IIARD International Institute of Academic Research and Development

International Journal of Agriculture and Earth Science (IJAES )

E- ISSN 2489-0081
P- ISSN 2695-1894
VOL. 10 NO. 10 2024
DOI: 10.56201/ijaes.v10.no10.2024.pg191.202

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Evaluation of Residual Total Petroleum Hydrocarbon (TPH) in Rhizosphere of Garden Egg ( Solanum melongena ) and Non rhizosphere Soil Contaminated with Crude Oil Under Different Organic Manure Treatments

Ordu, J.O. & Disegua, G.C.

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Abstract

This study was undertaken to evaluate residual Total petroleum hydrocarbon (TPH) in rhizosphere of Solanium melongena (Garden Egg) and non-rhizosphere soils contaminated with crude oil under different organic manure treatments, with the rhizosphere microbial community acting as plant-microbe interaction at subsurface environment. Enumeration of total heterotrophic bacteria and total saprophytic fungi were carried out using plant rhizosphere soil samples. The rhizosphere soil samples were collected from crude oil contaminated soil amended with organic nutrients: poultry droppings, goat faeces, cow dung and fish pond waste water i.e. water from pond containing Clariesgariepenus mixed with its faeces and feeds. The plant rhizosphere sample was Solanium melongena (Garden Egg). A non-rhizosphere soil was used as control. The representative strains or genera of both bacteria and fungi were identified. Total heterotrophic bacterial count range from 3.5x10 to 8.21x106 colony forming units per log10 CFU/g log 10 of sample while total fungal counts ranged from 4.30x105 to 8.67x106 log 10 CFU/g; Non-rhizosphere soil (control) samples, had colony counts within the range of 4.30x103 to 4.71x105 for both bacteria and fungi; the rhizosphere were also screened quantitatively for the presence of hydrocarbon utilizing fungi (HUF) and hydrocarbon utilizing bacteria (HUB) and to evaluate the rate of degradation of crude oil by pure cultures over a period of 28 days in mineral salt broth with crude oil as sole source of carbon. While an experimental set-up mineral salt broth with crude oil without any inoculums served as control. The analysis of pH and total viable counts were carried out at weekly intervals while total petroleum hydrocarbon content was determined at bi weekly interval. Results showed percentages of crude oil degradation in the control and crude oil polluted bioremediated rhizosphere soils, which percentages of crude oil deg

keywords:

crude oil, Solanium melongena (Garden Egg), rhizosphere soil, Total Petroleum

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