IIARD International Institute of Academic Research and Development

JOURNAL OF BIOLOGY AND GENETIC RESEARCH (JBGR )

E-ISSN 2545-5710
P-ISSN 2695-222X
VOL. 10 NO. 3 2024
DOI: 10.56201/jbgr.v10.no3.2024.pg26.36

---

Genetic Variability, Character Association and Phytochemical Analysis in Okra Genotypes, Abelmoschus Esculentus

L Moench, Adewusi OF and Omikunle O A

---

Abstract

This study was coducted on the” Genetic variability, character association and phytochemical analysis in okra genotypes, Abelmoschus esculentus (L.) Moench”. The study aimed to determine the extent of variability among the okra genotypes using phenotypic variation, correlation analysis and phytochemical analysis with a view of identifying the outstanding okra genotypes that could be utilized in future okra breeding program. The 20 okra genotypes used for this study were obtained from the okra germplasm bank of National Center for Genetic Resources and Biotechnology (NACGRAB), Ibadan, Oyo State, Nigeria. The experiment was carried out at the Teaching and Research Farm, Federal University of Technology, Akure. The experiment was laid out in a randomized complete block design with three replications. Data were collected on eleven agronomic characters. The statistical analysis was carried out using SAS version 9.2. The analysis of variance revealed that the okra varieties recorded significant differences for characters considered at 1% and 5% probability. The analysis of variance showed significant differences among the varieties for number of fruits, total fruit weight and individual fruit weight, indicating the presence of genetic variability for these characters. The phenotypic variance and phenotypic coefficient of variation were higher than the genotypic variance and genotypic coefficient of variation. The correlation analysis showed significant positive associations between fruit yield and plant height (0.772), number of fruits (0.843), number of branches (0.619), fruit weight (0.483) and fruit length (0.384) indicating that they are potential characters to target and select for in okra breeding programs. Phytochemical analysis also showed differences in phytate (21.01- 32.96mg/g), tannin (0.03-0.08mg/g), saponin (5.80-10.20mg/g), oxalate (1.80-3.69mg/g), phenols (0.01 – 0.46mg/g) and flavonoids (9.90 -11.30mg/g). It can be

keywords:

Genetic Variability, Correlation, Phytochemical, Phenotypic, Genotypic

---

References:

Ajao, A. T., Olufemi, B. and Akinmoladun, O. F. (2020). Genetic variability and heritability in
okra (Abelmoschus esculentus L.) for yield and quality traits. Scientific Reports, 11(1),
Akotkar, P. K., More, A. D. and Kakde, S. S. (2020). Genetic diversity studies in okra
(Abelmoschus esculentus L.). Journal of Applied and Natural Science, 12(2), 171–177.
Axe, J. (2021). Okra Nutrition: Improve Heart Health, Eyesight & Cholesterol Levels.
https://draxe.com/nutrition/okra-nutrition/
Boham, B.A. and Kocipai-Abyazan, R. (1974). Flavonoids and Condensed Tannins from Leaves
of Hawaiian Vaccinium vaticulatum and V. calycinum. Pacific Science, 48(4), 458-463.
Day, R.A. and Underwood, A.L. (1986). Quantitative Analysis (5th ed.). Prentice Hall.
Durazzo, A.; Lucarini, M.; Novellino, E.; Souto, E.B.; Daliu, P. and Santini, A. (2018).
Abelmoschus esculentus (L.): Bioactive Components’ Beneficial Properties-Focused on
Antidiabetic Role-For Sustainable Health Applications. Molecules 2018, 24, 38.
Ejikeme, C.M., Ezeonu, C.S. and Eboatu, A.N. (2014). Determination of Physical and
Phytochemical Constituents of Some Tropical Timbers Indigenous to Niger Delta Area of
Nigeria. European Scientific Journal, 10(18), 247-270.
Hassan, L. G. and Mahfouz, S. A. (2016). Phytochemical and antioxidant activity of okra
(Abelmoschus esculentus) varieties. Asian Journal of Plant Science & Research, 6(1), 38-
Kumar, S., Verma, S. and Saxena, S. K. (2018). Character association and path analysis in okra.
Indian Journal of Horticulture, 75(1), 98-103.
Makkar, H.P.S., and Goodchild, A.V. (1996). Quantification of Tannins: A Laboratory
Manual. International
Center for Agricultural Research in the
Dry
Areas
(ICARDA), Aleppo, Syria.
Makkar, H.P.S., Blümmel, M., Borowy, N.K. and Becker, K. (1993). Gravimetric determination
of tannins and their correlations with chemical and protein precipitation methods. Journal
of the Science of Food and Agriculture, 61(2), 161–165.
Melaku, S., Teshome, A. and Degu, A. (2020). Genetic variability, heritability, and genetic
advance in okra (Abelmoschus esculentus L.) for growth and yield traits. Ethiopian Journal
of Agricultural Sciences, 30(2), 215-223.
Melaku, S., Teshome, A. and Degu, A. (2020). Genetic variability, heritability, and genetic advance
in okra (Abelmoschus esculentus L.) for growth and yield traits. Ethiopian Journal of
Agricultural Sciences, 30(2), 215-223.
Mishra, G. P., Seth, T., Karmakar, P., Sanwal, S. K., Sagar, V., Priti, and Singh, B. (2021). Breeding
strategies for yield gains in Okra (Abelmoschus esculentus L.). In Advances in Plant
Breeding Strategies: Vegetable Crops: Volume 9: Fruits and Young Shoots (pp. 205-233).
Cham: Springer International
Publishing.
Obadoni, B.O. and Ochuko, P.O. (2002). Phytochemical studies and comparative efficacy of the
crude extracts of some haemostatic plants in Edo and Delta States of Nigeria. Global
Journal of Pure and Applied Sciences, 8(2), 203-208.
Ogunbor, G. 2020. Okra: origin, classification, uses, Pest, Disease.https://itsmyschoollibrary.com.
Okra-origin-classification-uses-pest-diease
Olayanju, I. O., Agboola, A. O. and Oladipo, O. O. (2021). Phytochemical content and antioxidant
activity of okra (Abelmoschus esculentus L.) genotypes. Journal of Food Science and
Technology, 58(1), 185-192.
Rambabu, M. and Prakash, P. (2019). Genetic variability, heritability, and association analysis for
yield and yield contributing characters in okra (Abelmoschus esculentus L. Moench).
International Journal of Current Microbiology and Applied Sciences, 8(4), 764–774.
Ranpise, S. R., Pande, M. S. and Raut, S. R. (2018). Genetic variability, heritability, and genetic
advance for yield and its components in okra. Journal of Applied and Natural Science,
10(2), 581-585.
Ravindran, V. and Samman, S. (2002). The health benefits of legumes and their phytochemicals.
Phytochemistry Reviews, 1(1), 1–10.
Samiksha, Verma, R. S., Patel, V. K., Prakash, S., Maurya, S. K., and Kumar, S. (2021). Studies
on Correlation and Path Coefficient Analysis in Okra [Abelmoschus esculentus (L.)
Moench]. International Journal of Current Microbiology and Applied Sciences, 10(3), 277-
Sofowora, A. (1993). Medicinal Plants and Traditional Medicine in Africa (2nd ed.). Spectrum
Books Ltd., Ibadan, Nigeria.
Young, S.M. and Greaves, J.E. (1940). Influence of variety and treatment on phytic acid content
of wheat. Food Research, 5, 103-105.

DOWNLOAD PDF

---

Back