IIARD International Institute of Academic Research and Development

RESEARCH JOURNAL OF PURE SCIENCE AND TECHNOLOGY (RJPST )

E-ISSN 2579-0536
P-ISSN 2695-2696
VOL. 7 NO. 1 2024
DOI: https://doi.org/10.56201/rjpst.v7.no1.2024.pg66.81

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Isolated from Soil for Enhanced Production of Alpha Amylase

Egbi, T.K., Ijah U.J.J., Emelogu, J.N., Ahiaukwu, J.A., Asake, O.J., Ayegba, S.O.

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Abstract

Bacillus species capable of extracellular amylase production were isolated from soil samples using rice bran and wheat bran as substrates. Using starch hydrolysis plate assay test the bacterial isolates were screened and were identified using maximum zone of clearance and further characterized according to standard methods and molecular analysis.The isolates were identified as Bacillus safensis. They were used to produce ?-amylase. Bacillus safensis had maximum enzyme activity of 40.86 IU/mL and 43.87 IU/mL on rice and wheat bran respectively over the same period. Enhancement of enzyme production was done using two techniques; induction of mutation by UV irradiation and recombinant DNA technology. Six mutants each were randomly selected from each UV treatment time range. The mutant BSM

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