IIARD International Institute of Academic Research and Development

INTERNATIONAL JOURNAL OF MEDICAL EVALUATION AND PHYSICAL REPORT (IJMEPR )

E-ISSN 2579-0498
P-ISSN 2695-2181
VOL. 8 NO. 6 2024
DOI: 10.56201/ijmepr.v8.no6.2024.pg57.74

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Investigation of PAX3 and PVT polymorphisms of genes in patients with type diabetes in Iran by Tetra ARMS-PCR method

Hoda Yaser Aswad Al-Zarijawi

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Abstract

Background and hypothesis Evidence from genetic epidemiology suggests that type 2 diabetes has a strong genetic basis. The aim of this study is to study the polymorphisms of PAX3 and PVTI genes in type 2 diabetes patients in Iran Tetra ARMS-PCR Cat Method . Research Method Then 10 blood samples were collected from type 2 diabetes patients in Milad Hospital in Tehran and stored in the refrigerator. 10 control samples were considered and demographic information was collected for all subjects. DNA extraction was performed using a special kit and the quality of the extracted DNA samples was checked by agaron gel electrophoresis. T-ARMS PCR technique was used to identify the genetic polymorphisms associated with (3) rs2305619 and (2648875) PVT1) using designed primers and were placed on electrophoresis for genetic and allelic distribution, then using the program SPSS version 23 for Chi-Deva test to check the quantitative data in these two groups after verification Check the validity of the data and then use the Mann-Wedney test . The results of the examination of the repeat fragments in the present study on agarose gel showed that in (2305619) A3 (1(2648875) .Polymorphisms as well as the presence of formed bands indicate the presence of A and G alleles in both women Statistical calculations showed that the polymorphisms of the PAX3 and PVTI genes and the disease. There was no statistically significant association with type 2 diabetes in the population included in the study (0.05). The highest relative genetic frequencies were seen in the AA and GG genotypes in PVTIS PAX3, respectively, which had the greatest role in type 2 diabetes and excluded gender and body mass index, while cholesterol level, fasting blood sugar and triglycerides. HBAIC had a significant effect on type 2 diabetes and was reported as significant , m 1005. Conclusion of polymorphisms (2305619) X3 and (12648875) PVT1 in type 2 diabet

keywords:

Polymorphism Type 2 diabetes Demographics Diabetes PVTI PAX3 Tetra ARMS-PCR

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