IIARD International Institute of Academic Research and Development

RESEARCH JOURNAL OF PURE SCIENCE AND TECHNOLOGY (RJPST )

E-ISSN 2579-0536
P-ISSN 2695-2696
VOL. 8 NO. 1 2025
DOI: 10.56201/rjpst.vol.8.no1.2025.pg65.75

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Isolation and Identification of Bacteria Isolates Associated with Orthopaedic Wound Infections in Kano, Nigeria

Garba M, Mukhtar M.D, Yusha’u M, Aminu M. B and Mohammad, A.B

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Abstract

Orthopedic wound infections are global problems in the field of orthopedic and hospital in general. The aim of this study was to isolate and identify bacteria in orthopedic wounds infection of patients attending selected hospitals in Kano, Northern Nigeria. A total of 400 samples were collected from 5 tertiary health institutions in Kano state (National Orthopedic Hospital, Dala, Aminu Kano Teaching Hospital, Sir Muhammad Sunusi Specialist Hospital, Sheikh Jidda Specialist Hospital and Murtala Muhammad Specialist Hospital, Kano). A sample of exudates from each wound site was carefully taken using sterile swab stick under aseptic procedures, inoculated onto culture media and incubated at 37oC for 24 hours period of incubation. Identification of bacterial isolates was done using macroscopic, culture, microscopy and biochemical test according to standard microbiological technique. Antibiotic susceptibility testing was performed using disc diffusion method. The result of the study showed that out of 400 pus samples collected from orthopedic patient 336 (84%) samples yielded growth and 64 (16.0%) samples had no growth. In the study, 443 bacteria were isolated, Gram negative bacteria were predominant with 258 (58.2%) isolates, while Gram positive bacteria contributed 185 (41.8%) of total isolates. Altogether 13 different bacterial species were isolated, among which Staphylococcus aureus 139 (27.1%) were predominant followed by Pseudomonas aeruginosa 123 (45.0%), and the lowest were S. pyogenes 4 (1.2%).

keywords:

Bacteria, identification, isolation, Kano, orthopedic wound

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