IIARD International Institute of Academic Research and Development

International Journal of Agriculture and Earth Science (IJAES )

E- ISSN 2489-0081
P- ISSN 2695-1894
VOL. 10 NO. 6 2024
DOI: 10.56201/ijaes.v10.no6.2024.pg9.21

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In Vitro Control of Dry-Rot Disease Associated with Solanum tuberosum L. Using Varied Concentrations of Crude Extracts of Daldinia cocentrica (James BOLTON)

Nmom, F. W. , Wofu, N. B. and Agbagwa S. S.

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Abstract

Solanum tuberosum L. ((Irish Potato), popularly described as vegetable king, is a staple world’s most productive crop. It is the source of nutrients such as crude protein, dietary fibres, anti-oxidants; such as vitamin C. Its availability and sustainability is threatened by post harvest deterioration such as dry-rot disease. This study was intended to in vitro control the disease, using varied concentrations (25%, 50% and 100%) of crude extract of Daldina concentrica an endophytic fungus; known for its mycochemical actions against phytopathogenic fungal attacks on crops. The agar well food poison method of Martinus Berjerinck was used for the study. The results obtained from the study revealed that with 25% concentrations of the control agent; there was no impact of inhibition of the spread of the growth of the pathogen; however, 50% concentration showed an inhibition of 8mm and 100% concentration gave an inhibition of 12mm against the spread of the pathogen. This is an indication that the use of the control agent at higher concentrations can inhibit the growth of Fusarium oxysporium; the causal agent of potato dry-rot disease. It is likely that the control agent was able to inhibit the spread of the pathogen and inhibited the disease development; possibly due to the mycochemicals contained in the extract of the control agent.

keywords:

P. solanum tuberosum, Daldina concentrica, Crude extracts, In Vitro, Agar

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References:

