IIARD International Institute of Academic Research and Development

INTERNATIONAL JOURNAL OF CHEMISTRY AND CHEMICAL PROCESSES (IJCCP )

E-I SSN 2545-5265
P- ISSN 2695-1916
VOL. 10 NO. 1 2024
10.56201/ijccp.v10.no1.2024.pg79.100

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Triterpenes and Steroidal Compounds from Napoleona imperialis Seed: Spectroscopic Approach and Biological Activities Against Some Clinical Isolates

Nna, P.J, Irimiagha, C.G. and Legborsi, J.

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Abstract

This study seeks to isolate, characterize and identify the bioactive compounds from Napoleona imperialis seed and its biological activities against some clinical pathogens. The extraction method employed was cold maceration whereby dried pulverized material (1 kg) was introduced into a 2.5 L Winchester bottle and hexane (1000mL) for 48 hours and the extract was intermittently shaken and was filtered into a clean one litter glass jar, and evaporated to dryness. The same procedure was done for ethyl acetate and methanol successively. Using standardized techniques, wagner reagent, mayer’s reagent, dragendoff reagent and chemical tests such as froth, lead subacetate, ferric chloride, sodium hydroxide and salkowski tests were performed on Napoleona imperialis seed extract in this work to identify secondary metabolites such as alkaloid, flavonoid, steroid, phenol, tannins and saponins which accounts for its medicinal value. In Column chromatography techniques, the plant extracts of n-hexane, ethyl acetate and methanol were dried in sterile stainless steel plates and weighed using a weighing balance. The plant extract sample (2.5 g) of each extract was absorbed unto silica gel (2.0 g) and dried till a free flowing powder was obtained. This was done in order to get a pure and refined isolates. The thin layer chromatography (TLC) technique was used to the concentrated fractions that were obtained from the column chromatography. After careful application, a spot of each fraction was placed on a TLC plate that had been coated with silica. To determine the molecular structure of the compounds, a Pasteur pipette was used to dissolve the cleaned samples in chloroform (CDCl3) before they were transferred to clean NMR tubes and analyzed using nuclear magnetic resonance (NMR). To ascertain the anti-microbial activity, selected organisms (Bacteria isolates) were examined in the study. The organism was maintained on nutrient Broth for 24 hours. For the purpose of standardising the tes

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