IIARD International Institute of Academic Research and Development

INTERNATIONAL JOURNAL OF HEALTH AND PHARMACEUTICAL RESEARCH (IJHPR )

E-ISSN 2545-5737
P-ISSN 2695-2165
VOL. 9 NO. 5 2024
DOI: 10.56201/ijhpr.v9.no5.2024.pg61.72

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Antibiotic resistant Uropathogenic Klebsiella spp. and

E coli isolates and their Multidrug resistance pattern in three major Hospitals in Kano State Ishaq SA , Yusha’u M , Gambo S , Mohammed N , Abdullahi ZH :

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Abstract

Many bacterial species have been reported to develop resistance to antibiotics commonly prescribed for urinary tract infections. Therefore, the need to search for natural alternative for remedy of this problem cannot be overemphasized. One hundred and thirty-one (131) suspected E. coli and Klebsiella sp. were collected from patients with suspected urinary tract infection from the three major hospitals in Kano State. Purification and identification of the bacterial isolates were carried out using standard microbiological methods. Basic biochemical tests and MicroGen identification kit were used identification of the isolates. The antibiotic susceptibilities of the E coli and Klebsiella sp. was determined using the modified Kirby-Bauer diffusion method and the multidrug resistant isolates, extended drug resistance as well as pandrug resistance were determined using standard microbiological methods. Multiple antibiotic resistance (MAR) index is a measure of the extent of antimicrobial agent resistance for the isolates in the group studied. It was calculated as a/b, where a represents the number of antibiotics to which the isolates were resistant and b represents the total number of antibiotics to which the isolate was exposed. Out of the 131 isolates collected from the three major hospitals, 42.8%, 24.4% and 32.8% were obtained from Murtala Muhammad specialist hospital, Aminu teaching hospital and Muhammad Abdullahi Wase teaching hospital respectively. The biochemical tests showed that 30 (30.9 %) E coli, 10 (10.3) % Klebsiella sp, 29 (29 %), other Enterobacteriaceae, 28 (28.9) % non- Enterobacteriaceae. Of the 10 Klebsiella sp, 8 (80 %) were K oxytoca while 2 (20 %) were K pneumoniae. All the Klebsiella sp. and E coli isolates (n=40) were susceptible to gentamicin (100%), 75 % susceptible to ciprofloxacin and 67% to amoxicillin-clavulanic acid. The proportion of the isolates resistant to cephalothin was 77.5%, 72.5% to cefpodoxi

keywords:

Multiple antibiotic index, E coli, Klebsiella sp, UTI.

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International Journal of Health and Pharmaceutical Research E-ISSN 2545-5737
P-ISSN 2695-2165 Vol. 9. No. 5 2024 www.iiardjournals.org Online Version





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