IIARD International Institute of Academic Research and Development

INTERNATIONAL JOURNAL OF MEDICAL EVALUATION AND PHYSICAL REPORT (IJMEPR )

E-ISSN 2579-0498
P-ISSN 2695-2181
VOL. 8 NO. 5 2024
DOI: 10.56201/ijmepr.v8.no5.2024.pg83.89

---

The Effect of Hydro-Methanolic Seed Extract of Azanza garckeana (Goron Tula) on Lipid Profile and Oxidative Stress Markers of Male Wistar Rats

Onyebuchi Obia, Hannah Ezekiel-Hart, Joy O. A Laz-Okenwa, Edith Reuben, Dagbota Dan-Jumbo and Chisom F. Wami-Amadi

---

Abstract

The prevalence of cardiometabolic conditions has risen globally over the years even in the low- and middle-income countries. The present study was aimed at investigating the effects of hydro- methanolic seed extract of Azanza garckeana on the lipid profile and oxidative stress markers of male wistar rats. A total of 20 wistar rats were separated into four groups of five rats each; including group 1 which served as control. Groups 2, 3, and 4 respectively received a daily dose of 250mg/kg, 500mg/kg, and 1000mg/kg of the extract. The body weight of the animals was taken on day 1 and day 28. The experiment lasted for 28 days, thereafter, the animals were sacrificed and blood samples collected for the determination of lipid profile and oxidative stress markers. The result showed that 1000mg/kg of the extract caused a significant increase in total cholesterol, low-density lipoprotein, and very low-density lipoprotein levels but no significant effect on the triglyceride, and high-density lipoprotein. The percentage of weight gain was significantly reduced compared to control. 250mg/kg and 500mg/kg of the extract did not cause significant changes in the lipid profile parameters. There were also no significant changes in the oxidative stress parameters. This therefore suggests that at higher doses hydro-methanolic seed extract of Azanza garckeana despite causing dyslipidaemia, may have a weight protective effect and would not potentially increase lipid peroxidation in male wistar rats.

keywords:

Azanza garckeana, lipid profile, oxidative stress markers.

---

References:

