morphological scores showed variation in immunoreactivity. Photomicrographs illustrated immuno-positive markers for Caspase-3 in testes samples from each group. The study demonstrates that Caspase-3 expression remains unchanged in response to Trichloroacetic Acid and Clove Isolates treatment in adult rats up to a certain point. Reduced immunoreactivity in groups 4 to 10 necessitates further investigation into molecular mechanisms and potential therapeutic implications. Future research should identify specific components responsible for observed effects, enhancing targeted understanding of Trichloroacetic Acid and Clove Isolates impact on testicular apoptosis.

keywords:

Apoptosis, Caspase-3 expression, Trichloroacetic Acid (TCA), Clove Isolates, Adult rats, Immunostaining, Morphological scores.

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