IIARD International Institute of Academic Research and Development

RESEARCH JOURNAL OF PURE SCIENCE AND TECHNOLOGY (RJPST )

E-ISSN 2579-0536
P-ISSN 2695-2696
VOL. 7 NO. 1 2024
DOI: https://doi.org/10.56201/rjpst.v7.no1.2024.pg82.109

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Determination of Optimal Temperature and PH of Lipase Enzyme Extracted from Beni Seed and Bambara Nuts

Egbi, T.K., Emelogu, J.N., Ahiaukwu, J.A., Inah, N.U., Nwosu-Ezeonye, P.O., Okon, I.J.

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Abstract

Lipase is an enzyme that catalyzes the hydrolysis of ester bonds in substrates such as phospholipids, triglycerides and cholesteryl esters. Lipase (E.C. 3.1.1.3) was extracted from germinating bambara groundnut and beniseed and assayed using 0.1M phosphate buffer pH 8. The crude lipases showed optimum activity at pH 8.0 and temperature of 50

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