1. Yusuf, A. A., Lawal, B., Sani, S., Garba, R., Mohammed, B. A., Oshevire, D. B., & Adesina,
D. A. (2020). Pharmacological activities of Azanza garckeana (Goron Tula) grown in
Nigeria. Clinical Phytoscience, 6(1). https://doi.org/10.1186/s40816-020-00173-0
Michael, K. G., Onyia, L. U., & Jidauna, S. B. (2015). Evaluation of Phytochemicals in
Azanza garckeana (Gorontula) Seed. Journal of Agriculture and Veterinary Science
Volume, 8(5), 71–74.
Nkafamiya, I. I., Ardo, B. P., Osemeahon S. A. and Akinterinwa, A. (2016). Evaluation of
Nutritional, Non-nutritional, Elemental Content and Amino Acid Profile of Azanza
garckeana (Goron Tula). British Journal of Applied Science & Technology, 12(6): 1-10.
Ochokwu, I. J., Dasuki, A., & Oshoke, J. O. (2015). Azanza garckeana (Goron Tula) as an
edible indigenous fruit in North Eastern Part of Nigeria. Journal of Biology, Agericulture
and Healthcare, 5(15), 26-31.
Maroyi, A. (2017). Azanza garckeana Fruit Tree: Phytochemistry, Pharmacology, Nutritional
and Primary Healthcare Applications as Herbal Medicine: A Review. Research Journal of
Medicinal Plants, 11(4), 115–123..
Ajiboso, S.O. (2023). Determination of chemical profile of Azanza garckeana Fruit.
International Journal of Recent Research in Thesis and Dissertation. 4(2): 1–6.
Yusuf, A. A., Lawal, B., Alozieuwa, U. B., Onikanni, A. S., Lukman, H. Y., Fadaka, A. O.,
Olawale, F., Osuji, O., Sani, S., Owolabi, M. S., Adewuyi, A. H., Yusuf, D. H., Batiha, G.
E., Ataya, F. S., & Fouad, D. (2023). Attenuating effects of Azanza garckeana fractions on
glycemo-impaired-associated dyslipidemia, hepatopathy, and nephropathy. American
journal of translational research, 15(10), 5997–6014.
Pizzino, G., Irrera, N., Cucinotta, M., Pallio, G., Mannino, F., Arcoraci, V., Squadrito, F.,
Altavilla, D., & Bitto, A. (2017). Oxidative Stress: Harms and Benefits for Human
Health. Oxidative medicine and cellular longevity, 2017, 8416763.
Tepeba??, M. Y., ?lhan, ?., Temel, E. N., Sancer, O., & Öztürk, Ö. (2023). Investigation of
inflammation, oxidative stress, and DNA damage in COVID-19 patients. Cell stress &
chaperones, 28(2), 191–199.
Qureshi, N. Q., Mufarrih, S. H., Bloomfield, G. S., Tariq, W., Almas, A., Mokdad, A. H.,
Bartlett, J., Nisar, I., Siddiqi, S., Bhutta, Z., Mark, D., Douglas, P. S., & Samad, Z. (2021).
Disparities in Cardiovascular Research Output and Disease Outcomes among High-,
Middle- and Low-Income Countries - An Analysis of Global Cardiovascular Publications
over the Last Decade (2008-2017). Global heart, 16(1), 4.
Sarmadi, B., Musazadeh, V., Dehgham, & V., Karimi, E., (2023). The effect of cinnamon
consumption on lipid profile, oxidative stress, and inflammation biomarkers in adults: An
umbrella meta-analysis of randomized controlled trials, Nutrition, Metabolism and
Cardiovascular Diseases. 33 (10): 1821-1835.
Dadaya, E., Koubala, B., Ndjonka, D., Zingué, S., Laya, A., & Atsang, G. (2023).
Hydromethanolic Root Extract of Gnidia Kraussiana Demonstrates Anti-Inflammatory
Effect Through Anti-Oxidant Activity Enhancement in a Rodent Model of Gout. Dose-
response : a publication of International Hormesis Society, 21(1), 15593258221148015.
Obia, O., Ogwa, C. O., Ojeka, S. O., Ajah, A. A., & Chuemere, A. N. (2016). Effect of honey
on the body weight of glibenclamide trested diabetic wistar rats. Journal of Apitherapy. 1
(1): 33-35.
Lawal, B., Sani, S., Onikanni, A. S., Ibrahim, Y. O., Agboola, A. R., Lukman, H. Y.,
Olawale, F., Jigam, A. A., Batiha, G. E., Babalola, S. B., Mostafa-Hedeab, G., Lima, C. M.
G., Wu, A. T. H., Huang, H. S., & Conte-Junior, C. A. (2022). Preclinical anti-
inflammatory and antioxidant effects of Azanza garckeana in STZ-induced glycemic-
impaired rats, and pharmacoinformatics of it major phytoconstituents. Biomedicine &
pharmacotherapy = Biomedecine & pharmacotherapie, 152, 113196.
Gao, S., Zhao, D., Qi, Y., Wang, W., Wang, M., Sun, J., Liu, J., Li, Y., & Liu, J. (2018).
Circulating Oxidized Low-Density Lipoprotein Levels Independently Predict 10-Year
Progression of Subclinical Carotid Atherosclerosis: A Community-Based Cohort Study.
Journal of atherosclerosis and thrombosis, 25(10), 1032–1043.
Iyojo, I. J., Ibrahim, R. P., Tagang, A., Allam, L., & Olusegun, A. J. (2022). Effects of
different extracts of Azanza garckeana fruit pulp on haematological and biochemical
parameters of New Zealand White (NZW) rabbit bucks. Comparative Clinical Pathology,
31, 453–463.
Itodo, J. I., Ayo, J. O., Rekwot, I. P., Aluwong, T., Allam, L., & Ibrahim, S. (2022).
Comparative Evaluation of Solvent Extracts of Azanza Garckeana Fruit Pulp on Hormonal
Profiles, Spermiogram and Antioxidant Activities in Rabbit Bucks. World Rabbit Science,
30(4), 309–326. https://doi.org/10.4995/WRS.2022.17256
Bakr, A. F., & Farag, M. A. (2023). Soluble Dietary Fibers as Antihyperlipidemic Agents: A
Comprehensive Review to Maximize Their Health Benefits. ACS omega, 8(28), 24680–
24694.

DOWNLOAD PDF

---

Back