Abbas, G. F., Hafiz, I. A., Hussain, N. A. and Shabbir, G. (2011). Assessment of processing
and nutritional quality of potato genotypes in Pakistan. Pakistan Journal of
Agriculture Science, 48: 169 – 175.
Adrian, T. E., Pasquali, C., Pescosta, F., Bacarese – Hanulton, A. J. and Bloom (1976).
Nutritional and Toxicological significance of enzyme inhibitors in foods. Springer
link.
Adrian, M., Jeandet, P., Veneay, J. I., Western, L. A., and Besis, R. (1977). Biological
activity of re: a stilbene compound from grapes vines against Botrytis, cinerea, the
causal agent of grey mold. Journal of Chemical Ecology, 23: 1689 – 1703.
Aiko, V. and Mehta, A. (2016). Prevalence of toxigenic fungi in common medicinal herbs
and spices in India. Biotechnology, 6, 159. https://doi:10-1007/5/3205-016-476-9.
Al-Mughrabi, K. I., Vikram, A. and Peters, R. D. (2013). Efficacy of Pseudomonas syringae
in the management of potato tuber diseases in storage. Biological control, 64: 315-
https://doi.org/10.1016/biocontrol.
Armah, C. N., Mackie, A. R., Roy, C., Price, K., Osbourn, A. E., Bowyer, P. and Lahda, S.
(1999). The membrane permeabilizing effect of avenacin – 1 involves the
reorganization of bilayer cholesterol. Biophysics Journal, 76: 281 – 290.
Adym, M. H. (2019). Evaluatino of some Trichoderma species in biological control of
potato dry-rot caused by Fusarium sambucinum fucked isolates. Applied Ecological
Environment Research, 17(1): 533-546.
Bajanowski, J. S., Donatolli, M. and Skidmore, A. K. (2013). An autocalibration procedure
for empirical solar radiation models: modelling and software – Elsevier.
Chang, K. F., Hwuang, S. F., Conner, R. L., Ahmed, H. U., Zhou, Q., Turnbull, G. D.,
Strekov, S. E., Mclaren, D. L. and Gossen, B. D. (2013). Comparison of PCRs and
RAPD markers for identification of Fusarium solani f.sp Phaseoli isolates from dry
beans root rot. In: Plant Pathology Journal.
Cheesebrough, M. (2006). District laboratory practice in tropical countries. Cambridge
University Press. 2 (2): 1 – 434.
Croteau, R., Kut Chian, T. and Lewis, W. (2000). Natural products (secondary metabolites).
In: Buchaman, B., Gruiseem, W. Joneas, R. (eds). Biochemistry and molecular
Biology of Plant Biologists. Rockville, M. D. pp: 1250 – 1268.
Crotaras, M. Folch, C., Mendoza, L. (2004). Characteristics of antifungal activity on
Botrytis cenerea of the natural deterpnoids kraurenoic acid 3B-hydrozy-krauronoic
acid. Journal of Agriclture. Food Chemistry, 52: 2821-2826.
Daami-Remadi, M., Jabneun-khiareddine, H., Aged, F. and El-mahjoul, M. (2012). Effect
of temperature on aggressivity of Tunifian Fusarium species causing potato
(Solanum tuberosum) tuber dry rot. Agronomy Journal, 5: 350 – 355.
De-langa, J. H. Vincent, H. P., Du Plessis, L. M., Vanwyk, P. J. and Ackerman, L. G. J.
(1976). Scoparone (6, 7 – dimethoxycoumanin) induce in citrus peel by black spot,
Guigmardia citripaca Kidy. Phytophylactica, 8: 83 – 84.
Ejikeme, M., Ezeonu, C. S. and Eboatu, A. N. (2014). Determination of physical and
phytochemical constituents of some tropical timbers indigenous to Niger Delta area
of Nigeria. European Scientific Journal, 10(18): 1857 – 788.
Elshering E. A., Anium, B. H. and Baka, Z. H. (2016). Efficiency of pomegranate (Punica
granatum L) Peels extract as a high potential natural tool towards Fusarium dry-rot
on potato tubers. Postharvest Biology and Technology, Elsevier.
Ezeonu, C. S. (2016). Qualitative and Quantitative determination of Phytochemical
Contents of Indigenous Nigerian Soft Woods. New Journal of Science, Vol. 2016
http://doi.org/10.1155/2016/5601327.pp: 9.
FAOSTAT (2008). Food and Agriculture organization data statistics (2018).
https://wwfao.org/faostat/en/#data/ Qcinfo,
Fischer, N. L., Burgess, L. W., Toussoun, T. A. and Nelson, P. E. (1982). Carnation leaves
as a substrate and for preserving cultures of Fusarium species. Phytopathology, 72:
151 – 153.
Gillitzer, P. A., Wyse, D., Sheaffer, C. C. and Lehmaro, C. C. (2012). Optimi9zation of
screening of native and naturalized plants from Minnesota for antimicrobial activity.
Researchgate, Doi.10.5897/JMPR10:710.
Harbourne, I. B. (1973). Phytochemical methods. A guide to modern techniques of plant
analysis. 2nd edition. Chapman and Hall, New York: 88 – 185.
Heltoft, P., Wold, A. B. and Molteberg, E. L. (2015). Maturity indicators for prediction of
potato (S. tuberosum L) quality during storage. Postharvest Biology and Technology.
Vol. 129: 97 – 106.
Irish seed sowers (2010).
Kolaei, E. A., Cenatus, C., Tweddell, R. J. and Avis, T. J. (2013). Antifungal activity of
aluminium-containing salts against the development of carrot cavity spot and potato
dry-rot. Annals of Applied Biology, 163: 311-317.
Latus-Zietkiewicz, D., Perkowski, J., Chelkowski, J. (1987). Fusarium species as pathogens
of potato tubers during storage and their ability to produce mycotoxins. Mycotoxin
Research, 3(1): 99 – 104.
Mahmoud, N., Salabi, F., Zhang Li; Zhao, F., Wei, C. and Lixin D. U. (2010). AAV2 –
mediated follestatin overpression induces ovine primary myoblasts proliferation.
BMC Biotechnology, 14: 87 http:/www.biomecentral.com/1472-6750/14/87.
Maria-Fernanda, T., Sidele, F., Mondello, L. and Rigano, F. (2019). The contributions of
carotenoids phenolic compounds and flavonoids to the antioxidative properties of
marine microalgae isolated from Mediterranean morocco. Molecules, 24(22): 4037.
Mopi.com.
Martinez, A. J. (2012). Natural fungicides obtained from plants. In: fungicides for plants
and animals. Available at http://www.interopen.com/boost/fungicides for plants and
animals/natural fungicides from plants.
Munro, A. B. (2000). Oxalate in Nigerian vegetables West African Journal of Biological
Application Chemistry, 12 (1): 14 – 18.
NCLS: National committee on laboratory standards (2002).
Obadoni, B. O. and Ochuko, P. O. (2001). Phytochemical studies and comparative efficacy
of the crude extracts of some Homeostatic plants in Edo and Deta States of Nigeria.
Global. Journal of Pure and Applied Science, 8: 203 – 208.
Railes, R. (1992). Effect of chromium chloride supplementation on glucose tolerance and
serum lipids including HDL of adult men. American Journal of Clinical Nutrition,
34: 697 – 700.
Rocha, V., Labopin, M., Sanz, G., Arcese, W. and Schwerdt-feger, R. (2004). Transplants
of umblical-cord blood or bone marrow from unrelated donors in adults with acute
leukemia. New England Journal of Medicine, 351 (22): 2276 – 2285.
Romanazzi, A., Amnaon, L., Gabler, F. M. and Joseph, S. (2012). Fusarium solani isolates
from dry rot of potato and their virulence on different potato cultivars. In: Plant
Pathology. January Postharvest Biology and Technology, 63(1): 141 – 147.
Russell, J. A. (1980). A description of the affective quality attributed to environments.
Journal of Personality and Spical Psychology Psynet. Apa.org, 38(2): 311.
Sofowore, A. (1973). Phytochemical screening of medicinal plants and traditional medicine
in Africa. Edition. Spectrum books ltd. Nigeria pp: 150 – 156.
Stevenson, W. R., Loria, R., Franc, G. D. and Weingartner D. P. (2001). Antifungal activity
of sulfur – containing salts against the development of carrot cavity spot and potato
dry rot. Postharvest Biological Technology, 63(1): 55 – 39.
Tiwari, J. K., Buckseth, T. Zinta, R., Savaswati, A., Singh, P. K. and Rawat, S. (2020).
Genome wide identification and characterization of microRNAs by small RNA
sequencing for low nitrogen stress in potato PLOS ONE 15:e0233026
doi:10.1371/journalpone. 0233076.

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