IIARD International Institute of Academic Research and Development

INTERNATIONAL JOURNAL OF HEALTH AND PHARMACEUTICAL RESEARCH (IJHPR )

E-ISSN 2545-5737
P-ISSN 2695-2165
VOL. 9 NO. 4 2024
DOI: 10.56201/ijhpr.v9.no4.2024.pg64.70

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Effect of Justicia Carnea Leaf Extract on Plasma and Fecal Lipid Profile in High-Fat Diet Fed Wistar Rats

Onyebuchi Obia and Joy Eifuobhokhan

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Abstract

The preference for high-fat diets has increased the risk of developing cardio-metabolic metabolic disorders. The aim of this study was to examine the effect of Justicia carnea (JC) leaf extract on plasma and fecal lipid profile [total cholesterol (TC), triglyceride (TG), high density lipoprotein (HDL), low density lipoprotein (LDL)] in high-fat diet fed wistar rats. The study involved a total of thirty wistar rats separated into six groups of five rats each. Group 1 served as control while groups 2 to 6 were fed with high-fat diet (HFD) throughout the period of the experiment. Group 2 remained untreated, Groups 3, 4 and 5 received respectively 200mg/kg, 500mg/kg and 1000mg/kg of JC extract. Group 6 received 10mg/kg of simvastatin. The animals were fed with the extract for twenty-eight days and thereafter plasma and fecal samples were collected to determine the lipid profile. Daily administration of 200mg/kg of JC caused a significant reduction in the plasma levels of total cholesterol (TC) but significant increase in fecal total cholesterol (FTC). The higher doses also showed a similar pattern but not significantly. Administration of 500mg/kg of JC caused significant increase in plasma TG and HDL compared to the HFD only group. All three doses of JC caused significant reduction in LDL. Conclusively, the results from the present study suggest that JC could possess plasma lipid lowering property and also enhanced fecal excretion of cholesterol. This effect could be possibly due to decreased absorption of lipids and increased fecal excretion of lipids.

keywords:

Justicia carnea, plasma, fecal, lipid profile

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References:

