International Journal of Agriculture and Earth Science (IJAES )

E- ISSN 2489-0081
P- ISSN 2695-1894
VOL. 11 NO. 4 2025
DOI: 10.56201/ijaes.vol.11.no4.2025.pg29.41


Bio-stimulatory Potential of Intestinal Waste of Cow on a Crude Oil Polluted Soil

Oriakpono, Iris O, Wemejiri Anwuri, Ukaulor, Victor C, and Oriakpono, Obemeata, E


Abstract


The study was carried out to examine the biostimulatory effect of intestinal wastes of cows on the bioremediation on a crude oil contaminated soil. A completely randomized design was applied with four rates (0, 1, 2 and 7Kg) of intestinal waste of cow and (0, 1, 2 and 5L) of crude oil were applied to contaminate the soil per plot. The soil physico-chemical parameters analysed includes pH, Total Organic Content (TOC), Nitrogen (N), Carbon to Nitrogen ratio (C:N), Phosphorus (P), cation exchange capacity (CEC), total petroleum hydrocarbon (TPH) and poly aromatic hydrocarbon (PAH). The result for soil physio-chemical parameters revealed that the mean value of soil nitrogen, C:N, TOC, TPH and PAH increased in the 2 weeks after pollution while the level reduced significantly (p< 0.05) at 4 weeks after bioremediation. The mean value of CEC, soil conductivity and soil phosphorus decreased in the 2 weeks after pollution when compared to the pre-exposed soil while the level increased significantly (p< 0.05) at 4 weeks after bioremediation. This research has proven that using intestinal waste of cow which is an animal waste that is generated in abattoirs has a strong bio-stimulatory properties and is effective in bio-remediation, therefore should be adopted in the restoration of crude oil polluted soil.


keywords:

Biostimulation, Intestinal Waste, Cow waste, Crude oil and Polluted soil


References:


