IIARD International Institute of Academic Research and Development

International Journal of Agriculture and Earth Science (IJAES )

E- ISSN 2489-0081
P- ISSN 2695-1894
VOL. 10 NO. 10 2024
DOI: 10.56201/ijaes.v10.no10.2024.pg14.26

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Acute Toxicity and Active Ingredients Concentration of Datura innoxia Stem on Clarias gariepinus Juveniles

Iorhiin, R.M., Odum, C. J. , Isiyaku, M. S., & Olamide, A.S.

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Abstract

Despite the widespread use of Datura innoxia stem in Nigeria for demarcation, gardening and medicine, its toxicity and effectiveness to aquatic species, notably fish, has received little attention. This study was carried out to determine the toxicity, phytochemical and proximate composition of Datura innoxia stem to Clarias gariepinus. The range-finding test was carried out and repeated twice to obtain credible results that could be used in the ultimate test .The toxicant (Datura_ innoxia stem) was introduced at varying concentrations of 300mg, 330mg, 360mg, 390mg and 420mg, respectively in the initial range-finding test; nevertheless, the toxicant was added at different concentrations of 320mg, 330mg, 340mg, 350mg, and 360 milligrams per liter of water (mg/l) in the final test. The median lethal concentration of Datura innoxia stem powder to Clarias gariepinus was determined to be 334.370 mg/L based on the conclusive test. The study found that young Clarias gariepinus exhibited erratic movement at first, rapid opercular movement, skin darkening, and absence of response. Furthermore, the results show that the local composition was within acceptable limits. Among the phytochemicals discovered were steroids, tannins, saponins, phenols, cardiac glycosides, and scopolamine. The water quality data were evaluated using one way Analysis of Variance (ANOVA) with temperature range of 25.27 to 25.350C, As a result, the study suggests that Datura innoxia stem is hazardous to Clarias gariepinus juveniles.

keywords:

Datura_innoxia stem, Acute Toxicity, Lethal Concentration, and Active Ingredients

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