1. Jeong, W. I., Jeong, D. H., Do, S. H., Kim, Y. K., Park, H. Y., Kwon, O. D., Kim, T. H., &
Jeong, K. S. (2005). Mild hepatic fibrosis in cholesterol and sodium cholate diet-fed rats. The
Journal of veterinary medical science, 67(3), 235–242.
Oggioni, C., Cena, H., Wells, J.C.K., Lara, J., Cells-Morales, C., & Siervo, M. (2015).
Association between worldwide dietary and lifestyle pattern with total cholesterol concentrations
and DALYs for infectious diseases: An Ecological Analysis. Journal of Epidemiology and
Global Health. 5 (4): 315-325.
Zong, G., Li, Y., Wanders, A. J., Alssema, M., Zock, P. L., Willett, W. C., Hu, F. B., & Sun,
Q. (2016). Intake of individual saturated fatty acids and risk of coronary heart disease in US men
and women: two prospective longitudinal cohort studies. BMJ (Clinical research ed.), 355,
i5796.
Houston, M. (2018). The relationship of saturated fats and coronary heart disease: fa(c)t or
fiction? A commentary. Therapeutic advances in cardiovascular disease, 12(2), 33–37.
Buettner, R., Parhofer, K.G., Woenckhaus, M., Wrede, C.E., Kunz-Schughart, L.A.,
Schölmerich, J., Bollheimer, L.C (2006): Defining high-fat-diet rat models: metabolic and
molecular effects of different fat types. Journal of Molecular Endocrinology. 6 (3): 485-501.
Gao, M.; Ma, Y.; Liu, D. (2015). High-Fat Diet-Induced Adiposity, Adipose Inflammation,
Hepatic Steatosis and Hyperinsulinemia in Outbred CD-1 Mice. PLOS ONE. 10, e0119784.
von Frankenberg, A. D., Marina, A., Song, X., Callahan, H. S., Kratz, M., & Utzschneider, K.
M. (2017). A high-fat, high-saturated fat diet decreases insulin sensitivity without changing
intra-abdominal fat in weight-stable overweight and obese adults. European journal of nutrition,
56(1), 431–443.
Guo, Z., Ali, Q., Abaidullah, M., Gao, Z., Diao, X., Liu, B., Wang, Z., Zhu, X., Cui, Y., Li,
D., & Shi, Y. (2022). High fat diet-induced hyperlipidemia and tissue steatosis in rabbits through
modulating ileal microbiota. Applied microbiology and biotechnology, 106(21), 7187–7207.
Bendsen, N. T., Christensen, R., Bartels, E. M., & Astrup, A. (2011). Consumption of
industrial and ruminant trans fatty acids and risk of coronary heart disease: a systematic review
and meta-analysis of cohort studies. European journal of clinical nutrition, 65(7), 773–783.
Siervo, M., Lara, J., Chowdhury, S., Ashor, A., Oggioni, C., & Mathers, J. C. (2015). Effects
of the Dietary Approach to Stop Hypertension (DASH) diet on cardiovascular risk factors: a
systematic review and meta-analysis. The British journal of nutrition, 113(1), 1–15.
Samadian, F., Dalili, N., & Jamalian, A. (2016). Lifestyle Modifications to Prevent and
Control Hypertension. Iranian journal of kidney diseases, 10(5), 237–263.
Ozemek, C., Laddu, D. R.., Arena, R., & Lavie, C. J. (2018). The role of diet for prevention
and management of hypertension. Current opinion in cardiology, 33(4), 388–393.
Ogan, P.M., Obia, O., & Apugo, U.I. (2022). Effect of hydo-ethanolic extract of Solanum
aethiopicum fruit on the lipid profile of wistar rats. Global Scientific Journals. 10 (3): 2483-
Obia, O., Ezekiel-Hart, H., Laz-Okenwa, J.O.A., Reuben, E., Dan-Jumbo, Dagbota & Wami-
Amadi, C.F. (2024): The effect of hydro-methanolic seed extract of Azanza garckeana (goron
tula) on lipid profile and oxidative stress markers of wistar rats. International Journal of Medical
Evaluation and Physical Report. 8 (5): 83-89.
Onyeabo, C., Achi, N. K., Ekeleme-Egedigwe, C. A., Ebere, C. U., & Okoro, C. K. (2017).
Haematological and biochemical studies on Justicia carnea leaves extract in phenylhydrazine
induced-anemia in albino rats. Acta scientiarum polonorum. Technologia alimentaria, 16(2),
217–230.
Nji, A. A., Chrysanthus, N., & Monyongo, N. D. (2020). Effects of Justicia carnea Leave on
Hematological Parameters in Albino Mice Carried Out in Mbingo Annex Hospital Laboratory in
Bamenda, North West Region, Cameroon. Journal of Advances in Microbiology, 20(10), 43–50.
Iwetan, B. B., Obianime, A. W., Ewhre, L. O. and Kweki, G. R. (2022) “The Antioxidant
Modulating Properties of Justicia carnea Extract on Sheep Red Blood Cells Immunized Mice”,
Journal of Pharmaceutical Research International, 34(43B), pp. 58–74.
Boqué, N., Campión, J., Iglesia, R., Garza, A.L., Milagro, F.I., San Román, B., Banuelos,
O.J., Martanez, A (2012). Screening of polyphenolic plant extracts for anti-obesity properties in
Wistar rats. Journal of Science and Food Agriculture. 93:1226-1232.
Lichtenstein A. H. (1990). Intestinal cholesterol metabolism. Annals of medicine, 22(1), 49–
Lin, X., Racette, S. B., Ma, L., Wallendorf, M., & Ostlund, R. E., Jr (2017). Ezetimibe
Increases Endogenous Cholesterol Excretion in Humans. Arteriosclerosis, thrombosis, and
vascular biology, 37(5), 990–996.
Grefhorst, A., Verkade, H. J., & Groen, A. K. (2019). The TICE Pathway: Mechanisms and
Lipid-Lowering Therapies. Methodist DeBakey cardiovascular journal, 15(1), 70–76.
Chen, L., Zhao, Z. W., Zeng, P. H., Zhou, Y. J., & Yin, W. J. (2022). Molecular mechanisms
for ABCA1-mediated cholesterol efflux. Cell cycle (Georgetown, Tex.), 21(11), 1121–1139.
Plösch, T., Kruit, J. K., Bloks, V. W., Huijkman, N. C., Havinga, R., Duchateau, G. S., Lin,
Y., & Kuipers, F. (2006). Reduction of cholesterol absorption by dietary plant sterols and stanols
in mice is independent of the Abcg5/8 transporter. The Journal of nutrition, 136(8), 2135–2140.
Kris-Etherton, P. M., Petersen, K., & Van Horn, L. (2018). Convincing evidence supports
reducing saturated fat to decrease cardiovascular disease risk. BMJ nutrition, prevention &
health, 1(1), 23–26.
Arthur, N. C., MaryGisel, N. U., Obia, O., & Ogadinma, N. I. (2022). GC-MS Analyzed
Phytochemicals and Justicia Carnea Remediation of Cadmium-Induced Oxidative Stress,
Hyperglycaemia, and Hyperlipidaemia in Male Wistar Rats. Himalayan Journal of Applied
Medical Sciences and Research. 3(1): 88-95.
Ray, K. K., Troquay, R. P. T., Visseren, F. L. J., Leiter, L. A., Scott Wright, R.,
Vikarunnessa, S., Talloczy, Z., Zang, X., Maheux, P., Lesogor, A., & Landmesser, U. (2023).
Long-term efficacy and safety of inclisiran in patients with high cardiovascular risk and elevated
LDL cholesterol (ORION-3): results from the 4-year open-label extension of the ORION-1 trial.
The lancet. Diabetes & endocrinology, 11(2), 109–119.

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