Adams, G.O., Fufeyin, P.T., Okoro, S.E. and Ehinomen, I. (2015). Bioremediation,
Biostimulation and Bioaugumentation: A review / international Journal of
Environmental Bioremediation and Biodegradation ,3(1): 28-39.
Adams, P.Z., Jackson, P. P. (1996). Bioremediation of oil spill. Theory and Practice. In Nigeria
National Petroleum Corporation Publication. Department of Petroleum Resources. 3-
Adeniyi, E. O., Oluwole, B. A., Amyaye, G. K. (1983). Environmental and social economic
impacts of oil spillage in the petroleum producing service area of Nigeria.
Environmental Impact Statement and Assessment.8, 233-237.
Agarry, S.E., Aremu, M.O. and Aworanti, O.A. (2013). Kinetic modelling and half- life study
on enhanced soil bioremediation of bonny- light crude oil amended with crop and
animal derived organic waste. Petroleum and Environmental Biotechnology.4:137-14.
Atlas, R. M. (1981). Biodegradation of Petroleum Hydrocarbon: An Environmental
Perspective Microbiological Review 45,180-209.
Basumatary B, Saikia R, Bordoloi S. (2012). Phytoremediation of crude oil contaminated soil
using nut grass, cyperusrotundus. Journal of Environmental Biololgy.;33(5):891-896
Beckles, M. D., Ward, C. H., Hughes, J. E., (1998). Effect of mixtures of polycyclic aromatic
hydrocarbons and sediments of fluoromethane biodegradation pattern Environ.
Toxicol. Chem., 17, 1246-1257.
Brady C N, and Weil RR (2002). Nature and properties of soils, 2002,13th Ed. Prentice Hall
Chikere, C.B., Okoye, A.U. and Okpokwasili, G.C. (2016). Microbial community profiling of
active oleophilic bacteria involved in bioreactor based crude oil polluted sediment
treatment. Journal of Applied and Environmental microbiology. 4(1): 1-20.
Cornell University Cooperative Extension (CUCE) (2007) Cation Exchange Capacity (CEC).
Agronomy Fact Sheet Series # 22. Department of Crop and Soil Sciences, College of
Agriculture and Life Sciences, Cornell University
Dutta, T. K. and Harayama, S. (2001). Analysis of long side chain alkyl aromatics in crude oil
for evaluation of their fate in the environment.Environ.Sci. Technol. 35, 102-107.
Ekperusi, O. A. and Aigbodion, I. F. (2015). Bioremediation of heavy metals and petroleum
hydrocarbons in diesel contaminated soil with the earthworm: Eudrilus eugeniae,
Springer Plus, 4: 540.
Ekpo, M. A., and Udofia, U. S. (2008). Rate of Biodegradation of Crude Oil by
Microorganisms isolated from Oil Sludge Environment. Afr. J. Biotechnol. 7, 4495-
Elektorowicz M (1994) Bioremediation of petroleum-contaminated clayey soil with
pretreatment. Environ Technol15: 373-380.
FAO, (1976). A Framwork for Land Evaluation, FAO Bulletin 32, FAO/UNESCO, France
Frank, A.O., Abiye, A.I. and Philip, O.O. (2013). Bioremediation of petroleum hydrocarbon
polluted mangrove swamps using nutrient formular produced from water Hyacint
(Eicchornia crassipes). American Jour. of Environmental sciences. 9(4):348-366
Gerhart, P. Murray., R. R. E., Kneg, N. R., Phillips, C. B. (1981). Manual of Methods of
General Bacteriology.American Society for Microbiology, Washington D.C. W. A.
Holland MD, Allen RKG, Barten D, and Murphy ST. (1989). Land Evaluation and Agricultural
for Cross River National Park, Oban Division. Prepared by the Overseas Development
National Resources Institute in Collaboration with WWF for the Federal Republic of
and the Cross River State Government.;1189
Isirimaru, N. O., Zoufa, K.,Loganathan, P. (1989). Effect of Crude oil on maize performance
and soil chemical properties in humid forest zone of Nigeria”. Discovery and
Innovation. 1, 95-98.
Jobson, A. M., Mclaughlin, M., Cook, F. D.,Westlake, D.W.S. (1974). Effects of amendment
on microbial utilization of oil applied to soil, Appl. Microbiol. 27, 166-171
Keeney, D. R., and Nelson, D. W. (1994). Determination of numeral (inorganic) Nitrogen. In:
Miller, A. L, and Keeney, D. R. (Ed.). Methods of Soil Analysis. Animal Science
Publishers. Madison. 643
Kumar S, Iyer A, and Agarwal S, (2011). Cotton Yield In Relation To Physicochemical
Properties Of Cultivated Soil Of Rajkot Region.Int.J.of Adv.Engi.Tech.,2(3),52-55.
Marshal. T. R. (1988). Biodegradation of Petroleum wastes in soil. The Microbial Ecosystem
and the Optimization of a Treatment Process.DoctoralThesis.University of Southern
California, California, USA.
Mentzer, E. and Ebere D. (1996). Remediation of hydrocarbon of hydrocarbon contaminated
sites. Proceeding industry and the Nizena Enviornment, Nigerian National Petroleum
(Corporation NNPC) 1:5-59
Nduka, J.K., Umeh, L., Okerulu, I.O., Umedum, L.N. and Okoye, H.N. (2012). Utilization of
different microbes in bioremediation of hydrocarbon contaminated soils stimulated
with organic and inorganic fertilizers. Journal of petroleum Environmental
Biotechnology. 3:116-125.
Njoku KL, Akinola MO, Oboh BO. (2009). Phytoremediation of crude oil contaminated soil:
The effect of growth of Glycine max on the physico-chemistry and crude oil content
of soil. Nature & Science.;7(10):1-2
Nnadi P. C and Osakwe J. A. (2017). Bioremediation of Crude Oil Contamination Soil with
Livestock Waste.
Okunwaye, I., Ogboghodo, I., Ewansiha, S. and Oriakpono, O. (2018). Biostimulation
Potentials of Cow Dung on a Crude Oil Polluted Soil. International Journal of Plant &
Soil Science 27(1): 1-11.
Oriakpono,O.E., Okunwaye, I. and Imafidor, O.H. (2018).A screen house experiment to
evaluate the biostimulation potentials of cow dung on a crude oil polluted soil
.International Journal of Agriculture innovation and research vol. 6(5): 2319-1473.
Perfumo, A., Banat, I. M., Marchant, R. and Vezzulli, L., (2007). Thermally enhanced
approaches
for
bioremediation
of
hydrocarbon-contaminated
soils.
Chemosphere66(1): 179-184
Piehler, M.F., Swistak, J.G., Pinckney, J.L. and Paerl, H.W. (1999) Stimulation of Diesel Fuel
Biodegradation by Indigenous Nitrogen Fixing Bacterial Consortia. Microb Ecol38:
69-78
Reisinger, H. J. (1995). Hydrocarbon bioremediation an overview. In: Hinchee. R. E., Kittel,
J. A. and Reisinger, H. J.(Eds.). Applied Bioremediation of Petroleum Hydrocarbons.
Battelle Press, Columbus. 7.
Rhykerd RL, Crews B, McInnes KJ, Weaver RW (1999) Impact of bulking agents, forced
aeration and tillage on remediation of oil-contaminated soil.Bioresour Technol 67:
279-285.
Romantschuk, M., Sarand, I., Petanen, T., Peltola, R., Jonssonvihanne, M., and Koivula, T.
(2000). Means to improve the effect of in situ bioremediation of contaminated soil: an
overview of novel approaches. Environ. Pollut. 107, 179-185
Shahunthala D. Ramachandran, P. V. Hodson, C. W. and Khan, K. L. (2004). Oil dispersant
increases PAH uptake by fish exposed to crude oil Ecotoxicology and Environmental
Safety.;59:300-308.
SVMS (2001). SPDC people and environment annual report. Shell virtual media services
(SVMS) of the shell petroleum development of Nigeria of Nigeria, pp.36-37
Tales, K. and Ingole, S. (2015). A Review on Role of Physico-Chemical Properties in Soil
Quality. Chemical Science Review and Letters, 4(13), 57 – 66
Ugochukwu C. O., Michael U. O., Kingsley C.A., Nsikak S. A., Benjamin C. O., Onyedika I.
O., and Nnenna C.N. O, (2016) “Bioremediation of Crude Oil-polluted Soil Using
BroilerChicken Droppings.” Journal of Applied & Environmental Microbiology, vol.
4, no. 4: 75-84. doi: 10.12691/jaem-4-4-2.
Walkey A, and Black, C. A. (1934). An examination of the micro?kjeldahl method of
determining soil organic matter and a proposed modification of chromic acid titration
method. Soil Science.;37:29-38
Zhu, X.; Venosa, A.D.; Suidan, M.T. and Lee, K. (2001) Guidelines for the Bioremediation of
Marine Shorelines and Freshwater Wetlands; U.S. Environmental Protection Agency,
Cincinnati, OH.


DOWNLOAD PDF

Back


Google Scholar logo
Crossref logo
ResearchGate logo
Open Access logo
Google logo